Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex

Mun, Ho Suk; Park, Joo Hee; Kim, Hong; Yu, Hee Kyung; Park, Young Gil; Chen, Yong; Kook, Yoon Hoh; Kim, Bum Joon

doi:10.1099/ijs.0.65374-0

“…This novel mycobacterium is characterized by a unique phenotypic feature, the pink pigmentation of the colonies, but the major role in its differentiation was played by the genetic analysis that was, at that time, beginning to emerge. The boom years of the MTC start however in 2006 with the description of three novel species Mycobacterium kumamotonense, Mycobacterium senuense and Mycobacterium arupense (Cloud et al, 2006;Masaki et al, 2006;Mun et al, 2008).…”

mentioning

confidence: 99%

Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov.

Tortoli

¹

,

Gitti

²

,

Klenk

³

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species Mycobacterium arupense , three sqvs were detected in the 16S rRNA gene, six sqvs in the hsp65 gene and 15 sqvs in the rpoB gene; in Mycobacterium senuense two sqvs were present in each of the three genetic regions; in Mycobacterium kumamotonense four, two and nine sqvs were found, respectively, and in M. terrae three, four and six sqvs were found, respectively. The inappropriate inclusion of Mycobacterium triviale within the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of M. terrae complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to Mycobacterium hiberniae . The type strain of this species, of which eight strains were investigated, is ATCC 27353T ( = DSM 45694T). A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to M. arupense with respect to the 16S rRNA gene, M. heraklionense sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432T ( = LMG 24735T = CECT 7509T). Mycobacterium longobardum sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the M. terrae complex, clearly divergent from any other species. The type strain is DSM 45394T ( = CCUG 58460T).

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“…Phylogenetic analysis based on 16S rRNA gene sequences supports the genetic disparity of M. triviale from other members of the complex. Recently, polyphasic approaches have broadened the taxonomic diversity of the M. terrae complex, with several novel species added, including Mycobacterium arupense, Mycobacterium hiberniae, Mycobacterium kumamotonense and Mycobacterium senuense (Cloud et al, 2006;Kazda et al, 1993;Lee et al, 2010;Masaki et al, 2006;Mun et al, 2008). However, all recently described members of the complex are related to M. terrae or M. nonchromogenicum rather than to M. triviale.…”

mentioning

confidence: 99%

“…In an effort to differentiate and identify NTM, approximately 150 mycobacterial species have been described to date. Recently, the combination of conventional methods and molecular analyses, especially PCR-mediated sequencing methods, has been successfully applied to describe novel species in the genus Mycobacterium (Lee et al, 2010;Mun et al, 2007Mun et al, , 2008.The Mycobacterium terrae complex comprises several different species that are phenotypically very similar but genetically distinct, classically including M. terrae, Mycobacterium nonchromogenicum and Mycobacterium triviale (Lee et al, 2004;Tortoli, 2003). However, it is now clear that M. triviale is separate from this complex.…”

mentioning

confidence: 99%

“…In an effort to differentiate and identify NTM, approximately 150 mycobacterial species have been described to date. Recently, the combination of conventional methods and molecular analyses, especially PCR-mediated sequencing methods, has been successfully applied to describe novel species in the genus Mycobacterium (Lee et al, 2010;Mun et al, 2007Mun et al, , 2008.…”

mentioning

confidence: 99%

“…Decontamination of the sputa sample and cultivation were as described previously (Lee et al, 2010;Mun et al, 2008).…”

mentioning

confidence: 99%

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Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale

Kim

¹

,

Jeong

²

,

Lee

³

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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Department of Microbiology, School of Medicine, Joong-Ang University, Seoul, Republic of Korea A novel slow-growing, non-chromogenic mycobacterium (strain 01-305 T ) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus Mycobacterium. Phenotypically, strain 01-305 T was generally similar to Mycobacterium triviale ATCC 23292 T , but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305 T was similar to those of M. triviale ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and M. triviale ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of M. triviale ATCC 23292 T (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305 T in the slow-growing Mycobacterium group close to M. triviale ATCC 23290 and M. triviale ATCC 23291, but not M. triviale ATCC 23292 T . Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305 T represents a novel mycobacterial species, for which the name Mycobacterium koreense sp. nov. is proposed. The type strain is 01-305 T (5DSM 45576 T 5KCTC 19819 T ).Mycobacteria are widely distributed in the environment, and some are pathogenic to humans and animals, some being saprophytic. In addition to strict pathogens, including the Mycobacterium tuberculosis complex and Mycobacterium leprae, non-tuberculous mycobacteria (NTM) are able to cause opportunistic infections (Tortoli, 2009). As they generally show species-specific resistance to antibacterial agents, there is an increasing need for precise identification of clinically isolated NTM. In an effort to differentiate and identify NTM, approximately 150 mycobacterial species have been described to date. Recently, the combination of conventional methods and molecular analyses, especially PCR-mediated sequencing methods, has been successfully applied to describe novel species in the genus Mycobacterium (Lee et al., 2010;Mun et al., 2007Mun et al., , 2008.The Mycobacterium terrae complex comprises several different species that are phenotypically very similar but genetically distinct, classically including M. terrae, Mycobacterium nonchromogenicum and Mycobacterium triviale (Lee et al., 2004;Tortoli, 2003). However, it is now clear that M. triviale is separate from this complex. Therefore, taxonomic separation between these species based on conventional biochemical tests is almost impossible, but they can be differentiated into distinct species based on genetic methods. Despite phenotypic similarity between Abbreviations: NTM, non-tuberculous mycobacteria; PNB, p-nitrobenzoate; TCH, thiophene-2-carboxylic acid hydrazide.The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRN...

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Performance evaluation of moving bed bioreactor for simultaneous nitrification denitrification and phosphorus removal from simulated fertilizer industry wastewater

Bhattacharya

¹

,

Mazumder

²

2023

Environ Sci Pollut Res

1

0

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Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex

Cited by 42 publications

References 16 publications

Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov.

Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov.

Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale

Performance evaluation of moving bed bioreactor for simultaneous nitrification denitrification and phosphorus removal from simulated fertilizer industry wastewater

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