Mutagenic evaluation of trichlorfon using different assay methods with <i>Salmonella typhimurium</i>

Barrueco, Carmen; Herrera, Antonio; Pe≁a, Eduardo de la

doi:10.1093/mutage/6.1.71

Cited by 9 publications

(2 citation statements)

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“…Viability Assays. To quantify the bacterial toxicity of the test compounds we performed a viability assay in which we determined the number of viable bacteria per milliliter after treatment. , The overnight cultures were the same as those used for the mutagenicity assay; 100 μL of the test chemical solution or DMSO, 100 μL of an appropriate dilution from the overnight culture of the tester strain, and 500 μL of buffer phosphate or S9 mix (containing 10% S9) were added to 2 mL of top agar supplemented with 0.2 mL of a 25 mM histidine/0.5 mM biotin solution and shaken. The mixture was spread onto a fresh nutrient agar plate.…”

Section: Methodsmentioning

confidence: 99%

Insecticidal and Mutagenic Evaluation of Two Annonaceous Acetogenins

et al. 2000

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Annonaceous acetogenins represent a new class of bioactive compounds whose primary mode of action is the inhibition of NADH-ubiquinone oxidoreductase. Given the potential pesticidal use of such a class of compounds, we have further evaluated the antifeedant and insecticidal effects of squamocin and annonacin, two annonaceous acetogenins, on Spodoptera littoralis, Leptinotarsa decemlineata, and Myzus persicae. Additionally, to partially assess their environmental risk, we have also tested their mutagenicity in Salmonella typhimurium strains TA98, TA100, and TA102 in the presence and absence of a metabolic activation system. Among the test compounds, annonacin showed antifeedant effects on L. decemlineata, while squamocin was toxic to L. decemlineata and M. persicae. Neither acetogenin was mutagenic, although both were toxic in the absence of a metabolic activation system. We compared these results with those obtained with rotenone, a well-known respiratory inhibitor that was highly toxic to L. decemlineata and M. persicae and showed no mutagenicity/toxicity in the S. typhimurium strains tested up to a concentration of 1000 microg per plate.

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Section: Methodsmentioning

confidence: 99%

Insecticidal and Mutagenic Evaluation of Two Annonaceous Acetogenins

et al. 2000

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“…References: 1(Ames et al, 1975); 2 (Maron and Ames, 1983); 3(Barrueco et al, 1991); 4(Kado et al, 1986); 5(Ulitzur et al, 1980);6 …”

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Bioanalytical Tools in Water Quality Assessment

Escher¹

2011

wio

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The publisher makes no representation, express or implied, with regard to the accuracy of the information contained in this book and cannot accept any legal responsibility or liability for errors or omissions that may be made. DisclaimerThe information provided and the opinions given in this publication are not necessarily those of IWA and should not be acted upon without independent consideration and professional advice. IWA and the Editors and Authors will not accept responsibility for any loss or damage suffered by any person acting or refraining from acting upon any material contained in this publication.

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Organophosphorus Compounds

Storm¹

2001

Patty's Toxicology

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Organophosphate pesticides are a highly diverse group of chemicals to which workers may be exposed during manufacture and formulation and during or after application for their intended uses. They are all characterized by their ability to inhibit the enzyme acetylcholinesterase (AChE) that deactivates the neurotransmitter acetylcholine (ACh). Compounds in this class are numerous and have been categorized in many ways according to the nature of the substituents. Gallo and Lawryk, for example, categorized them into four main categories (Groups I‐IV) based on the characteristics of the leaving group (X). Group I compounds, phosphorylcholines, have a leaving group that contains a quaternary nitrogen and are among the most potent organophosphates (e.g., Shradan). Group II compounds, fluorophosphates, have a fluoride leaving group and are also generally highly toxic (e.g., diisopropyl fluorophosphate). Group III compounds have leaving groups that contain cyanide or a halogen other than fluoride and are generally less potent than Groups I or II (e.g., Parathion). Group IV contains most of the organophosphates used as insecticides today. These compounds have alkoxy, alkylthio, aryloxy, arylthio or heterocyclic leaving groups and a wide variety of other substituents. Another classification scheme is based on the nature of the atoms that immediately surround the central phosphorus atom and results in 14 different categories. According to this scheme, phosphates are the prototype for the entire class and are those compounds where all four atoms that surround the phosphorus atom are oxygen (e.g., dichlorvos, mevinphos). Sulfur‐containing organophosphate compounds (phosphorothioates; phosphorothiolates; phosphorodithioates; phosphorodithiolates) are far more numerous than phosphates and include well recognized organophosphate insecticides such as parathion, diazinon, chlorpyrifos, etc. Other groups contain nitrogen (phosphoramides and phosphorodiamides), nitrogen and sulfur (phosphoramidothionates and phosphoramidothiolates), carbon (phosphonates and phosphinates), or carbon and sulfur (phosphonothionates, phosphonothionothiolates and phosphinothionates). All aspects of organophosphate chemistry, toxicity, analysis, and exposure potential have been previously reviewed. Additionally information regarding the toxicity of this class of compounds has expanded greatly in recent years as a result of toxicity data supplied by registrants to the U.S. EPA's Office of Pesticides to support reregistration. These data are being made publically available by the U.S. EPA on their internet web site. The following discussion draws heavily from recent reviews but also includes summaries of relevant toxicity data submitted to the U.S. EPA available when this chapter was completed. Due to space limitations detailed data reviews are included here for only 30 organophosphate pesticides. Information on other pesticides registered or undergoing reregistration in the United States can be readily obtained from the previously mentioned website.

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Mutagenic evaluation of trichlorfon using different assay methods with Salmonella typhimurium

Abstract: Evaluation of the mutagenicity of trichlorfon pesticide was carried out with strains TA1535, TA100, TA97, TA98 and

Cited by 9 publications

References 9 publications

Insecticidal and Mutagenic Evaluation of Two Annonaceous Acetogenins

Insecticidal and Mutagenic Evaluation of Two Annonaceous Acetogenins

Bioanalytical Tools in Water Quality Assessment

Organophosphorus Compounds

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