Mutations in rpoB and katG genes in Mycobacterium isolates from the Southeast of Mexico

Zenteno-Cuevas, Roberto; Jc, Zenteno; Cuellar, Aremy; Cuevas, Beatriz; Sampieri, Clara Luz; Riviera, JE; Parissi, A

doi:10.1590/s0074-02762009000300012

Cited by 29 publications

(15 citation statements)

References 21 publications

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“…In our study, both susceptible and resistant, mainly MDR, M. tuberculosis strains were evaluated; unfortunately, only a limited number of strains resistant to second-line drugs were evaluated. We found the performance of the GenoType tests in Colombia to be similar to or even better than those described in studies elsewhere, like in Mexico, Brazil, Peru, Chile, Bolivia, France, Italy, Turkey, Spain, Vietnam, the Russian Federation, Ethiopia, and China (4,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). In general, sensitivity, specificity, positive and negative predictive values, and concordance with phenotypic DST support the utility of these tests for the screening of resistance to first-and second-line antituberculosis drugs in our country.…”

Section: Discussionsupporting

confidence: 82%

“…Specifically, the frequency of the katG MUT S315T1 in Latin America varied, with 52% in Mexico, 61.9% in Brazil, 71.2% in Peru, and 88% in Colombia (12)(13)(14). In comparison, the frequency in some countries on other continents was 65% in France, 66.5% in Italy, 73% in Turkey, 87% in Spain, 88% in Vietnam, 94% in the Russian Federation, and 94% in Ethiopia (17)(18)(19)(20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

“…56.4% in Peru, 56% in Chile, 59% in Bolivia, and 64% in Colombia (12,(14)(15)(16); in other countries not in the Latin American region, the percentage seems to be higher, with 47% in France, 53.4% in Turkey, 58.5% in Italy, 60% in Vietnam, 63% in Spain, 73% in Ethiopia, and 86.9% in the Russian Federation (17)(18)(19)(20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

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Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

et al. 2013

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Previous evaluations of the molecular GenoType tests have promoted their use to detect resistance to first-and second-line antituberculosis drugs in different geographical regions. However, there are known geographic variations in the mutations associated with drug resistance in Mycobacterium tuberculosis, and especially in South America, there is a paucity of information regarding the frequencies and types of mutations associated with resistance to first-and second-line antituberculosis drugs. We therefore evaluated the performance of the GenoType kits in this region by testing 228 M. tuberculosis isolates in Colombia, including 134 resistant and 94 pansusceptible strains. Overall, the sensitivity and specificity of the GenoType MTBDRplus test ranged from 92 to 96% and 97 to 100%, respectively; the agreement index was optimal (Cohen's kappa, >0.8). The sensitivity of the GenoType MTBDRsl test ranged from 84 to 100% and the specificity from 88 to 100%. The most common mutations were katG S315T1, rpoB S531L, embB M306V, gyrA D94G, and rrs A1401G. Our results reflect the utility of the GenoType tests in Colombia; however, as some discordance still exists between the conventional and molecular approaches in resistance testing, we adhere to the recommendation that the GenoType tests serve as early guides for therapy, followed by phenotypic drug susceptibility testing for all cases.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

et al. 2013

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“…This is noteworthy as mutations of these codons are rare compared to other mutation hotspots like codons 526 and 531 [29–32]. Even more astounding is the absence of the WT4 hybridization signal combined with the presence of both WT3 and WT5, observed in 2/5 strains, as all codons that are covered by WT4 are also covered by either WT3 or WT5.…”

Section: Discussionmentioning

confidence: 97%

A Systematic Follow-Up ofMycobacterium tuberculosisDrug-Resistance and Associated Genotypic Lineages in the French Departments of the Americas over a Seventeen-Year Period

Millet

Streit

Berchel

et al. 2014

BioMed Research International

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The population of the French Departments of the Americas (FDA) is highly influenced by the intense migratory flows with mainland France and surrounding countries of the Caribbean and Latin America, some of which have high incidence rates of tuberculosis (Haiti: 230/100,000; Guyana: 111/100,000; and Suriname: 145/100,000) and drug resistance. Since the development of drug resistance to conventional antituberculous drugs has a major impact on the treatment success of tuberculosis, we therefore decided to review carefully Mycobacterium tuberculosis drug resistance and associated genotypic lineages in the FDA over a seventeen-year period (January 1995–December 2011). A total of 1239 cases were studied, including 153 drug-resistant and 26 multidrug-resistant- (MDR-) TB cases, representing 12.3% and 2.1% of the TB cases in our study setting. A significantly higher proportion of M. tuberculosis isolates among relapse cases showed drug resistance to isoniazid (22.5%, P = 0.002), rifampicin (20.0%, P < 0.001), or both (MDR-TB, 17.5%; P < 0.001). Determination of spoligotyping based phylogenetic clades showed that among the five major lineages observed—T family (30.1%); Latin-American and Mediterranean (LAM, 23.7%); Haarlem (H, 22.2%); East-African Indian (EAI, 7.2%); and X family (6.5%)—two lineages, X and LAM, were overrepresented in drug-resistant and MDR-TB cases, respectively. Finally, 19 predominant spoligotypes were identified for the 1239 isolates of M. tuberculosis in our study among which 4 were significantly associated with drug resistance corresponding to SIT20/LAM1, SIT64/LAM6, SIT45/H1, and SIT46/undefined lineage.

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“…For example, 52% INH-resistant TB isolates from Mexico [21], 69% of the isolates from Poland [22], 72% from Brazil [23], 100% from Kazakhstan [24], and 53% from the North of Mexico had KatG mutation [25]. In partial agreement with our results, Nusrath Unissa et al (2008) from India [26], Torres (2002) from Spain [27], and Ramaswamy (2004) from Mexico [28] reported KatG mutation in 64, 62 and 67% of isolates, respectively.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors of drug-resistant tuberculosis in border provinces of Iran

Sahebi¹,

Ansarin²,

Farajnia³

et al. 2015

Postgraduate Medicine

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Mutations in rpoB and katG genes in Mycobacterium isolates from the Southeast of Mexico

Abstract: The most frequent mutations associated with rifampin and isoniazid resistance in

Cited by 29 publications

References 21 publications

Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

A Systematic Follow-Up ofMycobacterium tuberculosisDrug-Resistance and Associated Genotypic Lineages in the French Departments of the Americas over a Seventeen-Year Period

Prevalence and risk factors of drug-resistant tuberculosis in border provinces of Iran

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