Mutation of <i><scp>RGG</scp>2</i>, which encodes a type B heterotrimeric G protein γ subunit, increases grain size and yield production in rice

Miao, Jun; Yang, Zefeng; Zhang, Dongping; Wang, Yuzhu; Xu, Mingliang; Liang, Guohua; Wang, Jun; Wu, Shujun; Yao, Youli; Du, Xi; Gu, Fangfei; Gong, Zhiyun; Gu, Minghong

doi:10.1111/pbi.13005

Cited by 60 publications

(41 citation statements)

References 65 publications

(90 reference statements)

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“…We concluded that rgb1-1 and rgb1-2 harbor mild alleles of RGB1 as the 3bp and 6-bp deletions did not cause frame shifts. Overexpression of RGG2 in Nipponbare led to a reduced plant height and decreased grain size, whereas the mutants generated via CRISPR/Cas9 in the Zhenshan 97 background exhibited enhanced growth, including elongated internodes, increased 1000-grain weight and plant biomass, and enhanced grain yield per plant [29]. However, the rgg2 CRISPR/Cas9 gene-edited plants under the Sasanishiki genetic background showed a similar phenotype to that of WT (Fig.…”

Section: Discussionmentioning

confidence: 92%

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

Cui

Jiang²,

et al. 2020

BMC Plant Biol

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Background: The heterotrimeric G protein complex, consisting of Gα, Gβ, and Gγ subunits, are conserved signal transduction mechanism in eukaryotes. Recent molecular researches had demonstrated that G protein signaling participates in the regulation of yield related traits. However, the effects of G protein genes on yield components and stress tolerance are not well characterized. Results: In this study, we generated heterotrimeric G protein mutants in rice using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology. The effects of heterotrimeric G proteins on the regulation of yield components and stress tolerance were investigated. The mutants of gs3 and dep1 generated preferable agronomic traits compared to the wild-type, whereas the mutants of rga1 showed an extreme dwarf phenotype, which led to a dramatic decrease in grain production. The mutants showed improved stress tolerance, especially under salinity treatment. We found four putative extra-large G proteins (PXLG)1-4 that also participate in the regulation of yield components and stress tolerance. A yeast two hybrid showed that the RGB1 might interact with PXLG2 but not with PXLG1, PXLG3 or PXLG4. Conclusion: These findings will not only improve our understanding of the repertoire of heterotrimeric G proteins in rice but also contribute to the application of heterotrimeric G proteins in rice breeding.

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Section: Discussionmentioning

confidence: 92%

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

Cui

Jiang²,

et al. 2020

BMC Plant Biol

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“…Overexpression of RGG1 and RGG2 results in small grains (Liu et al ), suggesting that RGG1 and RGG2 restrict grain growth. Recently, it has been shown that loss of function of RGG2 increases grain size (Miao et al ). Currently, it is unclear whether loss of function of RGG1 influences grain size.…”

Section: G Protein Subunits Regulate Grain Size In Ricementioning

confidence: 99%

Control of grain size by G protein signaling in rice

2019

JIPB

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Heterotrimeric G proteins are involved in multiple cellular processes in eukaryotes by sensing and transducing various signals. G protein signaling in plants is quite different from that in animals, and the mechanisms of plant G protein signaling are still largely unknown. Several recent studies have provided new insights into the mechanisms of G protein signaling in rice grain size and yield control. In this review, we summarize recent advances on the function of G proteins in rice grain size control and discuss the potential genetic and molecular mechanisms of plant G protein signaling.

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“…In rice, GS3-GFP and DEP1-GFP were detectable in the membrane and nucleus and function as cofactors of OsMADS1 in regulating grain size (Liu et al, 2018). More recently, the type B Gβ subunit, RGG2, was also found to be localized to plasma membrane, cytoplasm and nucleus (Miao et al, 2019). All of these results suggest that localization of G proteins, including RGG1, to the nucleus is associated with G protein signaling and is involved in transcriptional regulation in rice.…”

Section: Discussionmentioning

confidence: 92%

“…Grain size is one of the important agronomic traits affecting rice yield and quality. Although a complex regulatory gene network related to grain size has been proposed (Miao et al, 2019;Li and Li 2016), the crosstalks between Heterotrimeric G proteins and cytokinin regulatory pathway on grain size controlling are poorly known. In this study, we show that overexpression of RGG1 resulted in decreased plant height and grain length (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…qPE9-1/DEP1 could directly interact with MADS-domain transcriptional factor OsMADS1 and enhance its transcriptional activity to control grain size (Liu et al, 2018). RGG2, encoding a type B Gβ subunit, negatively regulates grain size and also involved in gibberellin signaling (Miao et al, 2019). These prior studies in rice reveal that G proteins play a vital role in determination of grain size as well as in phytohormone regulation.…”

Section: Introductionmentioning

confidence: 99%

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RGG1, involved in the cytokinin regulatory pathway, controls grain size in rice

Tao

Miao

Wang

et al. 2020

Preprint

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Abstract Heterotrimeric GTP binding proteins (G proteins) and cytokinin play important roles in regulating plant growth and development. However, little is known about the mechanism by which they coordinate the regulation of grain size in rice. We functionally characterized one gene, RGG1, encoding a type-A Gγ subunit. Strong GUS staining were detected in young panicles and spikelets, suggesting the role of this gene in modulating panicle-related traits development. By regulating cell division, over-expression of RGG1 in Nipponbare (NIP) and Wuyunjing 30 (WYJ30) significantly decreased plant height, panicle length and grain length. However, rgg1 mutants generated by CRISPR/Cas9 system exhibited no obvious phenotypic differences, which may be due to its extremely low expression level in vivo. Transcriptomic analysis showed that differentially expressed genes (DEGs) were enriched in the cytokinin biosynthesis pathway. We confirmed this result by measuring the endogenous cytokinin levels and showed that the content of cytokinin was lower in OE lines. Additionally, the results of 6-benzylaminopurine (6-BA) treatment showed that increased expression of RGG1 decreased the sensitivity to 6-BA under low concentration. Our results reveal a novel G proteins—cytokinin module controlling grain size in rice, which will be beneficial for understanding the mechanisms of G proteins regulating grain size and plant development.

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Mutation of RGG2, which encodes a type B heterotrimeric G protein γ subunit, increases grain size and yield production in rice

Cited by 60 publications

References 65 publications

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice

Control of grain size by G protein signaling in rice

RGG1, involved in the cytokinin regulatory pathway, controls grain size in rice

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