Mycobacterium chimaera encephalitis following cardiac surgery in three adult immunocompetent patients: first detailed neuropathological report

Abstract

neuropathology practice and the access of different centres to these tests, we designed a survey that was sent to all members of the Canadian Association of Neuropathology member list in the fall of 2017. This survey asked a number of questions relating to the approach to glioma diagnosis, immunohistochemical/ molecular test ordering patterns, in-house test availability, and need to send out for testing. In this presentation we will present preliminary results from this survey, with a focus on institutional testing capabilities. This provides a valuable resource that could ultimately need to a national database of immunohistochemical and molecular test availability for each neuropathology centre. LEARNING OBJECTIVESThis presentation will enable the learner to:1. Review the key molecular markers in the diagnosis of adult gliomas and methods of testing for them 2. Discuss the effect that the 2016 WHO CNS tumor update has had on clinical practice in Canada ABSTRACT 14Role of MacroH2A2 in the glioblastoma stem cell epigenome Glioblastoma is the most common primary malignant brain tumour in adults, and remains uniformly lethal. These tumours contain a subpopulation of glioblastoma stem cells (GSCs) that drive tumour recurrence and drug resistance. We find that MacroH2A2 is a histone variant that can stratify glioblastoma patients, with higher levels of this histone variant associated with better patient prognosis. Knockdown of macroH2A2 in GSCs is associated with increased self-renewal and an increased expression of stemness genes by RNA-seq. Our preliminary results suggest that macroH2A2 is a novel biomarker for glioblastoma and that macroH2A2 loss is a marker of GSC stemness and a poor prognostic marker in glioblastoma. This work identifies loss of macroH2A2 as a feature of GSCs and provides a framework for therapeutic modulation of this histone variant. LEARNING OBJECTIVESThis presentation will enable the learner to: 1. Explain the role of epigenetics in glioblastoma pathophysiology ABSTRACT 15 Cerebellar glioblastoma: a clinicopathologic series of 16 cases This presentation will enable the learner to: 1. Identify the clinicopathological features of cerebellar GBMs including major molecular alterations 2. Compare cerebellar and supratentorial GBMs and describe the distinguishing features of each type of tumor SESSION 4: Infectious/Immune mediated Neuropathology and Neuromuscular Neuropathology ABSTRACT 16Mycobacterium chimaera encephalitis following cardiac surgery in three adult immunocompetent patients: first detailed neuropathological report.