Mycological study on cholesteatoma keratin obtained during primary mastoid surgery

Effat, Kamal G; Madany, N M

doi:10.1017/s0022215114002059

Cited by 9 publications

(11 citation statements)

References 23 publications

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“…Chi squared value: 1.064, P-value 0.302 Discussion In this study, the sample size was 25 patients of either gender with male preponderance, which is smaller than Singh 11 (40 patients) and more than Effat 13 (18 patients), with nearby same age groups in both studies. In this study all patients complained from otorrhoea, in 11 patients associated with hearing loss, 3 patients with vertigo which are similar to Singh study.…”

Section: Table Vi: Summary Of Association Of Fungal Infections With Complicationsmentioning

confidence: 67%

“…There was no bacterial growth in 16% of the specimen in this study which is corresponding to Attallah 12 . Regarding mycology, its incidence in cholesteatomatous CSOM is variable as low as 1.4% in Ricciardiello study 10 to 89% in Effat 13 . In this study, the fungal infestation was detected in 7 of our 25 cases (28%), 8 fungal specious were isolated, Aspergillus is the commonest one (62.5%) followed by candida in 25%.…”

Section: Table Vi: Summary Of Association Of Fungal Infections With Complicationsmentioning

confidence: 96%

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Clinico-Microbiology of Cholesteatoma Obtained During Mastoid Surgery in Patients With Chronic Suppurative Otitis Media

Ali¹,

Al-Shareda²

2021

BJSRG

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Chronic Otitis Media (COM) is a long standing inflammation of the middle ear and mastoid, it is a common health problem. The evidence of microbiology in COM in different studies showed variable frequencies, but little studies showed the role of fungal colonization. This study aimed to identify the microbiology of cholesteatomatous COM concentrating on clarification of fungal colonization. A prospective study was done in otolaryngology department of Basrah Teaching Hospital from June 2018 till July 2019. This study was carried on a randomly collected data of 25 patients diagnosed as COM by a specific designed questionnaire including history and examination. The specimen was collected during mastoid surgery which included any cholesteatoma in the middle ear and mastoid and sent for bacteriological and mycological examination. Statistical study was done by using SPSS v.23 Bacteria were isolated in 84% of the cases in which Pseudomonas aeruginosa was the commonest one (33.3%). Fungi were isolated in 28% cases, in which the Aspergillosis was the commonest (19.3%). There is no recognizable pattern of significant association between the fungi and the reported complications or the stage of the disease. In conclusion, there is significant percentage of fungal colonization in COM with cholesteatoma so it may has a role in inflammatory response of perimatrix, but there is no clear mechanism of this interaction, so may need larger sample and longer duration of study with postoperative follow-up and trail of combined antibiotic-antimycotic treatment.

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Section: Table Vi: Summary Of Association Of Fungal Infections With Complicationsmentioning

confidence: 67%

Section: Table Vi: Summary Of Association Of Fungal Infections With Complicationsmentioning

confidence: 96%

Clinico-Microbiology of Cholesteatoma Obtained During Mastoid Surgery in Patients With Chronic Suppurative Otitis Media

Ali¹,

Al-Shareda²

2021

BJSRG

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“…Previous studies of chronic rhinosinusitis biofilms have revealed the presence of fungal constituents (Healy et al, 2008), alternatively, fungal colonization of cholesteatoma had been sparsely studied. Especially two studies cultured the fungi from cholesteatoma tissue (Effat and Madany, 2014;Singh et al, 2018). Singh et al (2018) cultured 40 cholesteatoma samples and successfully isolated fungi from 17 of the specimens (42.5%).…”

Section: Discussionmentioning

confidence: 99%

“…Among these 17 cases, 6 cases had detectable grown of both fungi and bacteria. Effat and Madany (2014) obtained keratinous debris from 18 patients (19 ears) with cholesteatoma and found culturable fungi in 17 of the samples (89%), but reported no data on culturable bacterial. More recently, Weiss et al (2019) evaluated the microbiota associated with cholesteatoma by 16S rDNA gene sequencing in 19 patients.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and Fungal Infections Promote the Bone Erosion Progression in Acquired Cholesteatoma Revealed by Metagenomic Next-Generation Sequencing

Jiang

et al. 2021

Front. Microbiol.

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An acquired cholesteatoma generally occurs as a consequence of otitis media and eustachian tube dysfunction. Patients with acquired cholesteatoma generally present with chronic otorrhea and progressive conductive hearing loss. There are many microbes reportedly associated with acquired cholesteatoma. However, conventional culture-based techniques show a typically low detection rate for various pathogenetic bacteria and fungi. Metagenomic next-generation sequencing (mNGS), an emerging powerful platform offering higher sensitivity and higher throughput for evaluating many samples at once, remains to be studied in acquired cholesteatoma. In this study, 16 consecutive patients from January 2020 to January 2021 at the Second Affiliated Hospital of Zhejiang University School of Medicine (SAHZU) were reviewed. We detected a total of 31 microbial species in patients, mNGS provided a higher detection rate compared to culture (100% vs. 31.25%, p = 0.000034). As the severity of the patient’s pathological condition worsens, the more complex types of microbes were identified. The most commonly detected microbial genus was Aspergillus (9/16, 56.25%), especially in patients suffering from severe bone erosion. In summary, mNGS improves the sensibility to identify pathogens of cholesteatoma patients, and Aspergillus infections increase bone destruction in acquired cholesteatoma.

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“…9 There are only 3 studies which have cultured fungus from keratin debris, but they report a high prevalence rate of 27% to 89%. [7][8][9] It would also be imperative to note that population explosion and advances in medical care have led to a surge in the incidence of opportunistic fungi, especially in the Asian countries. Hence, a high index of suspicion is necessary for early diagnosis of fungal otomastoiditis, especially in patients with protracted cholesteatoma ear disease presenting with facial nerve palsy.…”

Section: Pathologymentioning

confidence: 99%

A Rare Case of Candida Mastoiditis in an Immunocompetent Patient With Cholesteatoma Ear Disease

2020

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Mycological study on cholesteatoma keratin obtained during primary mastoid surgery

Abstract: Fungal colonisation in middle-ear cleft cholesteatoma probably plays a significant role in disease progression. Moreover, saprophytic fungal colonisation in cholesteatoma keratin may be responsible for the fetor commonly associated with the ear discharge.

Cited by 9 publications

References 23 publications

Clinico-Microbiology of Cholesteatoma Obtained During Mastoid Surgery in Patients With Chronic Suppurative Otitis Media

Clinico-Microbiology of Cholesteatoma Obtained During Mastoid Surgery in Patients With Chronic Suppurative Otitis Media

Bacterial and Fungal Infections Promote the Bone Erosion Progression in Acquired Cholesteatoma Revealed by Metagenomic Next-Generation Sequencing

A Rare Case of Candida Mastoiditis in an Immunocompetent Patient With Cholesteatoma Ear Disease

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