Mutational approach for N2-fixing and P-solubilizing mutant strains of Klebsiella pneumoniae RSN19 by microwave mutagenesis

Li, Jianfeng; Zhang, Shuqing; Shi, She‐Po; Ping-hui, Huo

doi:10.1007/s11274-010-0600-7

Cited by 12 publications

(6 citation statements)

References 32 publications

(32 reference statements)

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“…The maize and soybean roots were shaken carefully inside plastic bags in order to separate the soil from the roots. The rhizosphere soil samples were serially diluted and inoculated on modified Pikovskaya’ s 26 27 agar medium containing 5 g/L insoluble inorganic forms of P (tricalcium phosphate). Petri plates were incubated at 28 °C for 10 days.…”

Section: Methodsmentioning

confidence: 99%

A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

Bai

Dai

et al. 2016

Sci Rep

144

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Phosphate solubilizing fungi (PSF) have huge potentials in enhancing release of phosphorus from fertilizer. Two PSF (NJDL-03 and NJDL-12) were isolated and identified as Penicillium oxalicum and Aspergillus niger respectively in this study. The quantification and identification of organic acids were performed by HPLC. Total concentrations of organic acids secreted by NJDL-03 and NJDL-12 are ~4000 and ~10,000 mg/L with pH values of 3.6 and 2.4 respectively after five-days culture. Oxalic acid dominates acidity in the medium due to its high concentration and high acidity constant. The two fungi were also cultured for five days with the initial pH values of the medium varied from 6.5 to 1.5. The biomass reached the maximum when the initial pH values are 4.5 for NJDL-03 and 2.5 for NJDL-12. The organic acids for NJDL-12 reach the maximum at the initial pH = 5.5. However, the acids by NJDL-03 continue to decrease and proliferation of the fungus terminates at pH = 2.5. The citric acid production increases significantly for NJDL-12 at acidic environment, whereas formic and oxalic acids decrease sharply for both two fungi. This study shows that NJDL-12 has higher ability in acid production and has stronger adaptability to acidic environment than NJDL-03.

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Section: Methodsmentioning

confidence: 99%

A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

Bai

Dai

et al. 2016

Sci Rep

144

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“…Apart from their role as human and animal pathogens, Klebsiella spp. play a role in nitrogen fixation for plants (Govindarajan et al 2007;Li et al 2011). Other importance of these genus includes its bioremediation potential; for instance, Klebsiella variicola has shown potential as eco-friendly biological expedients for the remediation and detoxification of Cr(VI) from the contaminated environments (Kabir et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

et al. 2019

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Coral-associated microbes from Marine National Park (MNP), Gulf of Kutch (GoK), Gujarat, India, were screened for siderophore production. Maximum siderophore-producing isolate NP-C49 and its compound were identified and characterized. The isolate was identified as Klebsiella sp. through 16S rRNA genes sequencing (GenBank accession nos. KY412519 and MTCC 25160). Antibiotic susceptibility profile against 20 commercial antibiotics showed its more sensitivity compared to human pathogenic strain, i.e., Klebsiella pneumonia. The compound was identified as phenazine-1-carboxylic acid (PCA) using the multinuclear ID ( 1 H and 13 C) and 2D ( 1 H-1 H COSY and 1 H-13 C HETCOR) NMR along with high-resolution mass spectrometry. No significant difference in the bacterial growth in the presence of PCA, FeCl 3 and Fe(OH) 3 indicated involvement of factors other than PCA in bacterial growth. The study first reports the identification and characterization of PCA from Klebsiella sp. both from terrestrial and marine sources. Keywords Coral environment • phenazine-1-carboxylic acid • Klebsiella sp. • Gulf of Kutch Communicated by Erko Stackebrandt. Neha P. Patel and M. Raju contributed equally to the manuscript.

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“…The mutagenesis effect of microwave irradiation on T. harzianum was preliminarily studied, the results of existing studies showed that microwave irradiation could cause changes in the activity of antioxidant enzymes in T. harzianum, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO) and so on (Kushwah et al, 2013). We speculated that this not only affected the survival rate of T. harzianum after mutagenesis, but also may be related to its antimicrobial activity and sporulation (Li et al, 2011).…”

Section: Screening Of the T Harzianum Mutant And Genetic Stabilitymentioning

confidence: 99%

Screening of Cucumber Fusarium Wilt Bio-Inhibitor: High Sporulation Trichoderma harzianum Mutant Cultured on Moso Bamboo Medium

Zhang

Xie

et al. 2021

Front. Microbiol.

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Cucumber fusarium wilt is a soil-borne disease which causes serious production decrease in cucumber cultivation world widely. Extensive using of chemical pesticides has caused serious environmental pollution and economic losses, therefore, it is particularly urgent to develop efficient, safe and pollution-free biopesticide. In this study, a mutant strain of Trichoderma harzianum cultivated in moso bamboo medium was proved to be an efficient bio-inhibitor of the disease. The mutant strain T. harzianum T334, was obtained by three microwave mutagenesis cycles with an irradiation power of 600 W and irradiation time of 40 s. In contrast to the original strain, the inhibition rate on cucumber fusarium wilt of the strain T334 increased from 63 to 78%. In this work, disk milling pretreatment of moso bamboo has shown significant beneficial effects on both biotransformation and sporulation of T334. Its sporulation reached 3.7 × 109 cfu/g in mushroom bags with 90% bamboo stem powder (pretreated by disk milli), 9.5% bamboo leaf powder and 0.5% wheat bran when the ratio of solid to liquid was 4:6, the inoculum amount was 10%, and the culture temperature was 28°C. These results provide an alternative bioinhibitor for the control of cucumber fusarium wilt, and a potential usage of moso bamboo in the production of microbial pesticide.

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Mutational approach for N2-fixing and P-solubilizing mutant strains of Klebsiella pneumoniae RSN19 by microwave mutagenesis

Cited by 12 publications

References 32 publications

A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

Characterization of phenazine-1-carboxylic acid by Klebsiella sp. NP-C49 from the coral environment in Gulf of Kutch, India

Screening of Cucumber Fusarium Wilt Bio-Inhibitor: High Sporulation Trichoderma harzianum Mutant Cultured on Moso Bamboo Medium

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