Mycolicibacterium sp. strain PAM1, an alfalfa rhizosphere dweller, catabolizes PAHs and promotes partner-plant growth

Голубев, С. С.; Muratova, A. Yu.; Panchenko, L. V.; Shchyogolev, S. Yu.; Turkovskaya, O. V.

doi:10.1016/j.micres.2021.126885

Cited by 20 publications

(10 citation statements)

References 81 publications

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“…In this study, the promotion of miscanthus growth by members of the Mycolicibacterium and Chitinophaga genera was described for the first time. The ability of mycobacteria to promote the growth of plants was reported previously for Dendrobium moschatum [ 57 ]; Brassica napus [ 58 ]; Triticum sp., Zea mays , Pisum sativum , and Gossypium hirsutum [ 59 ]; Sorghum bicolor [ 60 ]; a mixture of Festuca arundinacea , F. elata , and F. gigantean [ 61 ]; Trifolium repens [ 62 ]; and Medicago sativa [ 39 ]. The presence of signs of PGPR, including the synthesis of the phytohormone IAA, the dissolution of phosphates, the synthesis of ACC deaminase, in members of Chitinophaga spp.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Heavy-Metal-Resistant PGPR Inoculants on Growth, Rhizosphere Microbiome and Remediation Potential of Miscanthus × giganteus in Zinc-Contaminated Soil

et al. 2023

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Microbial-assisted phytoremediation is considered a more effective approach to soil rehabilitation than the sole use of plants. Mycolicibacterium sp. Pb113 and Chitinophaga sp. Zn19, heavy-metal-resistant PGPR strains originally isolated from the rhizosphere of Miscanthus × giganteus, were used as inoculants of the host plant grown in control and zinc-contaminated (1650 mg/kg) soil in a 4-month pot experiment. The diversity and taxonomic structure of the rhizosphere microbiomes, assessed with metagenomic analysis of rhizosphere samples for the 16S rRNA gene, were studied. Principal coordinate analysis showed differences in the formation of the microbiomes, which was affected by zinc rather than by the inoculants. Bacterial taxa affected by zinc and the inoculants, and the taxa potentially involved in the promotion of plant growth as well as in assisted phytoremediation, were identified. Both inoculants promoted miscanthus growth, but only Chitinophaga sp. Zn19 contributed to significant Zn accumulation in the aboveground part of the plant. In this study, the positive effect of miscanthus inoculation with Mycolicibacterium spp. and Chitinophaga spp. was demonstrated for the first time. On the basis of our data, the bacterial strains studied may be recommended to improve the efficiency of M. × giganteus phytoremediation of zinc-contaminated soil.

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Section: Discussionmentioning

confidence: 99%

“…The primary taxonomic identification of strains Pb113 and Zn19 was carried out on the basis of 16S rRNA gene sequence analysis using the EzBioCloud server [ 38 ]. For details, see [ 39 ]. The Pb113 and Zn19 16S rRNA gene sequences are deposited in the GenBank under accession numbers OQ680140 and OQ680520, respectively.…”

Section: Methodsmentioning

confidence: 99%

Effect of Heavy-Metal-Resistant PGPR Inoculants on Growth, Rhizosphere Microbiome and Remediation Potential of Miscanthus × giganteus in Zinc-Contaminated Soil

et al. 2023

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“…In association with plant rhizosphere, Mycolicibacterium sp. strains are reported as potent degraders of various polycyclic aromatic hydrocarbons (PAHs) [ 50 – 52 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

et al. 2023

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Background Di(2-ethylhexyl) phthalate (DEHP) is a widely detected plasticizer and a priority pollutant of utmost concern for its adverse impact on humans, wildlife and the environment. To eliminate such toxic burden, biological processes are the most promising ways to combat rampant environmental insults under eco-friendly conditions. The present study investigated the biochemical and molecular assessment of the catabolic potential of Mycolicibacterium sp. strain MBM in the assimilation of estrogenic DEHP. Results A detailed biochemical study revealed an initial hydrolytic pathway of degradation for DEHP followed by the assimilation of hydrolyzed phthalic acid and 2-ethylhexanol to TCA cycle intermediates. Besides the inducible nature of DEHP-catabolic enzymes, strain MBM can efficiently utilize various low- and high-molecular-weight phthalate diesters and can grow under moderately halotolerant conditions. Whole genome sequence analysis exhibited a genome size of 6.2 Mb with a GC content of 66.51% containing 6,878 coding sequences, including multiple genes, annotated as relevant to the catabolism of phthalic acid esters (PAEs). Substantiating the annotated genes through transcriptome assessment followed by RT-qPCR analysis, the possible roles of upregulated genes/gene clusters in the metabolism of DEHP were revealed, reinforcing the biochemical pathway of degradation at the molecular level. Conclusions A detailed co-relation of biochemical, genomic, transcriptomic and RT-qPCR analyses highlights the PAE-degrading catabolic machineries in strain MBM. Further, due to functional attributes in the salinity range of both freshwater and seawater, strain MBM may find use as a suitable candidate in the bioremediation of PAEs.

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“…Burkholderia , belong to the phylum β‐proteobacteria, are a diverse group of gram‐negative bacteria that are widely distributed, whose members occupy various ecological niches and are significant in promoting plant growth in the rhizosphere (Zhong et al, 2022). Mycolicibacterium is a crucial group of bacteria that contribute to the degradation of soil contaminants, specifically polycyclic aromatic hydrocarbons, ultimately promoting soil remediation (Golubev et al, 2021). Paraburkholderia , as Burkholderia bacteria, are capable of activating soil insoluble phosphorus, increasing the soil's available phosphorus content, and are capable of endogenous nitrogen fixation, which further promotes plant growth (Caballero Mellado et al, 2007; Liu, Wang, et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

The effect of intercropping leguminous green manure on theanine accumulation in the tea plant: A metagenomic analysis

Duan,

Wang,

Zhang

et al. 2023

Plant Cell & Environment

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Intercropping is a widely recognised technique that contributes to agricultural sustainability. While intercropping leguminous green manure offers advantages for soil health and tea plants growth, the impact on the accumulation of theanine and soil nitrogen cycle are largely unknown. The levels of theanine, epigallocatechin gallate and soluble sugar in tea leaves increased by 52.87% and 40.98%, 22.80% and 6.17%, 22.22% and 29.04% in intercropping with soybean–Chinese milk vetch rotation and soybean alone, respectively. Additionally, intercropping significantly increased soil amino acidnitrogen content, enhanced extracellular enzyme activities, particularly β‐glucosidase and N‐acetyl‐glucosaminidase, as well as soil multifunctionality. Metagenomics analysis revealed that intercropping positively influenced the relative abundances of several potentially beneficial microorganisms, including Burkholderia, Mycolicibacterium and Paraburkholderia. Intercropping resulted in lower expression levels of nitrification genes, reducing soil mineral nitrogen loss and N2O emissions. The expression of nrfA/H significantly increased in intercropping with soybean–Chinese milk vetch rotation. Structural equation model analysis demonstrated that the accumulation of theanine in tea leaves was directly influenced by the number of intercropping leguminous green manure species, soil ammonium nitrogen and amino acid nitrogen. In summary, the intercropping strategy, particularly intercropping with soybean–Chinese milk vetch rotation, could be a novel way for theanine accumulation.

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Mycolicibacterium sp. strain PAM1, an alfalfa rhizosphere dweller, catabolizes PAHs and promotes partner-plant growth

Cited by 20 publications

References 81 publications

Effect of Heavy-Metal-Resistant PGPR Inoculants on Growth, Rhizosphere Microbiome and Remediation Potential of Miscanthus × giganteus in Zinc-Contaminated Soil

Effect of Heavy-Metal-Resistant PGPR Inoculants on Growth, Rhizosphere Microbiome and Remediation Potential of Miscanthus × giganteus in Zinc-Contaminated Soil

Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

The effect of intercropping leguminous green manure on theanine accumulation in the tea plant: A metagenomic analysis

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