Mycobacterial glycoconjugates as vaccine candidates against tuberculosis

Källenius, Gunilla; Pawłowski, Andrzej; Hamasur, Beston; Svenson, Stefan B.

doi:10.1016/j.tim.2008.07.007

Cited by 42 publications

(40 citation statements)

References 75 publications

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“…2, 3 The glycosylation or other controlled chemical modications of proteins, such as PEGylation and acylation, can dramatically improve their physical and biological properties. 4 For instance, chemical conjugation with suitable glycans has been exploited for the production of therapeutic enzymes with improved activity and reduced in vivo metabolism.…”

Section: Introductionmentioning

confidence: 99%

Chemoenzymatic synthesis of neoglycoproteins driven by the assessment of protein surface reactivity

et al. 2014

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In this paper a series of 2-iminomethoxyethyl mannose-based mono-and disaccharides have been synthesized by a chemoenzymatic approach and used in coupling reactions with 3-amino groups of lysine residues in a model protein (ribonuclease A, RNase A) to give semisynthetic neoglycoconjugates. In order to study the influence of structure of the glycans on the conjugation outcomes, an accurate characterization of the prepared neoglycoproteins was performed by a combination of ESI-MS and LC-MS analytical methods. The analyses of the chymotryptic digests of the all neoglycoconjugates revealed six Lys-glycosylation sites with a the following order of lysine reactivity: Lys 1 [ Lys 91 y Lys 31 > Lys 61 y Lys 66. A computational analysis of the reactivity of each lysine residue has been also carried out considering several parameters (amino acids surface exposure and pK a , protein flexibility). The in silico evaluation seems to confirm the order in lysine reactivity resulting from proteomic analysis.

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Section: Introductionmentioning

confidence: 99%

Chemoenzymatic synthesis of neoglycoproteins driven by the assessment of protein surface reactivity

et al. 2014

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“…They play a role in biological recognition phenomena involving cells and proteins. Moreover, oligosaccharides are present in the form of lectins in all cell walls mediating a variety of events such as inflammation, cell-cell recognition, immunological response, metastasis, and fertilization (31,32). In the food industry, calcium binding marine food-derived lectins can be incorporated in nutraceutical or functional food to prevent calcium deficiency.…”

Section: Potential Functional Ingredients From Marine Productsmentioning

confidence: 99%

Application of marine biomaterials for nutraceuticals and functional foods

Zhang

Kim

2012

Food Sci Biotechnol

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Recently, a great deal of interest has been paid by the consumers towards natural bioactive compounds as functional ingredients in the diets due to their various health beneficial effects. Hence, it can be suggested that bio-processed marine products are alternative sources for synthetic ingredients that can contribute to consumer's well-being, as a part of nutraceuticals and functional foods. The ultra filtration membrane bioreactor is a novel technology to bio-process marine products. This review presents an overview of bio-processing and perspectives of bioprocessed marine products for use as nutraceuticals and functional foods.

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“…In preclinical animal experiments they proved to be highly efficient in protection against experimental challenge with virulent Mtb, both in terms of reduced pathology and long time survival which was equal to or slightly better than that conferred by conventional BCG vaccination. 48,63 Of particular interest is the finding that the AM oligosaccharde-tetanus toxoid (the heterologous carrier protein unrelated to Mtb) conjugate vaccine afforded significant protection. This provided the proof of principle that immunity elicited by a carbohydrate antigen alone can confer protective immunity against TB Hence, mycobacterial carbohydrates are important candidate antigens which should be included in a forthcoming new vaccine.…”

Section: Mycobacterial Carbohydrate Antigen-based Vaccine Candidatesmentioning

confidence: 99%