Muscle-fiber characteristics in adult mouse-tongue muscles

Abe, Shinichi; Maejima, Masayo; Watanabe, Hiroki; Shibahara, Takahiko; Agematsu, Hiroko; Doi, Tomoyoshi; Sakiyama, Kazuo; Usami, Atsushi; Gojyo, Kazuro; Hashimoto, Masatsugu; Yoshinari, Masao; Ide, Yoshinobu

doi:10.1046/j.0022-7722.2002.00019.x

Cited by 29 publications

(21 citation statements)

References 16 publications

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“…The functional roles of these four isoforms have been clarified. Therefore, the functions of muscles can be elucidated by determining the composition of these four isoforms (1,5,6,10,16,19,23,28) (Table 1). In this study, we examined MyHC-perinatal to determine speed of differentiation of myoblasts, and MyHC-2b, 2d, 2a and 1 to ascertain muscle cell characteristics.…”

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confidence: 99%

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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In vivo studies have shown that changes in the characteristics of skeletal muscle fiber are determined by type of exercise or training. These earlier studies on mechanical stimulation, however, have all employed stimulation applied at a constant intensity, and no studies appear to have investigated change with variation of intensity of stimulation. In this study, we investigated the characteristics and differentiation of myoblasts stretched at different rates. Myoblasts were stimulated at 3 different rates, and the numbers of cells and nuclei on days 1, 3, and 5 were compared. The myosin heavy chain (MyHC) mRNA expression level was also compared. We investigated expression of MyHC-perinatal to determine speed of differentiation of myoblasts, and expression of MyHC2b, 2d, and 2a to ascertain muscle cell characteristics. Counting cells and nuclei of myoblasts revealed clear promotion of differentiation with stretching. With rapid stretching, expression of MyHC-perinatal was high at first, but then showed a decrease. In terms of effect on muscle fiber characteristics, MyHC-2b, MyHC-2d, and MyHC-2a were high with rapid, medium, and slow stretching, respectively. This indicated that myoblast differentiation was promoted regardless of difference in stretching speed, with the myoblasts acquiring the muscle-fiber characteristics appropriate to each rate of stretching.Exercise and training are known to bring about changes in the characteristics of skeletal muscle fibers (26,27). Numerous studies of the human (2,3,4,12,13,17,20,24) have shown that skeletal muscle fiber characteristics change depending on the mode of exercise. Anaerobic exercise facilitates increases in fast-twitch muscle, while aerobic exercise facilitates increases in slow-twitch muscle. Studies using rats have reported that long-term low-intensity exercise increases slow-twitch muscle and decreases fast-twitch muscle (9,15). In this manner, in vivo studies in the rat and the human have shown that exercise changes muscle fiber characteristics. From the perspective of stress, severing a nerve controlling skeletal muscle not only decreases muscle weight, but also changes muscle fiber characteristics (7,14). Studies in which muscle blood flow was regulated using devices such as a tourniquet have shown that even low-intensity training caused marked muscle hypertrophy and increased muscle force (25). However, few in vitro studies have been conducted to support and clarify these findings. In vitro studies have attempted to ascertain the effects of exercise and training by applying different stimuli. The first study (18) removed cells from rat hindlimb and soleus muscle and applied electrical stimuli via a culture solution to measure expression of myosin heavy chain (MyHC) isoforms. In the second study (11), osteoblasts were compressed using glass boards to ascertain morphological and bio-

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Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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“…Reports concerning the expressions of each MyHC isoform in the course of muscle growth and development were mainly about extremities, but recently, reports about head and neck muscles such as masticatory muscle can be seen [5][6][7][8][9][10][11][12][13] . Gojo et al…”

Section: Introductionmentioning

confidence: 99%

“…The tongue muscle consists of fast muscle type 5,15,16) ; thus, MyHC-2b and MyHC-2a, which is considered to have the highest and lowest contractile rates among fast muscle type MyHC isoforms, respectively, were selected to investigate the muscle fiber characteristics 4) . HMGB1 was used as a marker to examine whether the carcinoma of tongue actually developed 18) .…”

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confidence: 99%

Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

Sakiyama

Takizawa

Bando

et al. 2013

J. Hard Tissue Biology.

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It has been suggested that dysphagia caused by excision of carcinoma of the tongue may be affected by the hypofunction of the surrounding muscle tissues, as well as by tissue defects. However, there have been no reports concerning how muscle fiber characteristics change in the regions surrounding the lesion after excision of the carcinoma. In this study, we investigated myosin heavy chain (MyHC) isoforms at genetic levels in order to demonstrate the muscle fiber characteristics of the muscle tissues surrounding lingual carcinoma (the experimental group). High mobility group box 1 (HMGB1) was used as a marker to examine whether tongue cancer actually developed. MyHC-2a expression was high in the experimental group. Thus, the marked changes of muscle fiber characteristics, as seen in this study, were considered to be a biological reaction to changing muscle characteristics for regenerating or maintaining normal muscle tissues against the muscle atrophy caused by carcinoma.

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“…The relationship between the combination and the function of the muscle fibers has been published 1,2) . Skeletal muscles contain two main types of fibers: Slow-twitch or type I and fast-twitch or type II according to their different speeds of contraction 3) .…”

Section: Introductionmentioning

confidence: 99%

HGF and IGF-1 is Present during the Developmental Process of Murine Masseter Muscle

Abe

Nonami

Iwanuma

et al. 2009

J. Hard Tissue Biology.

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Abstract:Until now the developmental process of masticatory muscle (derived from the branchial arches) is not well documented. Thus, in order to clarify the developmental process of murine masseter muscle, Hepatocyte Growth Factor (HGF), Insulin-like Growth Factor-1 (IGF-1) and Myosin Heavy Chain (MyHC) were evaluated by histological examination, western blotting and RT-PCR analyses on embryonic days 14 (E14), 16 (E16) and 18 (E18). E14 showed that the mandibular bone development is initiated by the appearance of the ossification centre in the peripheral zone of Meckel cartilage. Whereas, E16 demonstrated presence of developing masseter muscle at the lower part of the mandible composed of immature bone. On the contrary, E18 showed larger masseter muscle and more mature mandibular than E16. Interestingly, both IGF and HGF proteins and genes increased at initial stage of masseter muscle development, and decreased with the time. A significant change in composition of MyHC was detected in E18 compared with E14 and E16. This result indicate that murine masseter muscle develops in accordance with the surrounding tissue, where IGF-1 and HGF could be an important modulator of stem cell-derived myoblast proliferation and myogenic differentiation, and MyHC-2a and 2d are strongly expressed by mature muscle fibres during the developmental process of the masseter muscle.

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Muscle-fiber characteristics in adult mouse-tongue muscles

Cited by 29 publications

References 16 publications

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

HGF and IGF-1 is Present during the Developmental Process of Murine Masseter Muscle

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