Mutations potentially associated with decreased susceptibility to fluoroquinolones, macrolides and lincomycin in Mycoplasma synoviae

Bekő, Katinka; Kreizinger, Zsuzsa; Kovács, Áron Botond; Sulyok, Kinga M.; Marton, Szilvia; Bányai, Krisztiàn; Catania, Salvatore; Feberwee, Anneke; Wiegel, Jeanine; Dijkman, Remco; Veen, C Ter; Lysnyansky, Inna; Gyuranecz, Miklós

doi:10.1016/j.vetmic.2020.108818

Cited by 5 publications

(9 citation statements)

References 31 publications

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“…Seven M. synoviae isolates showed an MIC > 32 μg/mL for TIL between 2013 and 2016 [ 25 ]. High MIC values were also detected in another study, which showed 25/87 M. synoviae strains with high MIC values (> 8 μg/ml for TIL and/or > 1 μg/ml for TY and/or > 0.5 μg/ml for tylvalosin) from 18 different countries from 1982 to 2019 [ 26 ]. As mentioned above, increased TIL MICs (≥64 μg/ml MIC 90 values) were also detected in M. synoviae isolates collected from China, India, Indonesia, Malaysia, the Philippines, the Republic of Korea, and Thailand [ 21 ].…”

Section: Discussionmentioning

confidence: 86%

“…All M. synoviae isolates remained sensitive to TIF with MICs ranging from 0.12 to 2.5 μg/mL in South Africa between 2003 and 2015 [ 16 ]. The MIC values of VA were ≤ 0.039 μg/mL in Central and Eastern Europe [ 26 ]. The M. synoviae isolates examined in this study showed high susceptibility to VA (MIC < 0.016 ~ 0.031 μg/mL) and TIF (MIC < 0.016 ~ 0.063 μg/mL).…”

Section: Discussionmentioning

confidence: 99%

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Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019

et al. 2022

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Background In the past decade, Mycoplasma synoviae (M. synoviae) infection has become widely prevalent in China, has caused serious economic losses and has become one of the most important diseases in the chicken industry. Medication is a general approach for the control of M. synoviae infection, but antibiotics are sometimes ineffective in clinical practice. To investigate the sensitivity of M. synoviae to antimicrobials commonly used in the treatment of M. synoviae infection, the antibiotic susceptibility of 32 M. synoviae strains isolated from China from 2016 to 2019 were determined using the minimum inhibitory concentration (MIC) method. Results All isolates had low MIC values for the combination of lincomycin and spectinomycin, pleuromutilin, and macrolides. However, the M. synoviae isolates displayed variance in MICs for doxycycline hydrochloride with a range of 0.25 to 8 μg/mL, and oxytetracycline hydrochloride with a range of 0.5 to 8 μg/mL. Three and one M. synoviae isolates showed intermediate MIC values to doxycycline hydrochloride and oxytetracycline hydrochloride, respectively. High MIC values for enrofloxacin were detected in all isolates with MICs ranging from 4 to 32 μg/mL. Furthermore, comparison of the parC QRDR identified a mutation at nucleotide position 254 (C254T) resulting in a Thr 85 Ile amino acid change in all M. synoviae isolates and the reference strain ATCC 25204 being resistant to enrofloxacin. Moreover, mutations at Glu 804 Gly and Thr 686 Ala of gyrA QRDR were identified in all M. synoviae isolates and ATCC 25204. The mutation in the QRDR of the parE gene resulted in amino acid changes at positions 197 (Pro to Ser) in 27/32 M. synoviae isolates. Conclusion Three nonsynonymous mutations in gyrA and parE were first identified to be related to enrofloxacin resistance. Our results showed that M. synoviae resistance to enrofloxacin is widespread.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019

et al. 2022

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“…The 23S rRNA and 50S ribosomal protein L4 and L22 coding genes were previously connected to macrolide and lincomycin resistance in Mycoplasma species (Kobayashi et al, 2005;Sulyok et al, 2017;Bek} o et al, 2020a;Felde et al, 2020). These genes were aligned and analysed in Geneious Prime software version 2019.2.1 (Kearse et al, 2012) to detect SNPs.…”

Section: Development Of the Assaymentioning

confidence: 99%

“…One of the allele-specific primers is marked with an additional 15-to 20-bp-long GC clamp that increases the melting temperature and the size of the amplicon as well. The temperature shift is detected on a real-time PCR platform in the presence of an intercalating fluorescent DNA dye (melt-MAMA) and the size difference is revealed in agarose gel electrophoresis (agarose-MAMA) (Birdsell et al, 2012;Sulyok et al, 2018;Bek} o et al, 2020b;Felde et al, 2020). Primers were designed based on the whole genome sequences of M. hyorhinis field isolates and the type strain (NCTC 10130) in the Geneious Prime software version 2019.2.1 (Kearse et al, 2012).…”

Section: Development Of the Assaymentioning

confidence: 99%

“…A point mutation A2059G (according to Escherichia coli numbering) at the V domain of the 23S rRNA was found in in vitro mutants and field isolates as well. In addition, point mutations between nucleotide positions 2058 and 2062 (E. coli numbering) were connected to macrolide and lincosamide resistance in several Mycoplasma species (Sulyok et al, 2017;Chernov et al, 2018;Gautier-Bouchardon, 2018;Bek} o et al, 2020a;Felde et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Development of a molecular biological assay for the detection of markers related to decreased susceptibility to macrolides and lincomycin in Mycoplasma hyorhinis

Földi

Kreizinger

Bekő

et al. 2021

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The control of Mycoplasma hyorhinis infection relies mainly on antimicrobial therapy. However, the antibiotic susceptibility testing of the bacteria is usually not performed before applying the treatment, and thus therapeutic failures are not uncommon. In the case of M. hyorhinis, several antibiotic-resistance-related single nucleotide polymorphisms (SNPs) are known but assays for their detection have not been described yet. The aims of the present study were to investigate macrolide- and lincomycin-resistance-related SNPs in Hungarian M. hyorhinis isolates and to develop mismatch amplification mutation assays (MAMA) to detect the identified resistance markers. Minimal inhibitory concentrations (MIC) of different drugs and whole genome sequences of 37 M. hyorhinis isolates were used to find the resistance-related mutations. One MAMA assay was designed to detect the mutation of the 23S rRNA gene at nucleotide position 2058 (Escherichia coli numbering). For further evaluation, the assay was challenged with 17 additional isolates with available MIC data and 15 DNA samples from clinical specimens. The genotypes of the samples were in line with the MIC test results. The developed assay supports the practice of targeted antibiotic usage; hence it may indirectly reduce some bacterial resistance-related public health concerns.

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Identification and detection of mutations potentially associated with decreased susceptibility to macrolides and lincomycin in Mycoplasma anserisalpingitidis isolates

Grózner

Bekő

Kovács

et al. 2022

Veterinary Microbiology

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Mutations potentially associated with decreased susceptibility to fluoroquinolones, macrolides and lincomycin in Mycoplasma synoviae

Cited by 5 publications

References 31 publications

Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019

Antibiotic resistance of Mycoplasma Synoviae strains isolated in China from 2016 to 2019

Development of a molecular biological assay for the detection of markers related to decreased susceptibility to macrolides and lincomycin in Mycoplasma hyorhinis

Identification and detection of mutations potentially associated with decreased susceptibility to macrolides and lincomycin in Mycoplasma anserisalpingitidis isolates

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