Mutagenic Effect on Seed Germination, Seedling Growth and Seedling Survival of Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp)

Abstract

In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30 th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD 50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD 50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.

Effect of gamma irradiations on seed germination, seedling growth and mutation induction in Cluster bean [Cyamopsis tetragonoloba (L.) Taub.]

“…Gamma irradiated dicotyledonous seeds depict distinctive high germination and survival for long time probably with the support of cotyledonary leaves (Dubinin, 1964) providing a chance to repair to certain extent (Gaul 1958). Contrary to our observations an inverse relationship between survival and dose of mutagen have been reported in different leguminous crops with a gradual change in chickpea (Umavathi and Mullanainathan 2015), pigeon pea (Ariraman et al 2014), cowpea (Horn et al 2016), greengram (Vairam et al 2014) andblackgram (Markeen et al 2007). The drastic effect on seedling survival between 700 to 800 Gy places LD 50 in a very narrow range of radiation treatment (Fig.…”

Influence of Gamma Irradiation and Seed Treatment Chemicals on Seed Longevity of Bengal Gram (Cicer arietinum L.) and Black Gram (Vigna mungo L.)

“…Likewise, Narayan et al, (2014) noticed decrease in the seed germination with an increase in gamma irradiation dosage from 200 to 800 Gy and they also reported that the regeneration potential of callus derived from primary leaves of treated seeds improved at low dosage (200 to 400 Gy). In the same line, Ariramana et al, (2014) in pigeon pea, Monica and Seetharaman (2014) in Lablab purpureus and Dhulgande et al, (2015) in pea reported inhibition of seed germination and seedling growth at higher doses or concentrations of mutagens. While, Muhammad Amjad and Muhammad Akbar (2002) reported higher electrical conductivity of onion seeds exposed to gamma irradiation (10, 20, 40, 80 and 100 Krad) than that of un irradiated seeds.…”

Gamma Irradiation - A Tool for Enhancing Storage Life of Grain Cowpea (Vigna unguiculata (L.) Walp.)

“…Sometimes the reduction in physiological traits may be due to sudden destruction of growth inhibitors and metabolic changes [21]. No morphological abnormalities was observed in control and lower doses of gamma rays , whereas few crinkled leaves were found in higher doses like (T 4 ) 400 Gy and (T 5 ) 500 Gy in the early days.…”