Mutations and amplification of EPSPS gene confer resistance to glyphosate in goosegrass (Eleusine indica)

Chen, Jingchao; Huang, Hongjuan; Zhang, Chaoxian; Wei, Shugang; Huang, Zhengxu; Chen, Jinyi; Wang, Xu

doi:10.1007/s00425-015-2324-2

Cited by 93 publications

(124 citation statements)

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“…This suggests that their resistance mechanism did not involve the target-site. Similar results were reported in resistant populations of E. indica (population R3) and E. colona (population RLB2) (Alarcón-Reverte et al, 2015; Chen et al, 2015), in which no important differences in their inhibition of the EPSPS activity with respect to their sensitive populations were reported.…”

Section: Discussionsupporting

confidence: 87%

“…Other glyphosate-resistant grass weeds such as: Bromus diandrus, Echinochloa colona, Eleusine indica, Lolium perenne spp. multiflorum, L. rigidum, Poa annua , among others (González-Torralva et al, 2012; Alarcón-Reverte et al, 2015; Chen et al, 2015; Cross et al, 2015; Fernandez et al, 2015; Salas et al, 2015; Yu et al, 2015; Malone et al, 2016), exhibited RI values that ranged between 3 to 19, and between 4 to <182 based on in their ED 50 or LD 50 , respectively. Differences in the level of glyphosate resistance between these species were due to various resistance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…A. tuberculatus, B. diandrus, B. pilosa, D. insularis, E. colona, E. indica, L. perenne sp. multiflorum, L. rigidum, K. scoparia (Bostamam et al, 2012; de Carvalho et al, 2012; González-Torralva et al, 2012; Alarcón-Reverte et al, 2015; Chatham et al, 2015; Chen et al, 2015; Fernandez et al, 2015; Salas et al, 2015; Wiersma et al, 2015; Alcántara-de la Cruz et al, 2016b; Malone et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

“…Both groups limiting the amount of glyphosate which reaches the EPSPS at toxic levels, or causing a loss of affinity between the EPSPS and the glyphosate (Powles, 2010). Target-site resistance (TSR) mechanisms includes amino acid substitutions in DNA sequence, represented by nucleotide changes in Thr-102 and Pro-106 positions, that synthesizes the EPSPS enzyme (Sammons and Gaines, 2014; Chen et al, 2015; Yu et al, 2015; Alcántara-de la Cruz et al, 2016b); multiple EPSPS gene copy numbers resulting in a higher amplification, expression and activity of the EPSPS (Gaines et al, 2013; Vila-Aiub et al, 2014). To date, glyphosate is the only herbicide known to evolve amplification of the target-site (EPSPS) conferring resistance with field applications (Ribeiro et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

et al. 2016

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Leptochloa virgata (L.) P. Beauv. is an annual weed common in citrus groves in the states of Puebla and Veracruz, Mexico limiting their production. Since 2010, several L. virgata populations were identified as being resistant to glyphosate, but studies of their resistance mechanisms developed by this species have been conducted. In this work, three glyphosate-resistant populations (R8, R14, and R15) collected in citrus orchards from Mexico, were used to study their resistance mechanisms comparing them to one susceptible population (S). Dose-response and shikimic acid accumulation assays confirmed the glyphosate resistance of the three resistant populations. Higher doses of up to 720 g ae ha-1 (field dose) were needed to control by 50% plants of resistant populations. The S population absorbed between 7 and 13% more 14C-glyphosate than resistant ones, and translocated up to 32.2% of 14C-glyphosate to the roots at 96 h after treatment (HAT). The R8, R14, and R15 populations translocated only 24.5, 26.5, and 21.9%, respectively. The enzyme activity of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) was not different in the S, R8 and R14 populations. The R15 Population exhibited 165.9 times greater EPSPS activity. Additionally, this population showed a higher EPSPS basal activity and a substitution in the codon 106 from Proline to Serine in the EPSPS protein sequence. EPSPS gene expression in the R15 population was similar to that of S population. In conclusion, the three resistant L. virgata populations show reduced absorption and translocation of 14C-glyphosate. Moreover, a mutation and an enhanced EPSPS basal activity at target-site level confers higher resistance to glyphosate. These results describe for the first time the glyphosate resistance mechanisms developed by resistant L. virgata populations of citrus orchards from Mexico.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

et al. 2016

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“…The resistance mechanism to glyphosate in other countries' goosegrass involves replacement of a proline by serine or threonine at position 106 in the EPSPS gene (P106S or P106T) (Baerson et al, 2002;Ng et al2004;Yu et al2007;Kaundun et al, 2008Kaundun et al, , 2011, or the EPSPS gene amplification in resistant plants (Chen et al, 2015). Such single mutation confers intermediate levels of glyphosate resistance (RF = 2 to 4).…”

mentioning

confidence: 99%

Goosegrass Resistant to Glyphosate in Brazil

et al. 2017

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-Several cases of herbicide resistance in goosegrass have been confirmed worldwide. Reports of control failures after glyphosate application have been observed, especially in the Midwest region of Paraná State. The objective of this study was to evaluate the existence of goosegrass populations resistant to glyphosate. For this, 25 populations collected in two consecutive seasons (2013/ 2014 and 2014/2015) were sown and grown in greenhouse. Glyphosate dose-response curve experiments were performed using doses of 0, 60, 120, 240, 480, 960, 1,920, 3,840, 7,680 and 15,360 g a.e. ha -1 . The application stages were from two to three tillers (E1) for the populations of 2013/2014 and E1 and five to six tillers (E2) for the populations of 2014/2015. Furthermore, three of the populations supposedly considered resistant in these experiments (populations 7, 19 and 25) have had their F1 submitted to the herbicide dose-response test in order to verify whether the resistance was inheritable. With the results obtained in this study, it was concluded that the populations 19 and 25, from Campo Mourão and Luziânia (Midwest of Paraná) are the first confirmed cases of goosegrass resistant to glyphosate in Brazil (RF = 3.99 to 6.81), following all the criteria for confirmation of new weed resistance cases. (2013/2014 e 2014/2015) foram semeadas e cultivadas em casa de vegetação. Experimentos de curva de dose-resposta de glyphosate foram realizados utilizando-se as doses de 0, 60, 120, 240, 480, 960, 1.920, 3.840, 7.680 e 15.360 g e.a. ha -1 Keywords: Eleusine indica, dose-response, application timming, resistance factor. RESUMO -Diversos casos de resistência de capim-pé-de-galinha têm

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Target‐site mutations conferring resistance to glyphosate in feathertop Rhodes grass (Chloris virgata) populations in Australia

Krishnan

Boutsalis

Gill

et al. 2017

Pest Management Science

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Mutations and amplification of EPSPS gene confer resistance to glyphosate in goosegrass (Eleusine indica)

Cited by 93 publications

References 46 publications

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

First Resistance Mechanisms Characterization in Glyphosate-Resistant Leptochloa virgata

Goosegrass Resistant to Glyphosate in Brazil

Target‐site mutations conferring resistance to glyphosate in feathertop Rhodes grass (Chloris virgata) populations in Australia

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