Mutations or exclusion: an unusual case in paternity testing

Abstract

In an immigration case with the scope of family reunification, the DNA extracted from the saliva samples of the male child, the alleged mother and the putative father was typed with 22 autosomal short tandem repeat (STR) systems. In seven STR systems, the alleged mother could be excluded from maternity, and the case then had to be regarded as a deficiency case. Taking this fact into consideration, only two exclusions were found for the putative father, and the question arose whether there was an exclusion of the putative father or the existence of two mutations. Autosomal STR typing could not clarify the case, but the application of eight Y-chromosomal markers showed that the alleged father could be excluded from paternity.

Genetic data of nine non-CODIS STRs in Chinese Han population from Guangdong Province, Southern China

“…In forensic casework, ordinary parentage cases can be expected to be solved by a commercially available multiplexes of STRs genotyping kit, e.g., PowerPlex 16 System Kit (Promega, Madison, WI, USA) or AmpFℓSTR Identifiler polymerase chain reaction (PCR) amplification kit (Applied Biosystems, Foster City, CA, USA). However, for more difficult cases, such as deficient cases [1], related individuals are involved [2,3], mutations are encountered [4], or complex kinship analysis [5,6] and common STRs cannot provide enough discriminatory power [5,7,8]. Additional STRs are needed to complement conventional analysis for obtain more information.…”

Population data of 21 non-CODIS STR loci in Han population of northern China

“…In some complex kinship analysis cases (e.g., distant relatives or with mutations [21]), current commercial kits with CODIS core STRs may not be able to provide high enough likelihood ratio or probability of paternity to obtain reliable identifications. More STRs can provide extra information to raise the confidence of identifications.…”

Reinvestigations of six unusual paternity cases by typing of autosomal single‐nucleotide polymorphisms

“…Blood samples from 12 individuals 12,13 were provided by Dr Martin R. Whittle (Genomic Engenharia Molecular, São Paulo, Brazil) and Dr Cornelius Courts and Prof. Dr Med. Burkhard Medea (Institut Für Rechtsmedizin, Bonn, Germany).…”