Myconanotechnology to Treat Infectious Diseases: A Perspective

Castro-Longoria, Ernestina; Garibo-Ruíz, Diana; Martínez-Castro, Sandy

doi:10.1007/978-3-319-68424-6_12

Cited by 7 publications

(9 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in the chemical synthesis of CuONPs, there is a generation of residues considered harmful to health and the environment [ 18 ]. Considering that the use of metallic nanoparticles has promising applications in biomedicine, it is important to search for efficient NPs to use low concentrations and safe, reducing agents for the synthesis protocol [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is necessary to look for new treatments to combat the resistance of these microorganisms. Nanotechnology offers novel alternatives to combat pathogenic microorganisms since metallic nanoparticles such as silver, copper, and gold, among others, have antimicrobial properties [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the antimicrobial activity of copper-based nanoparticles has not been fully studied, but the antimicrobial effectiveness of chemically synthesized copper nanoparticles has also been reported, including against C. albicans [ 9 ]. However, chemical synthesis could contain toxic residues, and then, green synthesis can be a better option for biomedical applications such as their use as antimicrobials [ 5 ]. In fact, a clear advantage has been demonstrated in using biosynthesized copper nanoparticles against C. albicans [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Highly Antifungal Activity of Biosynthesized Copper Oxide Nanoparticles against Candida albicans

García-Marín

Juárez-Moreno²,

Castro-Longoria

2022

Nanomaterials

Self Cite

View full text Add to dashboard Cite

Candida albicans (ATCC SC5314) was exposed to biosynthesized copper oxide nanoparticles (CuONPs) to determine their inhibitory capacity. Nanoparticles were polydisperse of small size (5.8 ± 3.5 nm) with irregular shape. The minimum inhibitory concentration (MIC) against C. albicans was 35.5 µg/mL. The production of reactive oxygen species (ROS) of C. albicans was verified when exposed to different concentrations of CuONPs. Ultrastructural analysis of C. albicans revealed a high concentration of CuONPs in the cytoplasm and outside the cell; also, nanoparticles were detected within the cell wall. Cytotoxic analyses using fibroblasts (L929), macrophages (RAW 264.7), and breast (MCF-12) cell lines show good results of cell viability when exposed at the MIC. Additionally, a hemocompatibility analysis was carried out and was found to be below 5%, considered the threshold for biocompatibility. Therefore, it is concluded that the biosynthesized CuONPs have a high potential for developing a topical antifungal treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly Antifungal Activity of Biosynthesized Copper Oxide Nanoparticles against Candida albicans

García-Marín

Juárez-Moreno²,

Castro-Longoria

2022

Nanomaterials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biogenic AgNPs are potentially useful in the clinic; they have been proposed as antimicrobial agents to offer an alternative treatment to solve problems of resistance to antibiotics traditionally used against pathogenic strains [ 10 , 11 ]. However, for the application of biogenic NPs in the medical area, it is necessary that the biological material used for the synthesis does not come from pathogenic microorganisms, containing potentially toxic molecules for humans [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Optimized Synthesis of Small and Stable Silver Nanoparticles Using Intracellular and Extracellular Components of Fungi: An Alternative for Bacterial Inhibition

Murillo-Rábago

Juárez-Moreno²,

Garcia-Marin

et al. 2022

Antibiotics

Self Cite

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) represent an excellent option to solve microbial resistance problems to traditionally used antibiotics. In this work, we report optimized protocols for the production of AgNPs using extracts and supernatants of Trichoderma harzianum and Ganoderma sessile. AgNPs were characterized using UV-Vis spectroscopy and transmission electron microscopy, and the hydrodynamic diameter and Z potential were also determined. The obtained AgNPs were slightly larger using the fungal extract, and in all cases, a quasi-spherical shape was obtained. The mean sizes of AgNPs were 9.6 and 19.1 nm for T. harzianum and 5.4 and 8.9 nm for G. sessile using supernatant and extract, respectively. The AgNPs were evaluated to determine their in vitro antibacterial effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The minimum inhibitory concentration (MIC) was determined, and in all cases the AgNPs showed an antimicrobial effect, with a MIC varying from 1.26–5.0 µg/mL, depending on the bacterial strain and type of nanoparticle used. Cytotoxicity analyses of AgNPs were carried out using macrophages and fibroblast cell lines. It was determined that the cell viability of fibroblasts exposed for 24 h to different concentrations of AgNPs was more than 50%, even at concentrations of up to 20 µg/mL of silver. However, macrophages were more susceptible to exposure at higher concentrations of AgNPs as their viability decreased at concentrations of 10 µg/mL. The results presented here demonstrate that small AgNPs are obtained using either supernatants or extracts of both fungal strains. A remarkable result is that very low concentrations of AgNPs were necessary for bacterial inhibition. Furthermore, AgNPs were stable for more than a year, preserving their antibacterial properties. Therefore, the reported optimized protocol using fungal supernatants or extracts may be used as a fast method for synthesizing small AgNPs with high potential to use in the clinic.

show abstract

“…In the near future, myconano-functional agrochemical products, crop protection pre-harvest and post-harvest, sensing systems and genetic equipment will be enhanced for direct use in farms and fields [12]. Fungal-mediated nanoparticles have shown effective inhibition against pathogens causing infectious diseases in humans, especially against those deemed multi-resistant to traditional antibacterial agents [13]. Fungi-based nanosorbents are also a novel study path in the field of heavy metal biosorption from wastewater pollutants [14].…”

Section: Introductionmentioning

confidence: 99%