Mutation analysis of ras gene in the liver of European eel (Anguilla anguilla L.) exposed to benzo[a]pyrene

Nogueira, Patrícia; Lourenço, Joana; Mendo, Sónia; Rotchell, Jeanette M.

doi:10.1016/j.marpolbul.2006.05.017

Cited by 39 publications

(15 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nogueira et al (2010), studying Dicentrarchus labrax and Liza aurata in a contaminated coastal lagoon polluted by PAH, observed no differences in the expression levels of ras oncogene among fish from different sites. Similar results were found in Anguilla anguilla exposed to 0.1 and 0.3 μM B[a]P, where the analysis of ras oncogene revealed no differences in levels of expression between control and exposed fish (Nogueira et al , 2006). In another study with mussels ( Mytilus galloprovincialis ) collected in sites with different levels of petrochemical contamination along the NW coast of Portugal, the expression of ras oncogene in digestive gland and gonads was decreased in PAH-contaminated animals.…”

Section: Discussionsupporting

confidence: 84%

“…The oncogene ras is considered one of the most important genes involved in carcinogenesis. The characterization of this gene in several fish species and the presence of ras mutations have already been described in fish populations inhabiting hydrocarbon contaminated areas and following experimental exposure to specific contaminants (Nogueira et al , 2006). In the present study, the ras oncogene was found overexpressed in livers of fish treated with 4, 8, and 16 μmol/kg B[a]P. When comparing these data with DNA damage in erythrocytes, significant differences in DNA damage were apparent only at concentrations of 8 and 16 μmol/kg of B[a]P. Our data suggest that the oncogene ras is expressed even with exposure to low concentrations of the contaminant, and DNA damage is more significant when animals are subjected to higher concentrations.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.

et al. 2017

View full text Add to dashboard Cite

Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α in fish injected with 4 and 8 μmol/kg B[a]P, showing 8.82 and 4.64-fold increases, respectively. Histopathological damage in fish liver was classified as irreparable in fish exposed to 8, 16 and 32 μmol/kg μM B[a]P. The genotoxic damage increased in fish injected with 8 and 16 μmol/kg in comparison with the control group. Acute exposure of B[a]P was capable to interrupt the expression of ras oncogene and hif-1α, and increase DNA breaks due to tissue damage.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 98%

Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Maria et al (2002) described a decrease in blood and liver DNA integrity and an increase in the frequency of erythrocytic nuclear abnormalities, CYP1A protein levels, EROD activity and PAH metabolites in bile from European eel exposed to BaP (Table 2). Nigro et al (2002) and Nogueira et al (2006) observed an elevated DNA damage and a significant induction of apoptosis (cell death) in eel, after exposure to BaP (Table 2). Jha (2004) concluded that BaP induced DNA breaks could lead to induction of chromosomal aberrations which are also associated with initiation and promotion of cancer.…”

Section: The Effects Of Contaminants In European Eel 253mentioning

confidence: 97%

The effects of contaminants in European eel: a review

2009

View full text Add to dashboard Cite

European eel (Anguilla anguilla (L.)) stocks are in decline in most of their geographical distribution and their status is considered below safe biological limits. Recently, there is an increasing awareness that spawner quality might be an essential element in the decline of the species since pollution by bioaccumulating chemical substances may have a large impact on the reproduction success of the eel. This review gives an overview of the literature on the effects of contaminants on the European eel and on the consequences on the biology and fitness of the eel in order to document the role of pollution in its decline. A variety of contaminants have been found to affect the eel. These contaminants may cause disturbance of the immune system, the reproduction system, the nervous system and the endocrine system and effects were reported on several levels of biological organization, from subcellular, organ, individual up to even population level. More extensive research is needed in order to evaluate how pollutants are detrimental to eel populations. Getting a comprehensive overview of the quality (including contamination levels, biomarker responses, lipid content and condition) of the silver eel population all over Europe seems to be an essential and urgent objective for the European eel management.

show abstract

“…Each replicate consisted of one glass beaker containing 150 mL ASTM (plus contaminants in treatment situation). After 24 h exposure, organisms were collected and pooled for the comet assay as described by Nogueira et al (2006). Positive controls were performed by using daphnid cells previously exposed to hydrogen peroxide (H 2 O 2 ).…”

Section: Mixture Testingmentioning

confidence: 99%

Ecotoxicity and genotoxicity of a binary combination of triclosan and carbendazim to Daphnia magna

Silva

Cardoso

Cruz

et al. 2015

Ecotoxicology and Environmental Safety

Self Cite

View full text Add to dashboard Cite

In the environment, chemical substances appear as complex mixtures and consequently organisms are exposed to a variety of chemicals from different sources (e.g. wastewater treatment plants, agriculture runoffs). When studying chemical mixtures, there are two conceptual models usually used to predict toxicity: the Independent Action (IA) and Concentration Addition (CA) models. However, deviations from these reference models can occur as synergism or antagonism, dose ratio or dose level dependency. The aim of the present study was to investigate the effects of triclosan and carbendazim, and their binary mixture to Daphnia magna. With this purpose, immobilisation, feeding inhibition, and reproduction were assessed as main ecotoxicity endpoints. In addition, in vivo genotoxicity of both chemicals was investigated using the comet assay. In the single exposure, carbendazim was more toxic to D. magna than triclosan. When daphnids were exposed to both single compounds, DNA damage was observed. Concerning mixture exposures, different endpoints followed different patterns of response, from additivity: IA model (feeding inhibition and reproduction data), to deviations that indicate interaction between chemicals inside the organism: dose level dependency (immobilisation data) and dose ratio dependency (DNA damage). This study showed that additivity does not rule the dose-effect relation in chemical mixtures of carbendazim and triclosan and interactions between both chemicals might induce generally higher toxicity than predicted based on single chemical exposures.

show abstract

Mutation analysis of ras gene in the liver of European eel (Anguilla anguilla L.) exposed to benzo[a]pyrene

Cited by 39 publications

References 23 publications

Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.

Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.

The effects of contaminants in European eel: a review

Ecotoxicity and genotoxicity of a binary combination of triclosan and carbendazim to Daphnia magna

Contact Info

Product

Resources

About