Mutagenic effects of heavy ion radiation in plants

Mei, Mantong; Deng, Hui; Lu, Yang-Jiang; Zhuang, C.; Liu, Z.; Qiu, Qi; Qiu, Yongjian; Yang, T. C.

doi:10.1016/0273-1177(94)90489-8

Cited by 74 publications

(38 citation statements)

References 15 publications

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“…Therefore, the mutation frequencies per unit dose were higher with ion beams than with gamma rays, thus indicating that ion beams had a higher mutation effectiveness than gamma rays, which confirms the results of previous studies (Fujii et al 1966, Mei et al 1994.…”

Section: Discussionsupporting

confidence: 88%

“…Konzak et al (1965) suggested that the usefulness of any mutagen in plant breeding depends not only on its mutagenic effectiveness, but also on its mutagenic efficiency. In this sense, ion beams have been shown to exhibit higher effectiveness compared to gamma rays (Fujii et al 1966, Mei et al 1994, X-rays (Hirono et al 1970, Yang andTobias 1979), and electrons . However, the efficiency of ion beams has not been compared to that of gamma rays or X-rays.…”

Section: Introductionmentioning

confidence: 99%

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Mutagenic effects of ion beam irradiation on rice

et al. 2009

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We investigated the usefulness of ion beams for mutation breeding in rice (Oryza sativa L.) by comparing the efficiency (i.e., the ratio of desirable mutations to plant damage such as lethality and sterility), mutation rate, spectrum, and optimum dose to that of gamma rays. Rice seeds were irradiated with carbon ions (mean linear energy transfer = 76 and 107 keV/µm), helium ions (9 keV/µm), and gamma rays, and their survival and fertility were examined in the M 1 generation. The frequency of chlorophyll mutations and their types (albina, xantha, and viridis) were examined in the M 2 generation, using the M 1 -plant progeny method. The efficiency of ion beams either equaled or exceeded that of gamma rays. In addition, the mutation rate of ion beams was higher than that of gamma rays. Thus, ion beams appeared to efficiently induce mutants with little radiation damage. No remarkable difference was observed in the relative frequencies of each type of mutation among 3 types of ion beams and gamma rays, thus suggesting that there was no difference in the spectrum. A shoulder dose, which hardly affected survival, was sufficient to efficiently obtain mutants for both types of radiation.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Mutagenic effects of ion beam irradiation on rice

et al. 2009

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“…Few investigations, however, have been performed on the molecular nature of the structural alterations of the DNA induced by ion particles. Only Mei et al (1994) found that a rearrangement had occurred in the genome of dwarf mutant of rice induced by argon ions, using random genomic clones as probes.…”

mentioning

confidence: 99%

Molecular analysis of carbon ion-induced mutations in Arabidopsis thaliana.

et al. 1998

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In order to elucidate the characteristics of the mutations induced by ion particles at the molecular level in plants, mutated loci in carbon ion-induced mutants of Arabidopsis were investigated by PCR and Southern blot analyses. In the present study, two lines of gl1 mutant and two lines of tt4 mutant were isolated after carbon ion-irradiation. Out of four mutants, one had a deletion, other two contained rearrangements, and one had a point-like mutation. From the present result, it was suggested that ion particles induced different kinds of alterations of the DNA and therefore they could produce various types of mutant alleles in plants.

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“…The frequencies of mitotic cells with chromosome aberrations were significantly enhanced by ion beam exposure, although the doseeffect was not recognized (Table 2). Mei et al (1994) demonstrated that high-LET ions induced chromosome deletion and rearrangement. In the present experiment, chromosome fragments were frequent at all doses of C and He ion beams.…”

Section: Introductionmentioning

confidence: 99%

Potato Virus Y-resistant Mutation Induced by the Combination Treatment of Ion beam Exposure and Anther Culture in Nicotiana tabacum L.

Hamada

Inoue

Tanaka

et al. 1999

Plant Biotechnology

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To develop an efficient procedure for obtaining a desired mutation, we performed ion beam exposure on tobacco anthers and selected Potato Virus Y (PVY)-resistant mutants in the haploid generation.Anthers were exposed to C ion beams of 5 to 200Gy or He ion beams of 5 to 400Gy, and cultured on modified Nakata's medium. Anther culture response, the percentage of anthers producing plantlets, was markedly reduced by the exposure and, at higher doses, all plantlets were vitrified. RD50 based on the anther culture response was about 5Gy for C ion beams and about 10Gy for He ion beams. The frequency of mitotic cells with chromosome aberrations in root tips of plantlets derived from exposed anthers, ranged from 11.9% to 16.4%, compared to 3.1% in the control.All plants in the non-exposed regimen died within 21 days after inoculation of PVY. On the other hand, 15 of 472 plants in the exposed regimen were viable and continued to grow, with minor disease symptoms observed in some plants after 50 days. Accordingly, these plants were considered to be PVYresistant. In particular, one plant showed no disease symptoms and grew vigorously.

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Mutagenic effects of heavy ion radiation in plants

Cited by 74 publications

References 15 publications

Mutagenic effects of ion beam irradiation on rice

Mutagenic effects of ion beam irradiation on rice

Molecular analysis of carbon ion-induced mutations in Arabidopsis thaliana.

Potato Virus Y-resistant Mutation Induced by the Combination Treatment of Ion beam Exposure and Anther Culture in Nicotiana tabacum L.

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