A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

Li, Zhen; Bai, Tongshuo; Dai, Letian; Wang, Fuwei; Tao, Jinjin; Meng, Shiting; Hu, Yunxiao; Wang, Shimei; Hu, Shuijin

doi:10.1038/srep25313

Cited by 131 publications

(92 citation statements)

References 29 publications

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“…2). The major organic acids secreted by this strain were oxalic, tartaric, and citric acids, which was consistent with a previous report by Li et al (2016). However, Tkacz and Lange demonstrated that the main organic acids produced by A. niger are gluconate, citrate, and oxalate (Tkacz and Lange 2004).…”

Section: Discussionsupporting

confidence: 93%

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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Rhizospheric microorganisms can increase phosphorus availability in the soil. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate-solubilizing bacteria. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Among several phosphate-solubilizing fungal isolates screened, strain CS-1 showed the highest phosphorus-solubilization ability. Based on phylogenetic analysis of the internal transcribed spacer region sequence, it was identified as Aspergillus niger. High-performance liquid chromatography analysis revealed that the mechanism of phosphorus solubilization by CS-1 involved the synthesis and secretion of organic acids, mainly oxalic, tartaric, and citric acids. Furthermore, strain CS-1 exhibited other growth-promoting abilities, including efficient potassium release and degradation of crop straw cellulose. These properties help to returning crop residues to the soil, thereby increasing nutrient availability and sustaining organic matter concentration therein. A pot experiment revealed that CS-1 apparently increased the assessed biometric parameters of wheat seedlings, implying the potential of this strain to be developed as a commercial microbial agent. We used Illumina MiSeq sequencing to investigate the microbial community composition in the rhizosphere of uninoculated wheat plants and wheat plants inoculated with the CS-1 strain to obtain insight into the effect of the CS-1 strain inoculation. The data clearly demonstrated that CS-1 significantly reduced the content of pathogenic fungi, including Gibberella, Fusarium, Monographella, Bipolaris, and Volutella, which cause soil-borne diseases in various crops. Strain CS-1 may hence be developed into a microbial agent for plant growth improvement.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0593-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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“…In our study, the pH was decreased in inverse proportion to the increase in soluble P in the two fungi grown in PB medium with hydroxyapatite. Similar results were obtained with different strains of Penicillium and Aspergillus, in which the pH decrease coincided with the increased solubilization of tricalcium phosphate (Pandey et al, 2008, Li et al, 2016. However, in the PB medium containing strengite, the most acidic pH did not coincide with increased levels of solubilization.…”

Section: Discussionsupporting

confidence: 74%

“…Previous studies have indicated that iron phosphate compounds are less susceptible to microbial solubilization than calcium phosphates (Rinu et al, 2013). pH of the medium during growth are efficient phosphate solubilizers (Nahas, 1996;Li et al, 2016). In our study, the pH was decreased in inverse proportion to the increase in soluble P in the two fungi grown in PB medium with hydroxyapatite.…”

Section: Discussionsupporting

confidence: 49%

“…This finding may indicate that the production of organic acids is not the only mechanism for iron phosphate solubilization. Some microorganisms are able to solubilize P from inorganic compounds by simply lowering the pH, which occurs as a result of fungal metabolism and promotes the solubility of some highly soluble phosphate compounds (Barroso and Nahas, 2005;Sharma et al, 2013;Li et al, 2016 (Dorozhkin, 2011;Khan et al, 2014). Because these compounds are hard insoluble P sources, in vitro testing with a culture that includes these compounds is one of the first steps in the selection of microorganisms with a solubilizing capacity (Bashan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The results are shown as the average of the three replicates, in mg L -1 . In considering that efficient phosphate-solubilizing microorganisms tend to decrease the medium pH during their growth (Sharma et al, 2013;Li et al, 2016), we decided to determine the pH of the filtrate for each sample using a potentiometer (Conductronic PC45).…”

Section: Quantitative Estimation Of Phosphate Solubilization In Brothmentioning

confidence: 99%

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Phosphate solubilization by fungi with nematicidal potential

Lima-Rivera

López-Lima

Desgarennes

et al. 2016

J. Soil Sci. Plant Nutr.

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We evaluated the nematicidal potential and phosphate solubilization ability of the fungal species Geomyces pannorum and Paecilomyces carneus, which are associated with the potato cyst nematode Globodera rostochiensis. In a broth medium containing calcium phosphate, the two fungi solubilized between 67%-96% of the insoluble phosphorus that was present in the medium, and in a broth medium containing iron phosphate, the phosphorus that was solubilized by the two fungi ranged between 2%-13%. In a greenhouse experiment, G. pannorum and P. carneus were applied to soil that was naturally infested with G. rostochiensis and planted with Avena sativa. The fungi increased the available phosphorus in the soil by more than 30%, and Paecilomyces carneus also reduced the nematode population by 71%. This study is the first to report on the ability of G. pannorum and P. carneus to increase the available phosphorus in the soil, suggesting that these fungal species may have potential uses in agricultural soils with insoluble phosphorus. Moreover, this study provides a new alternative that contributes to the sustainable management of crops with bio-agents that have dual activity; they increase the available phosphorus in the soil and mitigate plant parasitic nematodes.

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Production of inulin‐ and neolevan‐type fructooligosaccharides by Penicillium janczewskii Zaleski CCIBt 3352

Zaninette

Rocha

Pessoni

et al. 2019

Biotech and App Biochem

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Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of β-fructosyltransferases and/or β-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in a medium supplemented with sucrose concentrations of 30, 100, or 150 g L −1 . Hydrolytic activity on sucrose was observed in culture filtrates; however, at 150 g L −1 sucrose, the accumulation of 8 g L −1 1-kestose (inulin-type FOS) and 7.3 g L −1 neokestose (neolevan-type FOS) was observed, the latter being a type of FOS not commonly produced by filamentous fungi. In addition, minor amounts of four unidentified oligosaccharides, with a high degree of polymerization, were detected. The production of FOS was also observed in enzymatic assays, indicating the presence of extracellular enzymes with transfructosylating activity in the culture filtrates. Our findings demonstrate the feasibility of isolating promising microorganisms, for the production of FOS-synthesizing enzymes, from the rhizosphere of fructan-producing plants of the Brazilian Cerrado. C 2019

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A study of organic acid production in contrasts between two phosphate solubilizing fungi: Penicillium oxalicum and Aspergillus niger

Cited by 131 publications

References 29 publications

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Phosphate solubilization by fungi with nematicidal potential

Production of inulin‐ and neolevan‐type fructooligosaccharides by Penicillium janczewskii Zaleski CCIBt 3352

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