Mycobiota and mycotoxins in Brazilian peanut kernels from sowing to harvest

Gonçalez, Edlayne; Nogueira, Juliana H.C.; Fonseca, Homero; Felício, Joana D.; Pino, Francisco Augusto Burkert Del; Corrêa, Benedito

doi:10.1016/j.ijfoodmicro.2008.01.012

Cited by 71 publications

(36 citation statements)

References 26 publications

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“…Regardless of this, no attempt was made to detect aflatoxin producing species by using rapid molecular techniques in peanuts. (Bhattacharya and Raha, 2002;Gonçalez et al, 2008;Nakai et al, 2008;Pildain et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Aflatoxin Producing Strains of Aspergillus Flavus from Peanut (Arachis Hypogaea)

Hussain

Afzal

Irfan

et al. 2015

Turkish JAF Sci.Tech.

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Aflatoxins are the potential carcinogens produced as secondary metabolites by Aspergillus flavus. They have the ability to contaminate large number of food which ultimately affect the human population. Malt extract agar was selected for the growth of control stains of fungus. The aim of the study was to develop a reliable and quick method for the detection of aflatoxin producing strains in peanuts by using molecular approaches. Total 80 samples of infected peanuts were collected from four different cities of Punjab and checked for their aflatoxin contamination. For aflatoxin detection, three target genes nor1, ver1 and aflR were selected which was involved in the aflatoxin biosynthesis. In all examined cases, 24 out of 80 (30%) samples successfully amplified all three genes indicating aflatoxigenic activity. Discrimination between aflatoxigenic and nonaflatoxigenic strains were also determined on the basis of amplification of these three target DNA fragments. In this study, it was also demonstrated that only specific strains were able to produce the aflatoxin contamination in peanuts.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Aflatoxin Producing Strains of Aspergillus Flavus from Peanut (Arachis Hypogaea)

Hussain

Afzal

Irfan

et al. 2015

Turkish JAF Sci.Tech.

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“…Alatoxins and CPA were also detected simultaneously in kernel samples (11.4%). The co-occurrence of CPA and AFs has been reported by several investigators [11,12,55,56]. In addition, Smith et al [57] demonstrated possible synergistic and cumulative efects of the two mycotoxins [17].…”

Section: Resultsmentioning

confidence: 77%

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

Lavkor¹,

Var²,

Öztemiz³

et al. 2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Alatoxin (AF) and cyclopiazonic acid (CPA) contaminations are very important problems for peanuts and its products. The aim of the study was to detect alatoxin (types B and G) and cyclopiazonic acid (CPA) occurrence and critical periods of toxin production in peanuts collected from diferent research areas of Osmaniye and Adana, Turkey, in 2015. Peanut kernels toxin analysis was performed in four diferent periods during the harvest, drying, prestorage, and storage. Total alatoxin occurrence in peanut kernels was analyzed by immunoainity chromatography-reversed-phase high-performance liquid chromatography (IAC-HPLC) analysis and cyclopiazonic acid occurrence in peanut kernels was analyzed by thin layer chromatography (TLC). Alatoxin levels in 76 out of 102 contaminated samples were from 0.3 to 1333.42 μg/kg. Cyclopiazonic acid levels in 18 out of 102 peanut samples were from 16.6 to 44.44 μg/kg. An unusual patern of mycotoxin production (alatoxin types B and G simultaneously with CPA) was seen in 11 of 102 peanuts samples. Six of nine samples were from the storage period. Alatoxin contamination during harvesting (64%) and drying (75%) were higher than prestorage (53%). Alatoxin (93%) and cyclopiazonic acid (30%) were the most produced during storage. The results showed that storage period was signiicantly important for the presence of two mycotoxins according to the statistical analysis.

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“…Several analytical techniques have been used either singly or in combination for detection of aflatoxins such as TLC, HPLC, Enzyme linked immune sorbant assay (ELISA) and Fluorometry. For many years there has been much interest focused on detection and quantification of Aspergillus species from section of A. flavus which are responsible for contamination of agricultural products such as groundnuts, peanuts and other crops [26,27,28]. In the present study, aflatoxin producing fungi were isolated from infected ground nut seeds and screened for their ability to produce toxins by CCA and primarily detected by TLC.…”

Section: Molecular Identification Of Isolated Strainmentioning

confidence: 99%

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

2016

IJCBS

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Aflatoxin contamination in food is a major qualititative problem affecting human health and trade. Detoxification of these aflatoxins has been considered as one of the best strategies to decontaminate the food and animal feed. The aim of the present study was to isolate, identify and detoxify the aflatoxin produced by A.flavus from infected ground nut. The present study was focused on isolation, identification of A. flavus from infected groundnut pods on Czapek Dox Agar medium, and the toxigenic strains were confirmed on Coconut Cream Agar Medium (CCA). Aqueous extracts of C. citratus were tested against aflatoxin producing strains of A. flavus at various concentrations (1, 2.5 and 5% v/v). Further, the detoxifying capacity of these leaf extracts was studied at these concentrations on AFB1 using TLC and HPLC. The toxigenic strain of A. flavus was confirmed based on PCR amplification. Results indicated that C. citratus extracts exhibited high antifungal activity showing inhibition of fungal mycelium to a maximum extent at 5% v/v concentration. However, the other two concentrations of C. citratus were also found to be effective in inhibition of A. flavus mycelia. Detoxification studies indicated lesser levels of AFB1 at all the concentrations under study. Maximum degradation of the toxin was obtained at 5% v/v concentration from 2154 μg/L to 183 μg/L. It was observed that the extract of C. citratus showed significant detoxification and inhibitory effect of growth of A. flavus. Our future studies are directed in characterizing the active principle of the C. citratus plant extract and application of these extracts on detoxification of Aflatoxins.

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Mycobiota and mycotoxins in Brazilian peanut kernels from sowing to harvest

Cited by 71 publications

References 26 publications

Molecular Detection of Aflatoxin Producing Strains of Aspergillus Flavus from Peanut (Arachis Hypogaea)

Molecular Detection of Aflatoxin Producing Strains of Aspergillus Flavus from Peanut (Arachis Hypogaea)

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

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