Mutagenicity in Surface Waters: Synergistic Effects of Carboline Alkaloids and Aromatic Amines

Muz, Melis; Krauß, Martin; Kutsarova, Stela; Schulze, Tobias; Brack, Werner

doi:10.1021/acs.est.6b05468

Cited by 59 publications

(51 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nontarget screening has been shown in many EDA studies (Muz et al 2017a(Muz et al , 2017bMuschket et al 2018) to be the method of choice to detect and identify candidates in active fractions. In the case of compounds that are potent at ultratrace concentrations such as steroidal hormones, this approach runs the risk of failing due to insufficient LODs of NTS.…”

Section: Confirmationmentioning

confidence: 99%

Effect‐Directed Analysis of Progestogens and Glucocorticoids at Trace Concentrations in River Water

Hashmi

Krauß

Escher

et al. 2019

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Effect-based monitoring is increasingly applied to detect and-in conjunction with chemical analysis-to identify endocrine-disrupting compounds (EDCs) in the environment. Although this approach of effect-directed analysis has been successfully demonstrated for estrogenicity and androgenicity, data on progestogens and glucocorticoids driving endocrine disruption are quite limited. We investigated progestogenic and glucocorticoid activities in Danube River water receiving untreated wastewater from Novi Sad, Serbia. After a 2-step fractionation, all fractions were tested with reporter gene bioassays for agonistic and antagonistic hormonal responses at progestogenic and glucocorticoid hormone receptors as well as with target and nontarget analytical screening of active fractions by liquid chromatography-high-resolution mass spectrometry. Due to masking by cytotoxic mixture components, the effects could not be detected in the raw water extract but were unraveled only after fractionation. Target chemical screening of the fraction that was active in the progesterone receptor (PR) assay revealed that progesterone and megestrol acetate were predominant drivers of PR-mediated activity along with medroxyprogesterone, dihydrotestosterone, androsterone, and epiandrosterone. Hydrocortisone was detected at sub-ng/L concentration in the active fraction in the glucocorticoid receptor (GR) assay but could not explain a significant fraction of the observed GR activity. The present study indicates that effect-based monitoring is a powerful tool to detect EDCs in the aquatic environment but that fractionation may be required to avoid masking effects of mixture components. Future effect-directed analysis studies are required to better understand the occurrence of EDCs and masking compounds in different lipophilicity windows, to finally reduce fractionation requirements for monitoring to a smart clean-up. Environ

show abstract

Section: Confirmationmentioning

confidence: 99%

Effect‐Directed Analysis of Progestogens and Glucocorticoids at Trace Concentrations in River Water

Hashmi

Krauß

Escher

et al. 2019

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Site-specific compounds from local economic and social activities, such as in this example, typically go Effect-based monitoring combined with EDA and mixture effect experiments also support the identification and understanding of effects driven by the interaction of different compounds. This has been demonstrated by partial unraveling of mutagenic effects in the river Rhine, establishing synergistic effects of industrial aromatic amines with natural carboline alkaloids, which co-occur frequently in river water [46].…”

Section: Effect-directed Analysismentioning

confidence: 99%

Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures

et al. 2019

Self Cite

View full text Add to dashboard Cite

Environmental water quality monitoring aims to provide the data required for safeguarding the environment against adverse biological effects from multiple chemical contamination arising from anthropogenic diffuse emissions and point sources. Here, we integrate the experience of the international EU-funded project SOLUTIONS to shift the focus of water monitoring from a few legacy chemicals to complex chemical mixtures, and to identify relevant drivers of toxic effects. Monitoring serves a range of purposes, from control of chemical and ecological status compliance to safeguarding specific water uses, such as drinking water abstraction. Various water sampling techniques, chemical target, suspect and non-target analyses as well as an array of in vitro, in vivo and in situ bioanalytical methods were advanced to improve monitoring of water contamination. Major improvements for broader applicability include tailored sampling techniques, screening and identification techniques for a broader and more diverse set of chemicals, higher detection sensitivity, standardized protocols for chemical, toxicological, and ecological assessments combined with systematic evidence evaluation techniques. No single method or combination of methods is able to meet all divergent monitoring purposes. Current monitoring approaches tend to emphasize either targeted exposure or effect detection. Here, we argue that, irrespective of the specific purpose, assessment of monitoring results would benefit substantially from obtaining and linking information on the occurrence of both chemicals and potentially adverse biological effects. In this paper, we specify the information required to: (1) identify relevant contaminants, (2) assess the impact of contamination in aquatic ecosystems, or (3) quantify cause-effect relationships between contaminants and adverse effects. Specific strategies to link chemical and bioanalytical information are outlined for each of these distinct goals. These strategies have been developed and explored using case studies in the Danube and Rhine river basins as well as for rivers of the Iberian Peninsula. Current water quality assessment suffers from biases resulting from differences in approaches and associated uncertainty analyses. While exposure approaches tend to ignore data gaps (i.e., missing contaminants), effect-based approaches penalize data gaps with increased uncertainty factors. This integrated work suggests systematic ways to deal with mixture exposures and combined effects in a more balanced way, and thus provides guidance for future tailored environmental monitoring. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

show abstract

“…The latter is produced by bracken fern and emitted into the water cycle particularly during rain events at toxicologically relevant concentrations (Clauson-Kaas et al 2016;Ojika et al 1987). Recently, natural carboline alkaloids have been demonstrated to exhibit synergistic mutagenic effects with anthropogenic aromatic amines (Muz et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Target screening of plant secondary metabolites in river waters by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS)

Nanusha

Krauß

Schönsee

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Substantial efforts have been made to monitor potentially hazardous anthropogenic contaminants in surface waters while for plant secondary metabolites (PSMs) almost no data on occurrence in the water cycle are available. These metabolites enter river waters through various pathways such as leaching, surface run-off and rain sewers or input of litter from vegetation and might add to the biological activity of the chemical mixture. To reduce this data gap we conducted a LC-HRMS target screening in river waters from two different catchments for 150 plant metabolites which were selected from a larger database considering their expected abundance in the vegetation, their potential mobility, persistence and toxicity in the water cycle and commercial availability of standards. Results: The screening revealed the presence of 12 out of 150 possibly toxic PSMs including coumarins (bergapten, scopoletin, fraxidin, esculetin and psoralen), a flavonoid (formononetin) and alkaloids (lycorine and narciclasine). The compounds narciclasine and lycorine were detected at concentrations up to 3 µg/L while esculetin and fraxidin occurred at concentrations above 1 µg/L. Nine compounds occurred at concentrations above 0.1 µg/L, the Threshold for Toxicological Concern (TTC) for non-genotoxic and non-endocrine disrupting chemicals in drinking water. Conclusions: Our study provides an overview of potentially biologically active PSMs in surface waters and recommends their consideration in monitoring and risk assessment of water resources. This is currently hampered by a lack of effect data including toxicity to aquatic organisms, endocrine disruption and genotoxicity and demands for involvement of these compounds in biotesting.

show abstract

Mutagenicity in Surface Waters: Synergistic Effects of Carboline Alkaloids and Aromatic Amines

Cited by 59 publications

References 80 publications

Effect‐Directed Analysis of Progestogens and Glucocorticoids at Trace Concentrations in River Water

Effect‐Directed Analysis of Progestogens and Glucocorticoids at Trace Concentrations in River Water

Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures

Target screening of plant secondary metabolites in river waters by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS)

Contact Info

Product

Resources

About