“…To exclude such a possibility, we chose cells that lack both wild-type APC alleles but show little CIN, that is, DLD-1 cells that are MIN cell line with near-diploid chromosome content, and show minimal CIN (Aoki et al, 2003;Stewenius et al, 2005). Notably, DLD-1 cells lack full-length APC, but express its C-terminally truncated protein that cannot bind to the kinetochore (Homfray et al, 1998;Fodde et al, 2001). In this cell line, the Wnt transcription is relatively lower than those in other cells with Wnt signal activation (van de Wetering et al, 2002).…”