Mussel-Inspired Peptide Coatings on Titanium Implant to Improve Osseointegration in Osteoporotic Condition

Abstract

Osteoporosis greatly impairs in vivo implant osseointegration because of poor osteogenesis in osteoporotic conditions and the low bioactivity of implants, such as titanium-based biomaterials. Various surface engineering strategies, including unstable physical absorption or complex chemical conjugations, have been developed to biofunctionalize titanium implants and improve interfacial osseointegration. However, very few of them took into consideration the clinically challenging osteoporotic condition, as well as dual-functionalization of the implants for improvement of both osteoblast adhesion and osteogenesis. In this work, we combined two mussel-inspired bioactive peptides (i.e., with cell adhesive or osteogenic sequences) for one-step dual-functionalization of Ti screws via a facile self-organized multivalent coordinative interaction. In vitro study indicated that the biomimetic dual-functional coating could efficiently improve the osteogenesis of osteoporosis-derived mesenchymal stem cells despite of their impaired bone metabolism. Moreover, under osteoporotic in vivo condition, the dual-functional peptide coating on Ti screws could also give rise to significant enhancement of interfacial osteogenesis, newly formed bone condition, osseointegration, as well as implant mechanical stability. This is probably due to the integrin-targeted cell adhesive and osteogenic motifs on the modified Ti screws, which recovered the regular bone metabolism equilibrium between osteogenesis and osteoclastogenesis in an osteoporotic condition. We anticipate that the highly biomimetic peptides and one-step dual-functionalized strategy would provide a facile and effective means for improving the clinical outcome of Ti-based implants in patients with a disturbed bone metabolism.

A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

“…The molecular mechanism of this method derived from mussel foot proteins (e.g., Mytilus edulis foot proteins, Mfps), in which the repetitive catechol residues of DOPA (3,4-dihydroxy-L-phenylalanine) can produce covalent and noncovalent comediated molecular adhesion [14]. A great deal of studies indicates that Mfps-mimics (e.g., polydopamine [15][16][17], DOPA-rich peptides [18,19], and catecholic polymers [20,21]) with catechol groups can adhere stably to virtually all kinds of substrates under wet conditions [22]. In addition, a second-step conjugation with bioactive molecules through amino-or thiolmediated Michael addition allows a variety of biofunctionalizations.…”

Use of Polyphenol Tannic Acid to Functionalize Titanium with Strontium for Enhancement of Osteoblast Differentiation and Reduction of Osteoclast Activity

“…The imbalance in osteoclasts and osteoblasts activity in patients with bone metabolic disease such as osteoporosis can be an obstacle to bone implant success [53,54,55]. Coatings that actively aim at correcting this imbalance are required to recover bone turnover and improve the osseointegration of bone implants [56,57]. We believe that these surface coatings will be beneficial to regulate osteoclast and osteoblast development at implant surfaces.…”

Intraosseous Injection of Calcium Phosphate Polymer-Induced Liquid Precursor Increases Bone Density and Improves Early Implant Osseointegration in Ovariectomized Rats

“…The systemic disease, osteoporosis, seriously impacts the health and quality of life of older adults, and particularly of middle-aged and older women who have experienced menopause. 1 , 2 The prevalence rate of osteoporosis among Chinese women >65 years old is as high as 51.6%. 3 Abnormal bone metabolism caused by osteoporosis results in reduced bone mineral content, destruction of trabecular bone microstructure, inferior bone biomechanical properties and damage to bone regeneration and remodeling.…”