Mycobacterial infection of pigs in Croatia

Cvetnić, Željko; Špičić, Silvio; Benić, Miroslav; Katalinić-Janković, Vera; Pate, Mateja; Krt, B.; Ocepek, M.

doi:10.1556/avet.55.2007.1.1

Cited by 20 publications

(24 citation statements)

References 12 publications

(12 reference statements)

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“…Intradermal tuberculin testing can be used for ante mortem diagnosis of tuberculosis in pigs [4,9]. The single intradermal comparative test entails simultaneous injection of bovine and avian tuberculin.…”

Section: Introductionmentioning

confidence: 99%

Investigation of an outbreak of mycobacteriosis in pigs

Agdestein

Johansen

Polaček³

et al. 2011

BMC Vet Res

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BackgroundA high proportion of pigs imported to Serbia from a Lithuanian breeding herd reacted positively against avian and/or bovine tuberculin. The pigs were euthanized and lesions characteristic for mycobacterial infection were detected. An investigation of potential mycobacteriosis in the pigs imported to Serbia and the possible source of infection in the Lithuanian herd were therefore initialised.ResultsFormalin fixed, paraffin embedded lymph nodes from tuberculin positive animals were examined by real-time PCR for IS1245 and IS6110. IS1245 was detected in 55% and IS6110 in 11% of the samples. Seven of the ten IS6110 positive samples were positive for IS1245. Eleven lymph nodes from 10 pigs and 15 environmental samples were collected from the Lithuanian breeding herd and cultured for mycobacteria. M. avium subsp. hominissuis was detected in all lymph nodes and from eight samples of peat and sawdust. Isolates with identical and related IS1245- and IS1311 RFLP profiles were detected from swine and peat.ConclusionsThis study demonstrated cross reactions between avian and bovine tuberculin in pigs. Real-time PCR indicated infection with M. avium in the Serbian pigs. However, as a small proportion of the lymph nodes were positive for IS6110, infection with bacteria in the M. tuberculosis complex could not be ruled out. Analyses confirmed the presence of M. avium subsp. hominissuis in porcine and environmental samples from the Lithuanian breeding herd. The results indicate peat as a source of M. avium subsp. hominissuis infection in these pigs, and that the pigs imported to Serbia were infected with M. avium subsp. hominissuis.

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Section: Introductionmentioning

confidence: 99%

Investigation of an outbreak of mycobacteriosis in pigs

Agdestein

Johansen

Polaček³

et al. 2011

BMC Vet Res

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“…The prevalence of bacteriologically confirmed bovine tuberculosis in human patients was only 0.02% during that period (Cvetnic et al, 2000a). Human cases of the infection in regions free of bovine tuberculosis, including central European countries, are usually the result of reactivation of previous infections in elderly people Thoen et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Later investigations in pigs found infections caused especially by members of M. avium complex (MAC) and opportunistic mycobacteria i.e. M. fortuitum and M. chelonae (Cvetnic et al, 1998(Cvetnic et al, , 2006Matlova et al, 2005). In 1992 bovine tuberculosis was detected in wild boar in Croatia (Machackova et al, 2003).…”

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Mycobacterium caprae infection in cattle and pigs on one family farm inCroatia: a case report

Cvetnić¹,

Špičić²,

Katalinić-Janković³

et al. 2006

Vet. Med. - Czech

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An outbreak of tuberculosis among bovines and pigs caused by Mycobacterium caprae is described in this paper. After tuberculin skin tests with bovine purified protein derivates (PPD) six cattle and one sow, own by a small family farm, tested positive whilst three pigs were suspected in 2004. All animals were euthanised and checked for gross pathological lesions. Generalised lesions were found in five cattle and two sows; however one calf and two gilts had lesions that were localised in the submandibular lymph nodes. Mycobacteria were isolated from tissue samples of six cattle and four pigs. Mycobacterial isolates were identified using classical biochemical tests and molecular methods (PCR, GenoType MTBC) as M. caprae. Mycobacterial Interspersed Repetitive Unit (MIRU) typing of isolated mycobacteria showed an identical number of repeats in 12 different loci. Results of the research confirmed the domination of M. caprae among infected cattle in Croatia; however this paper was the first to confirm a case of M. caprae in pigs. The source of the infection was not found.

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“…Isolates were typed by conventional methods, PCR and hybridization. Most of the isolates belonged to the Mycobacterium avium complex (95.7%), of these strains M. a. hominissuis accounted for 78.9% and M. a. avium for the rest of isolates [4]. M. avium subsp.…”

Section: Introductionmentioning

confidence: 99%

Diarrhea and Colonic Ulcers of Unusual Etiology

Keynan¹,

Cantor²,

Grynspan³

et al. 2013

Mycobact Diseases

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The present report describes a case of chronic diarrhea with several ulcers in the ascending colon in a 47-yearold woman who immigrated to Canada from the Phillipines. A biopsy from affected colon revealed caseating granulomas and culture grew mycobacterium avium subspecies. Polymerase chain reaction from the colonic biopsy specimen confirmed the presence of mycobacterium avium subspecies that was subsequently identified by hsp65 sequence to be mycobacterium avium hominissuis (MAH). Anti-mycobacterial therapy was started but discontinued by the patient. After 6 months a repeat colonoscopy was macroscopically normal and no granulomas could be seen on biopsies taken from normal appearing colonic tissue. The epidemiological features, host-range and laboratory identification of this unusual organism are discussed.

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Mycobacterial infection of pigs in Croatia

Cited by 20 publications

References 12 publications

Investigation of an outbreak of mycobacteriosis in pigs

Investigation of an outbreak of mycobacteriosis in pigs

Mycobacterium caprae infection in cattle and pigs on one family farm inCroatia: a case report

Diarrhea and Colonic Ulcers of Unusual Etiology

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