Muscle characteristics in young Norwegian‐Swedish Coldblooded Trotters and associations with breeding index, body size and early training

Revold, Tobias; Ihler, C. F.; Karlström, K.; Larsen, Stig; Essén‐Gustavsson, Birgitta

doi:10.1111/j.2042-3306.2010.00335.x

Cited by 5 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enzyme histochemical methods permitted the observation of the mosaic pattern obtained by reactions and the different fiber types were highlighted, which enabled counting. Such success was also observed in studies of other species, for example, in equines (D´ANGELIS et al, , 2008REVOLD et al, 2011;LINDNER et al, 2013).…”

Section: The Modified Gomori Trichrome (Mgt)supporting

confidence: 48%

Needle muscle biopsy: technique validation and histological and histochemical methods for evaluating canine skeletal muscles

Braga

Padilha

Ferreira

2017

SCA

View full text Add to dashboard Cite

This study evaluated the needle muscle biopsy technique using a 6G Bergström percutaneous needle combined with histological and histochemical methods to analyze the skeletal muscle of dogs. There are few studies about canine skeletal muscles and a lack of reports in the literature about tissue collection and analysis for canine species. Evaluation of 32 German Shepherd samples collected from the gluteus medius, at a depth of 3 cm, was performed. The choice of gluteus medius and the 3-cm depth provided good quantity fragments with sufficient sizes (3-5 mm), which permitted optimal visualization of muscle fibers. Myosin ATPase, at pH 9.4, 4.6, and 4.3, and SDH reactions revealed that all muscle samples analyzed had fibers in the classic mosaic arrangement, enabling counting and typification. The mean percentages of fibers were 29.95% for type I and 70.05% for type II. On the basis of these results, we concluded that the percutaneous needle biopsy technique for canine skeletal muscles is a safe and easy procedure that obtains fragments of proper sizes, thereby enabling the study of muscle fibers. Standardization of the muscle of choice and the depth of muscle sample collection significantly contributed to this success. This is an important method to evaluate muscle fiber types of dogs and diagnose important diseases affecting the skeletal muscles. Key words: Muscle. German shepherd. Diagnosis ResumoO objetivo deste trabalho foi avaliar a técnica de biopsia muscular por agulha de biópsia percutânea tipo Bergström nº seis, e os métodos histológicos e histoquímicos utilizados na análise do músculo esquelético de cães, uma vez que estudos sobre a musculatura esquelética em cães são poucos o que acarreta um número pequeno de relatos na literatura sobre métodos de coleta e análise desse tecido nessa determinada espécie. Foram avaliadas amostras de 32 animais da raça Pastor Alemão coletadas do músculo glúteo médio a uma profundidade de três centímetros. A escolha da profundidade de três centímetros e do músculo Glúteo Médio em cães da raça Pastor Alemão denotou fragmentos colhidos com suficiente viabilidade, bons padrões de tamanho (entre três e cinco milímetros) e boa quantidade e qualidade, permitindo ótima visualização das fibras musculares dos animais da amostra. Nas reações enzimo-histoquímicas da miosina ATPase no pH 9.4, 4.6 e 4.3 e SDH todos os músculos examinados apresentavam a distribuição das fibras musculares com o clássico padrão de mosaico, possibilitando a contagem e a diferenciação dos diferentes tipos. O valor médio de fibras tipo I foi de 29,95% e o valor médio de fibras tipo II, 70,05%. Com base nesses resultados, pode-se concluir que a técnica de biopsia com agulha percutânea utilizada em músculo esquelético de caninos, demonstrou ser um procedimento seguro e de fácil recuperação, obtendo fragmentos de tamanho adequado e, portanto, viabilizando o estudo. A padronização do músculo e da profundidade de coleta da amostra muscular teve contribuição importante. Sendo um importante meio para avaliar ...

show abstract

Section: The Modified Gomori Trichrome (Mgt)supporting

confidence: 48%

Needle muscle biopsy: technique validation and histological and histochemical methods for evaluating canine skeletal muscles

Braga

Padilha

Ferreira

2017

SCA

View full text Add to dashboard Cite

show abstract

“…However, in the present study we found a higher MFT-IIX proportion of the MFT-II fibres. Training affects the MFT proportions of the muscle, with an increase in the MFT-IIA:IIX ratio shown in different breeds [10,35-37]. Therefore, the lower IIA:IIX ratios in these horses compared to the Thoroughbred racehorses was likely to be due to the reduced training experienced by the horses in the present study, as they had only had light exercise prior to euthanasia.…”

Section: Discussionmentioning

confidence: 76%

Muscle fibre type distribution of the thoracolumbar and hindlimb regions of horses: relating fibre type and functional role

et al. 2014

View full text Add to dashboard Cite

BackgroundAlthough the majority of equine muscles have a mixed fibre type distribution indicative of diverse functional roles, the predominance of a fibre type can indicate the primary function of a muscle. The deep epaxial musculature has an important role in core spinal stability in humans, reflected as a predominantly muscle fibre type (MFT) I or postural fibre type. The fibre type of the deep epaxial musculature has not been determined in horses. The objective of the study was to determine the MFT distribution in selected muscles of thoracolumbar and hindlimb region of horses. This included deep epaxial and hypaxial muscles that were hypothesised to have a postural stabilising role. A second objective was to examine differences in MFT distribution between horses bred for endurance (Arabian) and sprinting (Quarter horse). Muscle biopsy samples were obtained from selected thoracolumbar and hind limb muscles of 5 Quarter horses, 4 Arabians, and 2 Thoroughbreds. The myosin heavy chain distribution was determined by gel electrophoresis. Mann–Whitney rank test was used to compare the proportional MFT and differences between breeds.ResultsMm. sacrocaudalis dorsalis medialis and diaphragm had the highest proportion of MFT-I. The remaining deep epaxial muscles and the hypaxial muscle m. psoas minor had approximately equal MFT I and II proportions. Mm. psoas major, iliocostalis, longissimus dorsi and the hind limb muscles contained mostly MFT-IIX. The fibre type distribution was similar between Arabians and Quarter horses, although Quarter horses had more MFT-IIX fibres in psoas major (P = 0.02) while Arabians had more MFT-I fibres in m. longissimus dorsi (P = 0.03).ConclusionsThe fibre type distribution of the deep epaxial muscles, mm psoas minor and diaphragm varied from approximately equal MFT-I and II proportions to predominantly MFT-I suggesting a postural stabilising role possibly important in core spinal stability. In contrast the fibre type proportions of mm psoas major, iliocostalis, longissimus dorsi and the hind limb muscles were mainly MFT-II suggesting a locomotory role. Knowledge of fibre type distribution in such a clinically important area can direct diagnosis, prevention and treatment of muscular or neuromotor dysfunction.

show abstract

“…However, in the present study we found a higher MFT-IIX proportion of the MFT-II fibres. Training affects the MFT proportions of the muscle, with an increase in the MFT-IIA:IIX ratio shown in different breeds [10,[35][36][37]. Therefore, the lower IIA:IIX ratios in these horses compared to the Thoroughbred racehorses was likely to be due to the reduced training experienced by the horses in the present study, as they had only had light exercise prior to euthanasia.…”

Section: Discussionmentioning

confidence: 77%

Muscle Fibre Type Distribution of the Thoracolumbar and Hindlimb Regions of Horses: Relating Fibre Type and Functional Role

Hyytiäinen

Mykkänen

Hielm‐Björkman

et al. 2014

Equine Veterinary Journal

View full text Add to dashboard Cite

Background: Although the majority of equine muscles have a mixed fibre type distribution indicative of diverse functional roles, the predominance of a fibre type can indicate the primary function of a muscle. The deep epaxial musculature has an important role in core spinal stability in humans, reflected as a predominantly muscle fibre type (MFT) I or postural fibre type. The fibre type of the deep epaxial musculature has not been determined in horses. The objective of the study was to determine the MFT distribution in selected muscles of thoracolumbar and hindlimb region of horses. This included deep epaxial and hypaxial muscles that were hypothesised to have a postural stabilising role. A second objective was to examine differences in MFT distribution between horses bred for endurance (Arabian) and sprinting (Quarter horse). Muscle biopsy samples were obtained from selected thoracolumbar and hind limb muscles of 5 Quarter horses, 4 Arabians, and 2 Thoroughbreds. The myosin heavy chain distribution was determined by gel electrophoresis. Mann-Whitney rank test was used to compare the proportional MFT and differences between breeds. Results: Mm. sacrocaudalis dorsalis medialis and diaphragm had the highest proportion of MFT-I. The remaining deep epaxial muscles and the hypaxial muscle m. psoas minor had approximately equal MFT I and II proportions. Mm. psoas major, iliocostalis, longissimus dorsi and the hind limb muscles contained mostly MFT-IIX. The fibre type distribution was similar between Arabians and Quarter horses, although Quarter horses had more MFT-IIX fibres in psoas major (P = 0.02) while Arabians had more MFT-I fibres in m. longissimus dorsi (P = 0.03).

show abstract

Muscle characteristics in young Norwegian‐Swedish Coldblooded Trotters and associations with breeding index, body size and early training

Abstract: Muscle characteristics differ among young NSCT horses, but the innate oxidative capacity seems to be lower than in Standardbreds of comparable age. Early training may influence on these parameters.

Cited by 5 publications

References 33 publications

Needle muscle biopsy: technique validation and histological and histochemical methods for evaluating canine skeletal muscles

Needle muscle biopsy: technique validation and histological and histochemical methods for evaluating canine skeletal muscles

Muscle fibre type distribution of the thoracolumbar and hindlimb regions of horses: relating fibre type and functional role

Muscle Fibre Type Distribution of the Thoracolumbar and Hindlimb Regions of Horses: Relating Fibre Type and Functional Role

Contact Info

Product

Resources

About