Myco-nanocolloids manipulate growth, biofilm formation and virulence genes in UTI causing
            <i>E. coli</i>

Ranjani, S; Das, Rumelee; M, Shariq Ahmed; Lalnunmawii, Esther; Senthilkumar, N.; Ruckmani, K.; Hemalatha, S.

doi:10.1080/24701556.2020.1852426

Cited by 14 publications

(4 citation statements)

References 28 publications

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“…Equal volume of endophytic fungal extract and 1 mM AgNO 3 solution was mixed with gentle swirling and incubated in dark for 48h at room temperature. The colour of the solution changed from colourless to brown con rmed the synthesis of myconanoparticles (12)(13)(14)(15)(16)18).…”

Section: Biosynthesis Of Myconnaoparticles Loaded With Metabolites Of Endophytic Fungus Penicillium Sclerotiorummentioning

confidence: 97%

“…Leaves were cut into small pieces (1mm) and surface sterilized with distilled water and followed by sequential rinsing in 70% ethanol for 1 min, 1% Sodium hypochlorite for 1min, 90% ethanol for 5 min, nally rinsed with sterile distilled water for 2-3times and then allowed to dry under sterile conditions in laminar air ow. The cut surface of the segments were placed in petri dishes containing PDA supplemented with Ampicillin (1mg/lit) in a laminar air ow chamber and incubated at endophytic fungi were growing out from the explants was sub-cultured in new PDA plates to obtain pure culture (12)(13)(14)(15).…”

Section: Isolation and Identi Cation Of Endophytic Fungi From Tamarindus Indicamentioning

confidence: 99%

“…Polymerase chain reaction was performed as prescribed previously (17). The ampli ed PCR product was used for sequencing and further utilized for construction of phylogenetic tree using MEGA-X, based on Maximum Likelihood algorithm and, endophytic fungal sequence was submitted to NCBI Genbank (12)(13)(14)(15).…”

Section: Isolation and Identi Cation Of Endophytic Fungi From Tamarindus Indicamentioning

confidence: 99%

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Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Ranjani

Kathun

Hemalatha

2021

Appl Biochem Biotechnol

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Nanotechnology involves the synthesis of nanoparticles that has been used in therapeutic application for treating diseases. In this present study we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated for its antibacterial potential against antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Endophytic fungi were isolated from the healthy leaves of Tamarindus indica. The genomic DNA from endophytic fungi was isolated and ITS region was ampli ed by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4and sequenced for the identi cation of endophytic fungal isolates. Penicillium sclerotiorum extract were used for the synthesis silver nanoparticles (PsNps) and were characterized by UV-Vis spectroscopy, Fourier transform-infrared spectroscopy (FTIR), Zeta potential, FE-SEM and Energy-Dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, bio lm formation in both the strains of E. coli. The expression of gene encoding CTX-M-15 was down regulated in resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for the antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.

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Section: Biosynthesis Of Myconnaoparticles Loaded With Metabolites Of Endophytic Fungus Penicillium Sclerotiorummentioning

confidence: 97%

Section: Isolation and Identi Cation Of Endophytic Fungi From Tamarindus Indicamentioning

confidence: 99%

Section: Isolation and Identi Cation Of Endophytic Fungi From Tamarindus Indicamentioning

confidence: 99%

See 1 more Smart Citation

Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Ranjani

Kathun

Hemalatha

2021

Appl Biochem Biotechnol

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“…Plates were periodically observed for growth of endophytic fungi. After 7 days, endophytic fungi were growing out from the explants was sub-cultured in new PDA plates to obtain pure culture (12)(13)(14)(15).…”

Section: Isolation and Identi Cation Of Endophytic Fungi From Tamarindus Indicamentioning

confidence: 99%

Silver decorated myconanoparticles control growth and biofilm formation in uropathogenic E. coli

Ranjani

Hemalatha

2021

Preprint

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Nanotechnology involves the synthesis of nanoparticles that has been used in therapeutic application for treating diseases. In this present study we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated for its antibacterial potential against antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Endophytic fungi were isolated from the healthy leaves of Tamarindus indica. The genomic DNA from endophytic fungi was isolated and ITS region was amplified by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4and sequenced for the identification of endophytic fungal isolates. Penicillium sclerotiorum extract were used for the synthesis silver nanoparticles (PsNps) and were characterized by UV-Vis spectroscopy, Fourier transform- infrared spectroscopy (FTIR), Zeta potential, FE-SEM and Energy- Dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli. Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, biofilm formation in both the strains of E. coli. The expression of gene encoding CTX-M-15 was down regulated in resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for the antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.

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Engineered green nanoparticles interact withNigrospora oryzaeisolated from infected leaves ofArachis hypogaea

2022

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Oilseed crop diseases are a major concern around the world, particularly in India. The synthetic fungicides not only kill the pathogen, but they also harm the host plant and beneficial microbes and on continuous usage, they decrease the soil fertility. To overcome this problem, green nanotechnology has been a greater alternative with promising benefits. The green synthesized nanoparticles from the extract of various plant parts are an effective remedy for killing the pathogens without affecting the host plants and the environment. Hence, in our study silver nanoparticles were synthesized from Fennel seed (Foeniculum vulgare) extract. The synthesis of nanoparticles was confirmed using UV-vis, Fourier transform infrared, dynamic light scattering, zeta potential, and scanning electron microscopic analysis. The in vitro antifungal study was carried out and revealed that the nanoparticles had high efficacy against the isolated phytopathogen Nigrospora oryzae which causes tikka disease in Arachis hypogaea plants. Hence, F. vulgare seed nanoparticles can be used as an effective alternative to synthetic fungicides without causing any deleterious effect on soil microflora or the environment.

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Myco-nanocolloids manipulate growth, biofilm formation and virulence genes in UTI causing E. coli

Cited by 14 publications

References 28 publications

Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

Silver decorated myconanoparticles control growth and biofilm formation in uropathogenic E. coli

Engineered green nanoparticles interact withNigrospora oryzaeisolated from infected leaves ofArachis hypogaea

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