Mutagen Formation in the Reaction of Nitrite with the Food Components Analogous to Sorbic Acid

Flavouring Group Evaluation 62 Rev1 (FGE.62 Rev1): Consideration of of linear and branched-chain aliphatic unsaturated, unconjugated alcohols, aldehydes, acids, and related esters evaluated by JECFA (61st and 68th meeting) structurally related to branched

“…The study is considered valid. Rec assay B. subtilis 100 -1000 μg/plate Negative 2 (Osawa & Namiki, 1982) The validity of this study is unclear. SCE test CH V79 2.5 -10 μg/ml Negative (Kinsella, 1982) Not cytotoxic.…”

“…The maximum doses reported for these studies ranged from 333 to 50000 microgram/plate. In further bacterial assays, such as the recassay utilizing Bacillus subtilis, incubated with oleic acid (Osawa & Namiki, 1982), the His + reversion assay utilizing Salmonella typhimurium incubated with methyl linoleate [FL-no: 09.646] or methyl linolenate (MacGregor et al, 1985), and a modified Ames test utilizing Escherichia coli WP2uvrA incubated with oleic acid (Shimizu et al, 1985), these aliphatic unsaturated non-conjugated acids and esters were non-mutagenic.…”

Scientific Opinion on Flavouring Group Evaluation 95 (FGE.95): Consideration of aliphatic, linear or branched-chain saturated and unsaturated alcohols, aldehydes, acids and related esters evaluated by JECFA (69th meeting) structurally related to esters of

“…Rec assay B. subtilis 1.0 mg/plate Negative 1 (Osawa & Namiki, 1982) Sister Chromatid Exchange CH V79 2.5 -10 µg/ml Negative (Kinsella, 1982) Chromosome aberrations CH V79 2.5 -10 µg/ml Positive (Kinsella, 1982) No data on cytotoxicity reported.…”

Flavouring Group Evaluation 5, Revision 2 (FGE.05Rev2): Branched- and straight-chain unsaturated carboxylic acids and esters of these with aliphatic saturated alcohols from chemical groups 1, 2, 3 and 5

“…The maximum doses reported for these studies ranged from 333 to 50000 microgram/plate. In further bacterial assays, such as the rec-assay utilizing Bacillus subtilis, incubated with oleic acid (Osawa & Namiki, 1982), the His + reversion assay utilizing Salmonella typhimurium incubated with methyl linoleate or methyl linolenate [FL-no: 09.646] (MacGregor et al, 1985), and a modified Ames test utilizing Escherichia coli WP2uvrA incubated with oleic acid (Shimizu et al, 1985), these aliphatic unsaturated nonconjugated acids and esters were non-mutagenic.…”