Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development

Zhang, Shuangxi; Sheng, Huachun; Ma, Yue; Wei, Yanping; Liú, Dan; Dou, Yanhua; Cui, Huiying; Liang, Boyou; Liesche, Johannes; Li, Jisheng; Chen, Shaolin

doi:10.1016/j.jplph.2022.153631

Cited by 2 publications

(1 citation statement)

References 68 publications

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“…Zhang et al 97 showed that mutation of CESA1 phosphorylation site on Thr166 influences pectin synthesis and methylesterification. Although this demonstrates a crosstalk between pectin and cellulose biosynthesis, Thr166 CESA1 phosphorylation is not detectable within the first 15 min after cellotriose application.…”

Section: Resultsmentioning

confidence: 99%

The damage-associated molecular pattern cellotriose alters the phosphorylation pattern of proteins involved in cellulose synthesis and trans -Golgi trafficking in Arabidopsis thaliana

Gandhi

Tseng

Oelmüller

2023

Plant Signaling & Behavior

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We have recently demonstrated that the cellulose breakdown product cellotriose is a damage-associated molecular pattern (DAMP) which induces responses related to the integrity of the cell wall. Activation of downstream responses requires the Arabidopsis malectin domain-containing CELLOOLIGOMER RECEPTOR KINASE1 (CORK1) 1 . The cellotriose/CORK1 pathway induces immune responses, including NADPH oxidase-mediated reactive oxygen species production, mitogen-activated protein kinase 3/6 phosphorylation-dependent defense gene activation, and the biosynthesis of defense hormones. However, apoplastic accumulation of cell wall breakdown products should also activate cell wall repair mechanisms. We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans -Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots. The phosphorylation pattern of enzymes involved in hemicellulose or pectin biosynthesis and the transcript levels for polysaccharide-synthesizing enzymes responded barely to cellotriose treatments. Our data show that the phosphorylation pattern of proteins involved in cellulose biosynthesis and trans -Golgi trafficking is an early target of the cellotriose/CORK1 pathway.

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Section: Resultsmentioning

confidence: 99%

The damage-associated molecular pattern cellotriose alters the phosphorylation pattern of proteins involved in cellulose synthesis and trans -Golgi trafficking in Arabidopsis thaliana

Gandhi

Tseng

Oelmüller

2023

Plant Signaling & Behavior

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Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differential Seed Vigor in Two Contrasting Peanut Genotypes

Zeng

Zhao

et al. 2022

Agriculture

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Seed vigor is an important agronomic trait, and wide variation exists among peanut accessions. However, the detailed regulatory mechanisms underlying differences in seed vigor between varieties are not known in peanut yet. Here, we performed a comparative transcriptome analysis of germinating seeds in two contrasting peanut accessions, namely A86 (high-vigor variety) and A279 (low-vigor variety). A total of 583 and 860 differentially expressed genes (DEGs) were identified at two imbibition stages between A86 and A279, respectively. Pathway enrichment tests highlighted the cell wall remodeling-, hormone signaling-, transcriptional regulation-, and oxidative stress-related DEGs, which may explain to a certain extent the difference in seed vigor between the two cultivars. Among them, the largest number of cell wall remodeling-related DEGs were extensions followed by cellulose synthases, fasciclin-like arabinogalactan proteins, polygalacturonases, expansins, and pectinesterases and the hormone signaling-related DEGs belonged mainly to the auxin and ethylene signaling pathway. The majority of transcriptional regulation-related DEGs were MYB, FAR1, and bHLH transcription factors, and the oxidative stress-related DEGs were mainly peroxidases. Further physiological analyses indicated that differences in seed vigor between A86 and A279 may be associated with differences in the ROS-scavenging abilities mediated by peroxidases. Moreover, we identified 16 DEGs homologous to known Arabidopsis regulators of seed dormancy and germination, suggesting that these DEGs would play similar functional roles during peanut seed germination. Our results not only provide important insights into the difference in seed vigor between varieties, but offer candidate genes that are worth investigating in future studies.

show abstract

Mutation of CESA1 phosphorylation site influences pectin synthesis and methylesterification with a role in seed development

Cited by 2 publications

References 68 publications

The damage-associated molecular pattern cellotriose alters the phosphorylation pattern of proteins involved in cellulose synthesis and trans -Golgi trafficking in Arabidopsis thaliana

The damage-associated molecular pattern cellotriose alters the phosphorylation pattern of proteins involved in cellulose synthesis and trans -Golgi trafficking in Arabidopsis thaliana

Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differential Seed Vigor in Two Contrasting Peanut Genotypes

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