Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig

Teng, Tieshan; Yu, Junping; Yang, Hang; Wei, Hongping

doi:10.1007/s12250-014-3545-4

Cited by 2 publications

(1 citation statement)

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“…Genetic analyses suggest that the bacteriophage genome is organized into functional modules. This modular organization allows genes that are involved in the same biological process to be clustered in the same module, which is common in most tailed bacteriophages ( Haddad et al, 2014 ; Teng et al, 2015 ). Furthermore, the phage does not have cohesive ends.…”

Section: Discussionmentioning

confidence: 99%

Characterization of novel bacteriophage phiC119 capable of lysing multidrug-resistant Shiga toxin-producingEscherichia coliO157:H7

Amarillas

Cháidez

González‐Robles

et al. 2016

PeerJ

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BackgroundShiga toxin-producing Escherichia coli (STEC) is one of the most common and widely distributed foodborne pathogens that has been frequently implicated in gastrointestinal and urinary tract infections. Moreover, high rates of multiple antibiotic-resistant E. coli strains have been reported worldwide. Due to the emergence of antibiotic-resistant strains, bacteriophages are considered an attractive alternative to biocontrol pathogenic bacteria. Characterization is a preliminary step towards designing a phage for biocontrol.MethodsIn this study, we describe the characterization of a bacteriophage designated phiC119, which can infect and lyse several multidrug-resistant STEC strains and some Salmonella strains. The phage genome was screened to detect the stx-genes using PCR, morphological analysis, host range was determined, and genome sequencing were carried out, as well as an analysis of the cohesive ends and identification of the type of genetic material through enzymatic digestion of the genome.ResultsAnalysis of the bacteriophage particles by transmission electron microscopy showed that it had an icosahedral head and a long tail, characteristic of the family Siphoviridae. The phage exhibits broad host range against multidrug-resistant and highly virulent E. coli isolates. One-step growth experiments revealed that the phiC119 phage presented a large burst size (210 PFU/cell) and a latent period of 20 min. Based on genomic analysis, the phage contains a linear double-stranded DNA genome with a size of 47,319 bp. The phage encodes 75 putative proteins, but lysogeny and virulence genes were not found in the phiC119 genome.ConclusionThese results suggest that phage phiC119 may be a good biological control agent. However, further studies are required to ensure its control of STEC and to confirm the safety of phage use.

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Section: Discussionmentioning

confidence: 99%