Mycobacterium as Polycyclic Aromatic Hydrocarbons (PAHs) Degrader

Dudhagara, Dushyant R.; Dave, Bharti P.

doi:10.5772/intechopen.73546

Cited by 12 publications

(7 citation statements)

References 41 publications

(34 reference statements)

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“…Several Mycobacterium strains were human pathogenic species. However, some species, such Mycobacterium austroafricanum and Mycobacterium sp ., have been identified to have the ability to degrade aromatic hydrocarbons [ 62 , 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Potential of Sewage Sludge Mycobiome to Degrade High Diclofenac and Bisphenol-A Concentrations

Conejo-Saucedo

Ledezma-Villanueva

Paz

et al. 2021

Toxics

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One of the most challenging environmental threats of the last two decades is the effects of emerging pollutants (EPs) such as pharmaceutical compounds or industrial additives. Diclofenac and bisphenol A have regularly been found in wastewater treatment plants, and in soils and water bodies because of their extensive usage and their recalcitrant nature. Due to the fact of this adversity, fungal communities play an important role in being able to safely degrade EPs. In this work, we obtained a sewage sludge sample to study both the culturable and non-culturable microorganisms through DNA extraction and massive sequencing using Illumina MiSeq techniques, with the goal of finding degraders adapted to polluted environments. Afterward, degradation experiments on diclofenac and bisphenol A were performed with the best fungal degraders. The analysis of bacterial diversity showed that Dethiosulfovibrionaceae, Comamonadaceae, and Isosphaeraceae were the most abundant families. A predominance of Ascomycota fungi in the culturable and non-culturable population was also detected. Species such as Talaromyces gossypii, Syncephalastrum monosporum, Aspergillus tabacinus, and Talaromyces verruculosus had remarkable degradation rates, up to 80% of diclofenac and bisphenol A was fully degraded. These results highlight the importance of characterizing autochthonous microorganisms and the possibility of selecting native fungal microorganisms to develop tailored biotransformation technologies for EPs.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the Potential of Sewage Sludge Mycobiome to Degrade High Diclofenac and Bisphenol-A Concentrations

Conejo-Saucedo

Ledezma-Villanueva

Paz

et al. 2021

Toxics

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“…synthetized dioxygenases or monooxygenases which was able to degrade PAHs. It is known that bacterial dioxygenases can catalyze the incorporation of both atoms of oxygen and two electrons from NADH to form cisdihydrodiol, while monooxygenases can hydrolyze PAHs into trans-dihydrodiols and this activity is slower than dioxygenases (Dudhagara and Dave 2018). In addition, some researchers suggested that the particular cell wall layer of mycobacteria, such as mycolic acids, is also important for the degradation of hydrophobic compounds (Zeng et al 2010).…”

Section: Taxonomic Composition Analysismentioning

confidence: 99%

Rhizosphere effect on removal and bioavailability of PAHs in contaminated agricultural soil

Możdżeń

Zhang

et al. 2021

Biologia

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The rhizosphere effect of ryegrass (Lolium perenne L.) on polycyclic aromatic hydrocarbons (PAHs) dissipation, bioavailability and the structure change of microbial community was investigated using a compartmented device-rhizobox. The PAHs removal efficiency, bioavailability and the change in structure of the microbial community were ascertained using HPLC, Tenax-TA extraction and PCR-DGGE, respectively. The results showed that in the root area (R1) and bulk soil (CK), the removal of 3-ring PAHs were 97.72 ± 0.34% and 95.51 ± 0.75%, 4-ring PAHs were 89.01 ± 1.61% and 78.65 ± 0.47%, 5-ring PAHs were 77.64 ± 4.05% and 48.63 ± 3.19%, 6-ring PAHs were 68.69 ± 3.68% and 36.09 ± 1.78%, respectively. The average removal efficiency of the total PAHs after 80 days followed the order: R1M (91.1%) > CKM (84.9%) > CK (77.6%), indicating that planted soil with inoculation of Mycobacterium sp. as well as non-planted soil inoculated with Mycobacterium sp. could both significantly accelerate the removal of PAHs compared to control soil. The bioavailability ratio of PAHs with 3 and 4 rings tended to decrease (from 59.9% to 14.8% for 3-ring and 7.61% to 5.08% for 4-ring, respectively in R1) while those with 5 rings increased significantly (from 2.41% to 33.78% in R1) during the last 40 days, indicating that bioavailability alteration varies with the number of rings in the PAHs. In addition, PAH bioavailability generally did not show a significant difference between treated soil and control soil. These results suggest that ryegrass rhizosphere effect as well as inoculation of Mycobacterium sp. can accelerate PAH removal in polluted soil. The bacteria community structure demonstrated a complex interplay of soil, bacteria and ryegrass root, and potential PAH degraders were present in abundance. This study provides the exploring data of rhizosphere and bioaugmentation effect on PAH dissipation in agricultural soil, as well as the change of bioavailability and microbial composition thereof.

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“…Nowadays, in order to eliminate PAHs from the environment by bioremediation, many PAHdegradation microorganisms have been isolated. Most of these bacteria belong to the genera Pseudomonas, Rhodococcus, Paenibacillus, Acinetobacter, Bacillus and Mycobacterium [15,16,2,17]. The aerobic and anaerobic bacteria species have been reported extensively in the literature for the degradation of low molecular weight (LMW) and high molecular weight (HMW) PAHs through pure cultures, consortia and mixed bacterial culture approaches (Patel et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Anthracene Utilizing Proteus vulgaris from Oil Spill Contaminated Soil at NNPC Depot Kano State Nigeria

Shehu

Ahmad

Yusuf

et al. 2021

JABB

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Microbial biodegradation of polycyclic aromatic hydrocarbons (PAHs) like Anthracene, represent an efficient, time, and cost-effective way for bioremediation of the polluted environment. This study was aimed at isolating, identifying and characterizing bacteria with potential to degrade and utilize anthracene as a sole carbon source. A bacteria was isolated from oil spilled contaminated site located in Kano, using an enrichment method on mineral salt media (MSM) following serial dilution (10-1-10-6). Characterization was done by studying the effects of temperature and pH on mineral salt media (MSM) containing anthracene. The isolate was then identified morphologically, biochemically and molecularly based on 16S rRNA partial gene sequence analysis. The morphological and microscopic examination of the isolate from this research shows that the isolate was creamy in color, motile, gram negative, short rod and non-spore forming respectively. The biochemical test of the isolate was found to be positive for these parameters (methyl red, catalase, motility, indole and urease) and negative for (citrate and oxidase). The 16S rRNA sequence and Phylogenetic analysis using neighbor joining tree and 1000 boos trap revealed that, the isolate was closely related (on the same clade) to Proteus vulgaris with accession number MW766369. Characterization was done by studying the effects of temperature and pH. The isolated bacterium was optimal at a temperature of 35ºC and pH 7.5. This isolate is a promising strain that could be used in bioremediation of polycylic aromatic hydrocarbons polluted environment.

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Mycobacterium as Polycyclic Aromatic Hydrocarbons (PAHs) Degrader

Cited by 12 publications

References 41 publications

Evaluation of the Potential of Sewage Sludge Mycobiome to Degrade High Diclofenac and Bisphenol-A Concentrations

Evaluation of the Potential of Sewage Sludge Mycobiome to Degrade High Diclofenac and Bisphenol-A Concentrations

Rhizosphere effect on removal and bioavailability of PAHs in contaminated agricultural soil

Isolation and Identification of Anthracene Utilizing Proteus vulgaris from Oil Spill Contaminated Soil at NNPC Depot Kano State Nigeria

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