Muscle-Based Gene Therapy and Tissue Engineering for Treatment of Growth Plate Injuries

Lee, Chang Woo; Martinek, V.; Ūsas, Arvydas; Musgrave, Douglas S.; Pickvance, E. A.; Robbins, Paul D.; Moreland, Morey S.; Fu, Freddie H.; Huard, Johnny

doi:10.1097/01241398-200209000-00001

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…IGF‐I is a polypeptide hormone that regulates chondrocyte activity (Trippel et al ., ). Although the protein has not been used for growth plate regeneration, adenoviral‐mediated gene therapy with IGF‐I prevented closure of the growth plate (Lee et al ., ). Furthermore, IGF‐I delivery has shown great promise for articular cartilage repair in large mammals (Fortier et al ., ), and IGF‐I is used clinically in children (Midyett et al ., ), potentially making the pathway for translation to the clinic easier.…”

Section: Introductionmentioning

confidence: 97%

“…This latter approach, however, requires a second procedure and damage to healthy tissue. All these treatment have inadequacies, leaving approximately 50% of patients with continuing deformities (Hasler and Foster, ; Lee et al ., ). Currently, there is no treatment for growth plate injury that leads to complete regeneration of the damaged tissue.…”

Section: Introductionmentioning

confidence: 99%

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Treatment of growth plate injury using IGF-I-loaded PLGA scaffolds

Sundararaj

Cieply

Gupta

et al. 2012

J Tissue Eng Regen Med

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Growth plate fracture can lead to retarded growth and unequal limb length, which may have a lifelong effect on a person's physical stature. The goal of this research was to develop an in vivo tissue-engineering approach for the treatment of growth plate injury via localized delivery of insulin-like growth factor I (IGF-I) from cell-free poly(lactic-co-glycolic acid) (PLGA) scaffolds. Mass loss and drug release studies were conducted to study the scaffold degradation and IGF-I release patterns. In vitro cell studies showed that rat bone marrow stromal cells seeded on the porous scaffolds colonized the pores and deposited matrix within the scaffolds. These in vitro evaluations were followed by a proof-of-concept animal study involving implantation of scaffolds in proximal tibial growth plate defects in New Zealand white rabbits. Histological analysis of tissue sections from the in vivo studies showed regeneration of cartilage, albeit with disorganized structure, at the site of implantation of IGF-I-releasing scaffolds; in contrast, only bone was formed in empty defects and those treated with IGF-free scaffolds. The present findings show the potential for treating growth plate injury using in vivo tissue engineering techniques.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Treatment of growth plate injury using IGF-I-loaded PLGA scaffolds

Sundararaj

Cieply

Gupta

et al. 2012

J Tissue Eng Regen Med

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“…The potential effects of IGF-I and BMP-2 on physeal bar formation have been reported by Lee et al [58]. In this animal study, genetically altered muscle tissue was used as an interposition material.…”

Section: Pathogenesis Of Physeal Bar Formationmentioning

confidence: 92%

“…This study showed that muscle tissue with the IGF-1 gene can prevent the formation of bars in the growth plate and has a protective effect on the chondrocytes in the growth plate. In contrast, muscle tissue with BMP-2 gene transfer alone has been shown to increase osteogenic activity in the growth plate [58].…”

Section: Autologous Muscle Interpositionmentioning

confidence: 97%

The normal and fractured physis: an anatomic and physiologic overview

Hosseinzadeh

Milbrandt

2016

Journal of Pediatric Orthopaedics B

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The growth plate (physis) is responsible for enabling and regulating longitudinal growth of upper and lower limbs. This regulation occurs through interaction of the cells in the growth plate with systemic and locally produced factors. This complex interaction leads to precisely controlled changes in chondrocyte size, receptors, and matrix, which ultimately result in endochondral bone formation. With advances in cellular and molecular biology, our knowledge about these complex interactions has increased significantly over the past decade. Deficiency of any of the regulating factors or physeal injury during childhood can alter this well-orchestrated sequence of events and lead to abnormalities in growth. This review highlights the histology of the normal physis, including recent findings at the cellular and molecular levels, mechanics and mechanobiology of the growth plate, pathologies that can affect the physis, and treatment options, including interposition materials.

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“…In another meritorious approach, adenovirus-mediated gene transfer of IGF-1 into an autologous muscle scaffold showed a favorable effect on the restoration of the injured growth plate ( Fig. 6A) (Lee et al, 2002). By contrast, the release of BMP-2 after adenoviral BMP-2 gene transfer caused an increase in osteogenic activity, and consequently a premature closure of the growth plate ( Fig.…”

Section: Tissue-engineered Epiphyseal Growth Platementioning

confidence: 99%

Stem cell-based composite tissue constructs for regenerative medicine

Rahaman

Mao

2005

Biotechnol. Bioeng.

160

122

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Abstract:A major task of contemporary medicine and dentistry is restoration of human tissues and organs lost to diseases and trauma. A decade-long intense effort in tissue engineering has provided the proof of concept for cellbased replacement of a number of individual tissues such as the skin, cartilage, and bone. Recent work in stem cellbased in vivo restoration of multiple tissue phenotypes by composite tissue constructs such as osteochondral and fibro-osseous grafts has demonstrated probable clues for bioengineered replacement of complex anatomical structures consisting of multiple cell lineages such as the synovial joint condyle, tendon-bone complex, bone-ligament junction, and the periodontium. Of greater significance is a tangible contribution by current attempts to restore the structure and function of multitissue structures using cellbased composite tissue constructs to the understanding of ultimate biological restoration of complex organs such as the kidney or liver. The present review focuses on recent advances in stem cell-based composite tissue constructs and attempts to outline challenges for the manipulation of stem cells in tailored biomaterials in alignment with approaches potentially utilizable in regenerative medicine of human tissues and organs. B

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Muscle-Based Gene Therapy and Tissue Engineering for Treatment of Growth Plate Injuries

Cited by 9 publications

References 34 publications

Treatment of growth plate injury using IGF-I-loaded PLGA scaffolds

Treatment of growth plate injury using IGF-I-loaded PLGA scaffolds

The normal and fractured physis: an anatomic and physiologic overview

Stem cell-based composite tissue constructs for regenerative medicine

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