Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species

Abstract

A previously undescribed, slowly growing, scotochromogenic mycobacterium was isolated from a patient with symptomatic pulmonary infection during hsp65 sequence-based identification of Korean clinical isolates. Phenetic characteristics of this strain were generally similar to those of Mycobacterium nebraskense and Mycobacterium scrofulaceum. However, some phenetic characteristics differentiated it from these two species. Its 16S rRNA gene sequences were unique and phylogenetic analysis based on 16S rRNA gene sequences placed the organism in the slowly growing Mycobacterium group close to M. nebraskense and M. scrofulaceum. Its unique mycolic acid profiles and the results of phylogenetic analysis based on two independent alternative chronometer molecules, hsp65 and rpoB, confirmed the taxonomic status of this strain as representing a novel species. These data support the conclusion that this strain represents a novel mycobacterial species, for which the name Mycobacterium seoulense sp. nov. is proposed. The type strain is strain 03-19 T (=DSM 44998 T =KCTC 19146 T ).

Mycobacterium mantenii sp. nov., a pathogenic, slowly growing, scotochromogenic species

“…1), which also are scotochromogenic, slowly growing mycobacteria (Prissick & Masson, 1956;Mun et al, 2007). The hsp65 sequence and, to a lesser extent, the rpoB sequence suggest that our isolates are related to Mycobacterium saskatchewanense, another scotochromogenic, slowly growing NTM (Turenne et al, 2004).…”

Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex

“…As a means of complementing conventional methods, sequence-based classification methods targeting several chronometer molecules such as the 16S rRNA gene (Stahl & Urbance, 1990;Stone et al, 1995), rpoB (Kim et al, 1999(Kim et al, , 2001) and hsp65 genes (Kim et al, 2005(Kim et al, , 2006 have been developed. A polyphasic taxonomic approach containing both conventional and molecular-based methods could provide a conclusive result for the delimitation of novel taxa (Turenne et al, 2004;Mun et al, 2007). The purpose of the present study was to establish the taxonomic position of a 'difficult-to-identify' isolate from a Korean patient with general symptoms of pulmonary infection by using a polyphasic taxonomic approach.…”

“…The purified DNA was used as a template for PCR amplifications of three independent genes as described previously (Mun et al, 2007), i.e. the 16S rRNA gene, heat-shock protein 65 (hsp65) and RNA polymerase (rpoB).…”

Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare

“…Recently, the application of molecular techniques to the taxonomy and identification of isolates from environmental sources and clinical specimens has led to an increased awareness of the diversity within NTM (Magee & Ward, 2012). Because combined molecular and conventional taxonomic methods have been applied to the isolates from Korean patients, we have successfully identified several novel NTM species to date (Kim et al, 2012d(Kim et al, , 2013aLee et al, 2010a, b;Mun et al, 2008Mun et al, , 2007.…”