MutS HOMOLOG1 silencing mediatesORF220substoichiometric shifting and causes male sterility inBrassica juncea

Abstract

Highlight MSH1 participates in fertility reversion and male sterility by mediating mitochondrial genomic substoichiometric shifting of ORF220 and altering expression of anther development-associated genes in Brassica juncea.

The role of mitochondria in plant development and stress tolerance

“…Moreover, altered activity of MSH1 in Brassica juncea is correlated to the substoichiometric shifting of the CMS-associated ORF220. Notably, increased ORF220 copy number was found in male sterile RNAi-MSH1 lines, which provides a new approach to manipulate CMS for agronomic use (ZHAO et al 2015).…”

MutS‐Homolog2 silencing generates tetraploid meiocytes in tomato (Solanum lycopersicum)

“…It is reported that the other members of MSH2 family function beyond MMR. MSH1 is required for mitochondrial stability (Reenan & Kolodner, ) and disruption of MSH1 can influence male sterility in several species (Sandhu, Abdelnoor, & Mackenzie, ; Zhao et al., ). The MSH4 and MSH5 form heterodimer between them without involving MSH2 and exclusively function in meiosis (Hollingsworth, Ponte, & Halsey, ; Lu et al., ; Schofield & Hsieh, ; Wang et al., ).…”

Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea

“…The orf108 gene is also associated with CMS in the cytoplasm of Moricandia arvensis ( Ashutosh et al , 2008 ; Kumar et al , 2012 ). A chimeric orf220 gene from CMS B. juncea (stem mustard) causes male sterility ( Yang et al , 2010 ) and MSH1-RNAi from spontaneous fertile-revertant lines was found to increase the orf220 copy number and induce male sterility ( Zhao et al , 2016 ).…”

“…A number of mitochondrial genes and those involved early anther development (e.g. WUS , SPL , AP3 , PI , DYT1 , and AMS ) were found to be down-regulated in male-sterility lines ( Yang et al , 2010 ; Zhao et al , 2016 ).…”