Mycobiota associated with the ambrosia beetleMegaplatypus mutatus: threat to poplar plantations

Metagenomic approach of associated fungi with <i>Megaplatypus mutatus</i> (Coleoptera: Platypodinae)

“…However, an increasing number of studies suggest that certain beetle species can have multiple fungal associates (Batra 1966;Gebhardt et al 2004;Ceriani-Nakamurakare et al 2016). Moreover, Kostovcik et al (2015) suggested that the ambrosia interaction is ecologically dynamic and more species-rich than any other insect-fungus interaction.…”

“…Moreover, Kostovcik et al (2015) suggested that the ambrosia interaction is ecologically dynamic and more species-rich than any other insect-fungus interaction. Ceriani-Nakamurakare et al (2016) characterized the fungal community associated with M. mutatus and Populus deltoides based on culture dependent methods. Those results showed a high fungal diversity that included the ambrosia fungi, Raffaelea spp.…”

“…Also other fungal species were frequently detected as having various ecological roles, members of the Fusarium solani and F. oxysporum species complexes, Graphium basitruncatrum, Coprinellus radians and members of Dipodascaceae family were most commonly found. These taxa represented 94.15% of the fungal species isolated from galleries of M. mutatus highlighting the presence of complex, multitrophic interactions (Ceriani-Nakamurakare et al 2016).…”

“…To our knowledge, metagenomic techniques have not been previously used in investigations of forest pests from Argentina, including Platypodinae species. The aims of this research were to: 1) characterize the fungal communities associated to M. mutatus; 2) compare the culture-independent approach of the present study to a previous culture-based study (Ceriani-Nakamurakare et al 2016), in terms of data recovery related to the composition of fungal community; and to 3) test the geographic and host-tree specificity of the assemblage of fungal communities. This research involved two host trees and three locations.…”

“…In one of these locations, the fungal community has been previously analyzed by culture-dependent methods (Ceriani-Nakamurakare et al 2016). …”