“…Similar results were reported with other species of Poaceae family (Busey, 1980;Krishna et al, 1984;Hase et al, 1999;Zaka et al, 2002). Results showed that gamma irradiation doses of 70 to 113 Gy were successful in creating bermudagrass mutants, concurring with earlier reports (Powel et al, 1974;Burton, 1985;Hanna et al, 1997b;Caetano-Anolles, 1999;Hanna and Elsner, 1999;Lu et al, 2009 Lu et al, (2009) and (Li et al, 2010), respectively.…”
Bermudagrass, Cynodon dactylon (L.) Pers. is a widely used warm-season turfgrass species in warmer regions of the world. Gamma (ɣ) irradiation has been used to generate useful variations in turfgrass breeding for various morphological traits. The objective of the present study was to measure and determine variations in morphology and turfgrass characteristics of a native drought resistant bermudagrass germplasm irradiated with 70, 90 or 110 Gy using a 60 Co source. The stolons containing a single node were irradiated and immediately planted for regeneration in a greenhouse at the Akdeniz University, Antalya, Turkey. Selected mutants regenerated from the irradiated stolons were clonally propagated and transplanted into plastic pots for further observations of turfgrass characteristics. Survival rates of stolons exposed to 70, 90 and 110 Gy were 76%, 43% and 17% respectively, 6 weeks after treatment. Dosages of 85 and 57 Gy were determined as LD50 and LD20 for the cuttings, respectively. The linear reduction of survival rate with increasing gamma-rays was highly correlated (r 2 =0.99). A total of four mutant lines (0.3 % of the irradiated plants) showed a distinct dwarfed growth habit. Three of these lines were originated from 70 Gy and one from 110 Gy. These mutant lines exhibited more dwarf growth habit, higher shoot density, finer leaf texture than parental genotype. Mutant lines developed in this study can be used for the development of improved bermudagrass cultivars for landscaping and sports turf.
“…Similar results were reported with other species of Poaceae family (Busey, 1980;Krishna et al, 1984;Hase et al, 1999;Zaka et al, 2002). Results showed that gamma irradiation doses of 70 to 113 Gy were successful in creating bermudagrass mutants, concurring with earlier reports (Powel et al, 1974;Burton, 1985;Hanna et al, 1997b;Caetano-Anolles, 1999;Hanna and Elsner, 1999;Lu et al, 2009 Lu et al, (2009) and (Li et al, 2010), respectively.…”
Bermudagrass, Cynodon dactylon (L.) Pers. is a widely used warm-season turfgrass species in warmer regions of the world. Gamma (ɣ) irradiation has been used to generate useful variations in turfgrass breeding for various morphological traits. The objective of the present study was to measure and determine variations in morphology and turfgrass characteristics of a native drought resistant bermudagrass germplasm irradiated with 70, 90 or 110 Gy using a 60 Co source. The stolons containing a single node were irradiated and immediately planted for regeneration in a greenhouse at the Akdeniz University, Antalya, Turkey. Selected mutants regenerated from the irradiated stolons were clonally propagated and transplanted into plastic pots for further observations of turfgrass characteristics. Survival rates of stolons exposed to 70, 90 and 110 Gy were 76%, 43% and 17% respectively, 6 weeks after treatment. Dosages of 85 and 57 Gy were determined as LD50 and LD20 for the cuttings, respectively. The linear reduction of survival rate with increasing gamma-rays was highly correlated (r 2 =0.99). A total of four mutant lines (0.3 % of the irradiated plants) showed a distinct dwarfed growth habit. Three of these lines were originated from 70 Gy and one from 110 Gy. These mutant lines exhibited more dwarf growth habit, higher shoot density, finer leaf texture than parental genotype. Mutant lines developed in this study can be used for the development of improved bermudagrass cultivars for landscaping and sports turf.
“…Sparrow et al (1958) reported that reduced stem elongation is due to reduced nutrition contents, and the mechanism of assimilation may also be an important factor. Similar results have been observed by many authors, for example by Cheng and Gao (1988) in barley and by Krishna et al (1984) in Chloris gayana. Remarkable height inhibition has been observed by chemical mutagens in several plants , Reddy 1992, Nabipour et al 2004, Stamo et al 2007).…”
SummaryThe present study evaluates the genotoxic and cytotoxic effects of the crude water and ethanol extracts of Peganum harmala L. (Zygophyllaceae) on the Vicia faba L. M1 generation. Two types of treatments were carried out in this study. In the first type, the germinated root tips were treated with four dilutions of the water extract and ethanol extract (12.5, 25, 50 and 100%). All concentrations were applied for 3, 6, 12 and 24 h. In the second treatment, soaked V. faba seeds were treated with the same concentrations of the two extracts for 3, 6, 12, and 24 h, and then planted to raise the M1 generation. Root tips were squashed after Feulgen staining, and the prepared slides were analyzed for chromosomal aberrations and mitotic index changes. Significant concentrationdependent increase and duration-dependent decrease of MI were observed with aqueous extracts, while with ethanol extracts, the MI showed concentration-and duration-dependent decrease. The two extracts induced significant (p 0.01) increase in the percentage of nuclear and chromosomal aberrations. Concentration-and duration-dependent increase in the number of branches per plant and decrease in the other studied growth parameters were also recorded for all treatments with the water and ethanol extracts.
“…Chlorina: light green colour of the leaves; some died within 20 days, however, few vigorous survives and flowered and withered without fruits Xantha: bright yellow colour of the leaves and Albino: white leaf with no pigmentation. Similar abnormalities have also been reported in Chloris guana (Krishna et al,.1984), in Vicia faba (Vandana and Dubey, 1988;Vandana, 1992;Kumar et al, 1993;Bhat et al, 2007), in Vigna radiata L. (Khan and Siddiqui, 1993) by MMS and gamma ray treatments. The leaf abnormalities may also be due to actual mutation processes which are most easily induced in leguminous plants (Blixt, 1972) or due to chromosomal alterations (Grover and Virk, 1984).…”
Section: Results and Disscussionmentioning
confidence: 51%
“…Griffith and Johnson (1962) and Srivastava (1979) reported that reduction in germination percentage was due to weakening and disturbances of growth process, regulated in early elimination of seedlings. Krishna et al (1984) considered that the inhibition of germination may be due to interaction between mutagen and the seed cell system. It may also be due to toxicity of mutagens followed by mutational changes at genic or chromosomal level because the reduction in germination corresponds with the increasing chromosomal aberrations (Laskar and Khan, 2014).…”
In order to broaden the genetic base of lentil, induced chemical mutagenesis was performed. Dry and healthy seeds of lentil cultivars Pant L 406 and DPL 62 were treated with 0.02% MMS (6hrs), 0.04% MMS (6hrs), 0.02% MMS (9hrs) and 0.04% MMS (9hrs) prepared in sodium phosphate buffer at 7.0 pH for 6 hours at room temperature. Bio-physiological observations showed almost a direct relationship with the MMS concentrations and treatment duration, where inhibition and sterility increases with increase in concentration and duration. The 0.02% and 0.04% for 6 hrs treatments of MMS were found effective in inducing sufficient genetic variability in both the cultivars of lentil especially for selection of yield attributing traits, and 0.04% for 9 hrs induced higher desirable phenotypic variations with few unique phenotypes of future interest, whereas 0.04% for 9 hrs found to have highest lethal dose value and % variations in the all variant small survival population. Plant habit and leaf morphology were found most sensitive phenotypic category towards the MMS doses used. Also the character association between yield and other agronomic traits were altered and improved due to the mutagenic treatments. Overall, the results were promising and useful for future crop improvement work on lentil.
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