The objective of this study was to determine and confirm the percentage of type I and type II muscle fibers that comprise the Gluteus Medius muscle in male and female canines of the German Shepherd breed, with standardized care, in different age groups, using the enzyme histochemical method. Muscle samples were collected from the Gluteus Medius muscles of forty clinically healthy dogs of the German Shepherd breed using the technique of percutaneous needle muscle biopsy. The samples were evaluated using histological and enzyme histochemical methods. The percentages of type I and II fibers and the ratio between the quantity of type I fibers/quantity of type II fibers were evaluated using the parameters of weight, age group, correlation between sex and age group, and between the sexes. It was found that there was no significant difference in relation to the types of fibers for the parameters of weight, age group, and age of the females. The correlation between the ages of the males suggested an increase in the percentage of type I fibers, a decrease in the percentage of type II fibers, or an increase in the ratio during the aging process. It was concluded that there was a decrease in the percentage of type II fibers with advancing age in male dogs, but without significant difference in the percentage of type I and type II fibers in relation to the weight. Anat Rec, 299:1540-1547, 2016. © 2016 Wiley Periodicals, Inc.
This study evaluated the needle muscle biopsy technique using a 6G Bergström percutaneous needle combined with histological and histochemical methods to analyze the skeletal muscle of dogs. There are few studies about canine skeletal muscles and a lack of reports in the literature about tissue collection and analysis for canine species. Evaluation of 32 German Shepherd samples collected from the gluteus medius, at a depth of 3 cm, was performed. The choice of gluteus medius and the 3-cm depth provided good quantity fragments with sufficient sizes (3-5 mm), which permitted optimal visualization of muscle fibers. Myosin ATPase, at pH 9.4, 4.6, and 4.3, and SDH reactions revealed that all muscle samples analyzed had fibers in the classic mosaic arrangement, enabling counting and typification. The mean percentages of fibers were 29.95% for type I and 70.05% for type II. On the basis of these results, we concluded that the percutaneous needle biopsy technique for canine skeletal muscles is a safe and easy procedure that obtains fragments of proper sizes, thereby enabling the study of muscle fibers. Standardization of the muscle of choice and the depth of muscle sample collection significantly contributed to this success. This is an important method to evaluate muscle fiber types of dogs and diagnose important diseases affecting the skeletal muscles. Key words: Muscle. German shepherd. Diagnosis ResumoO objetivo deste trabalho foi avaliar a técnica de biopsia muscular por agulha de biópsia percutânea tipo Bergström nº seis, e os métodos histológicos e histoquímicos utilizados na análise do músculo esquelético de cães, uma vez que estudos sobre a musculatura esquelética em cães são poucos o que acarreta um número pequeno de relatos na literatura sobre métodos de coleta e análise desse tecido nessa determinada espécie. Foram avaliadas amostras de 32 animais da raça Pastor Alemão coletadas do músculo glúteo médio a uma profundidade de três centímetros. A escolha da profundidade de três centímetros e do músculo Glúteo Médio em cães da raça Pastor Alemão denotou fragmentos colhidos com suficiente viabilidade, bons padrões de tamanho (entre três e cinco milímetros) e boa quantidade e qualidade, permitindo ótima visualização das fibras musculares dos animais da amostra. Nas reações enzimo-histoquímicas da miosina ATPase no pH 9.4, 4.6 e 4.3 e SDH todos os músculos examinados apresentavam a distribuição das fibras musculares com o clássico padrão de mosaico, possibilitando a contagem e a diferenciação dos diferentes tipos. O valor médio de fibras tipo I foi de 29,95% e o valor médio de fibras tipo II, 70,05%. Com base nesses resultados, pode-se concluir que a técnica de biopsia com agulha percutânea utilizada em músculo esquelético de caninos, demonstrou ser um procedimento seguro e de fácil recuperação, obtendo fragmentos de tamanho adequado e, portanto, viabilizando o estudo. A padronização do músculo e da profundidade de coleta da amostra muscular teve contribuição importante. Sendo um importante meio para avaliar ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.