MYC‐activated lncRNA HNF1A‐AS1 overexpression facilitates glioma progression via cooperating with miR‐32‐5p/SOX4 axis

Abstract

Mounting literatures have revealed the crucial effects of long noncoding RNA (lncRNA) in various cancers, including glioma. HNF1A‐AS1, a novel lncRNA, is reported to modulate tumorigenesis and development of multiple cancers. However, the tumorigenic function of lncRNA HNF1A‐AS1 in glioma remains largely unknown. quantitative reverse transcription and polymerase chain reaction and western blot assays were applied to evaluate the expression of relevant mRNAs and proteins. 5‐Ethynyl‐2’‐ deoxyuridine, terminal deoxynucleotidyl transferase dUTP nick‐end labeling, flow cytometry, and transwell assays were conducted for examining the influence of HNF1A‐AS1 on glioma cell functions. The relationship among RNAs was investigated by mechanical experiments. The results demonstrated that HNF1A‐AS1 was predominantly highly expressed in glioma cell lines compared with nontumor glial epithelial cell, which was associated with the stimulation of transcription factor myelocytomatosis oncogene. Knockdown of HNF1A‐AS1 remarkably inhibited glioma cells proliferation, migration, and invasion, while accelerating cell apoptosis in vitro. Mechanically, HNF1A‐AS1 served as a miR‐32‐5p sponge. Moreover, SOX4 was discovered as a target of miR‐32‐5p. Inhibited miR‐32‐5p or upregulated SOX4 could markedly counteract the inhibitory effects of silencing HNF1A‐AS1 on glioma malignant biological behaviors. HNF1A‐AS1 exerted oncogenic property in glioma progression via upregulating miR‐32‐5p–mediated SOX4 expression, suggesting potential novel therapeutic target for future glioma treatment.

Molecular mechanism of m6A methylation of circDLC1 mediated by RNA methyltransferase METTL3 in the malignant proliferation of glioma cells

“…The preliminary nucleus was resuspended in 3 mL 2 M RSB (2 M sucrose, 10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , 1 mM DTT, and 0.5 mM PMSF) and centrifuged at 3000 × g for 45 min. The isolated circDLC1 was subjected to RT-qPCR analysis, with U6 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as nuclear and cytoplasmic controls, respectively [ 31 ].…”

m6A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer via IGF2BP2-Mediated CCND1 mRNA Stabilization

“…MeRIP experiments revealed that after silencing METTL3, the m 6 A level of FAM225A decreased, along with the decreased stability and expression of FAM225A [14]. There have been studies about the pivotal role of HNF1A-AS1 in NSCLC, hepatocellular carcinoma and gastric cancer [15][16][17][18][19]. In CRC, studies have shown that HNF1A-AS1 mainly played biological roles through competitively interacting with microRNAs, such as miR-34a, miR-124 and so on [20,21].…”