Mycobacteriophage-drived diversification of Mycobacterium abscessus

Sassi, Mohamed; Gouret, Philippe; Chabrol, Olivier; Pontarotti, Pierre; Drancourt, Michel

doi:10.1186/1745-6150-9-19

Cited by 16 publications

(15 citation statements)

References 50 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The impact of the accessory genome on the evolution of the M. abscessus complex has been discussed by other researchers ( Choo et al, 2014 ; Sassi et al, 2014 ). In this analysis, we focused on the evolutionary pressure within the core genomes of the complex.…”

Section: Discussionmentioning

confidence: 95%

“…Recombination, an important evolutionary force for the diversification of bacterial core genomes ( Ehrlich et al, 2005 ; Redfield et al, 2006 ), has been reported in the M. abscessus complex ( Sassi et al, 2014 ). Gene acquisition at the hypothetical common ancestor apparently played an important role in the evolution of this species complex and the enhanced virulence in some members could have come from their ability to acquire genes from different bacterial genera via horizontal transfer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic Comparisons Reveal Microevolutionary Differences in Mycobacterium abscessus Subspecies

Tan

Ong

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

Mycobacterium abscessus, a rapid-growing non-tuberculous mycobacterium, has been the cause of sporadic and outbreak infections world-wide. The subspecies in M. abscessus complex (M. abscessus, M. massiliense, and M. bolletii) are associated with different biologic and pathogenic characteristics and are known to be among the most frequently isolated opportunistic pathogens from clinical material. To date, the evolutionary forces that could have contributed to these biological and clinical differences are still unclear. We compared genome data from 243 M. abscessus strains downloaded from the NCBI ftp Refseq database to understand how the microevolutionary processes of homologous recombination and positive selection influenced the diversification of the M. abscessus complex at the subspecies level. The three subspecies are clearly separated in the Minimum Spanning Tree. Their MUMi-based genomic distances support the separation of M. massiliense and M. bolletii into two subspecies. Maximum Likelihood analysis through dN/dS (the ratio of number of non-synonymous substitutions per non-synonymous site, to the number of synonymous substitutions per synonymous site) identified distinct genes in each subspecies that could have been affected by positive selection during evolution. The results of genome-wide alignment based on concatenated locally-collinear blocks suggest that (a) recombination has affected the M. abscessus complex more than mutation and positive selection; (b) recombination occurred more frequently in M. massiliense than in the other two subspecies; and (c) the recombined segments in the three subspecies have come from different intra-species and inter-species origins. The results lead to the identification of possible gene sets that could have been responsible for the subspecies-specific features and suggest independent evolution among the three subspecies, with recombination playing a more significant role than positive selection in the diversification among members in this complex.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Genomic Comparisons Reveal Microevolutionary Differences in Mycobacterium abscessus Subspecies

Tan

Ong

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…These prophages may play a major role in the evolution of STB, as previously reported for M . abscessus ( 27 ). Further study found that some STB isolates lacked the insertion element IS 1081, while a new ISMycA1 (GenBank accession number AJ619854) was discovered in the “ M. canettii ” CIPT140010059 genome ( 12 ).…”

Section: Particularities Of the Stb Organismsmentioning

confidence: 99%

Smooth Tubercle Bacilli: Neglected Opportunistic Tropical Pathogens

Osman

Bouzid

Canaan

et al. 2016

Front. Public Health

Self Cite

View full text Add to dashboard Cite

Smooth tubercle bacilli (STB) including “Mycobacterium canettii” are members of the Mycobacterium tuberculosis complex (MTBC), which cause non-contagious tuberculosis in human. This group comprises <100 isolates characterized by smooth colonies and cordless organisms. Most STB isolates have been obtained from patients exposed to the Republic of Djibouti but seven isolates, including the three seminal ones obtained by Georges Canetti between 1968 and 1970, were recovered from patients in France, Madagascar, Sub-Sahara East Africa, and French Polynesia. STB form a genetically heterogeneous group of MTBC organisms with large 4.48 ± 0.05 Mb genomes, which may link Mycobacterium kansasii to MTBC organisms. Lack of inter-human transmission suggested a yet unknown environmental reservoir. Clinical data indicate a respiratory tract route of contamination and the digestive tract as an alternative route of contamination. Further epidemiological and clinical studies are warranted to elucidate areas of uncertainty regarding these unusual mycobacteria and the tuberculosis they cause.

show abstract

“…Recently, the emergence of multidrug-resistant (MDR-TB) and extensively drug-resistant (XDR-TB) strains of M. tuberculosis has become a major global health concern (Cully M, 2014). The demand for new ways to overcome TB drug resistance has stimulated fresh research on mycobacteriophages and their lytic effi ciency against their hosts (Oldfi eld L M, et al, 2014;Sassi M, et al, 2014).…”

Section: Dear Editormentioning

confidence: 99%