A Versatile Surface Bioengineering Strategy Based on Mussel-Inspired and Bioclickable Peptide Mimic

Abstract

<jats:p>In this work, we present a versatile surface engineering strategy by the combination of mussel adhesive peptide mimicking and bioorthogonal click chemistry. The main idea reflected in this work derived from a novel mussel-inspired peptide mimic with a bioclickable azide group (i.e., DOPA<jats:sub>4</jats:sub>-azide). Similar to the adhesion mechanism of the mussel foot protein (i.e., covalent/noncovalent comediated surface adhesion), the bioinspired and bioclickable peptide mimic DOPA<jats:sub>4</jats:sub>-azide enables stable binding on a broad range of materials, such as metallic, inorganic, and organic polymer substrates. In addition to the material universality, the azide residues of DOPA<jats:sub>4</jats:sub>-azide are also capable of a specific conjugation of dibenzylcyclooctyne- (DBCO-) modified bioactive ligands through bioorthogonal click reaction in a second step. To demonstrate the applicability of this strategy for diversified biofunctionalization, we bioorthogonally conjugated several typical bioactive molecules with DBCO functionalization on different substrates to fabricate functional surfaces which fulfil essential requirements of biomedically used implants. For instance, antibiofouling, antibacterial, and antithrombogenic properties could be easily applied to the relevant biomaterial surfaces, by grafting antifouling polymer, antibacterial peptide, and NO-generating catalyst, respectively. Overall, the novel surface bioengineering strategy has shown broad applicability for both the types of substrate materials and the expected biofunctionalities. Conceivably, the “clean” molecular modification of bioorthogonal chemistry and the universality of mussel-inspired surface adhesion may synergically provide a versatile surface bioengineering strategy for a wide range of biomedical materials.</jats:p>

Photo-functionalized TiO2 nanotubes decorated with multifunctional Ag nanoparticles for enhanced vascular biocompatibility

“…Based on the excellent chemical stability and biosafety [ 1 ], titanium dioxide (TiO 2 ) is a popular medical ceramic with broad application for blood-contacting devices [ 2 , 3 ]. However, like most inorganic materials, TiO 2 has a shortage of insufficient anticoagulant properties [ 4 ].…”

Bioclickable Mussel-Derived Peptides With Immunoregulation for Osseointegration of PEEK

“…Unlike conventional click chemistry (copper-catalyzed alkyne-azide cycloaddition, CuAAC), its reaction is rapid at room temperature and does not require copper ions, which is toxic to most organisms and can cause protein denaturation. The DBCO-azide copper-free click method has been widely used in antibody-peptide preparation, labeling, and conjugation between biomolecules and cells and cell tracking ( Gong et al, 2016 ; Yoon et al, 2016 ; Xiao et al, 2020 ).…”

Assembly of Gentamicin and Zn2+ Loaded Coatings on TiO2 Nanotubes to Synergistically Improve the Blood Compatibility, Endothelial Cell Growth and Antibacterial Activities

“…Generally speaking, ideal medical implants should have the abilities to integrate and communicate with surrounding tissues or cells, trigger speci c cell responses and maintain the function of tissues and organs, and prevent infections caused by microorganisms after the implantation [3,4]. In this regard, surface functionalization represents one of the straightforward and effective methods to endow biomaterials with excellent properties and functions [5,6]. According to the mechanism of the interaction between the implant and the surrounding physiological environment, the introduction of bioactive factors on the surface by physical or chemical conjugation can endow the inert biomaterial with good biological activities, so as to regulate the cellmaterial interaction behaviors, induce speci c cell responses, and prevent the infection caused by implantation and related biological effects [7][8][9][10].…”