Mutagenic synergy between paraoxon and plant-activatedm-phenylenediamine or 2-acetoxyacetylaminofluorene

Gichner, T.; Velemínský, J.; Wagner, Elizabeth D.; Plewa, Michael J.

doi:10.1002/(sici)1098-2280(1996)27:1<59::aid-em8>3.0.co;2-9

Cited by 7 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interactions with neutral DMPC vesicles which mimics the external leaflet of the plasma membrane demonstrates internalization to the targeted cell, and interaction with anionic DMPG vesicles represents escaping from endosome. To investigate the interaction between model membrane vesicles DMPC, DMPG, and Polymer/DNA complexes, DNA was labeled with EtBr, which is a DNA intercalation agent and represents an increase in the fluorescence intensity when bounded to DNA . Electrostatic interaction is mainly favored with phosphate groups in double stranded DNA.…”

Section: Resultsmentioning

confidence: 99%

Biophysical characterization of quaternary pyridinium functionalized polynorbornenes for DNA complexation and their cellular interactions

et al. 2017

View full text Add to dashboard Cite

Cationic polymers with hydrophobic side chains have gained great interest as DNA carriers since they form a compact complex with negatively charged DNA phosphate groups and interact with the cell membrane. Amphiphilic polyoxanorbornenes with different quaternary alkyl pyridinium side chains with ethyl-p(OPy2) and hexyl units-p(OPy6) bearing 10 kDa MWT were synthesized by living Ring-Opening Metathesis Polymerization method. The physicochemical characteristics: critical micellar concentration, size distribution, surface charge, and condensation of polymer/DNA complex were investigated. Morphology of complexes was monitored by Atomic force microscopy. Cytotoxicity and interaction of these complexes with model lipid vesicles mimicking the cell membrane were examined. These polymers were enabled to form small sized complexes of DNA, which interact with model membrane vesicles. It was found that the nature of hydrophobicity of the homopolymers significantly impacts rates of DNA complexation and the surface charge of the resulting complexes. These results highlight the prospect of the further examinations of these polymers as gene carriers.

show abstract

Section: Resultsmentioning

confidence: 99%