Muscarinic receptors coupled to phosphoinositide hydrolysis and elevated cytosolic calcium in a human neuroblastoma cell line SK‐N‐SH

Baird, John G.; Lambert, David G.; McBain, J. B.; Nahorski, Stefan R.

doi:10.1111/j.1476-5381.1989.tb12681.x

Cited by 16 publications

(10 citation statements)

References 19 publications

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“…, 2008). This result is similar to a comparison of both assays used in carbachol‐treated animal cells in which Ins(1,4,5)P 3 was noted to increase in both types of assay, although the radiolabeling showed a greater overall Ins(1,4,5)P 3 increase (Baird et al. , 1989; Lambert et al.…”

Section: The Controversial Roles Of Dag and Ins(145)p3 In The Plsupporting

confidence: 85%

The cellular language ofmyo‐inositol signaling

2011

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SummaryThe simple polyol, myo-inositol, is used as a building block of a cellular language that plays various roles in signal transduction. This review describes the terminology used to denote myo-inositol-containing molecules, with an emphasis on how phosphate and fatty acids are added to create second messengers used in signaling. Work in model systems has delineated the genes and enzymes required for synthesis and metabolism of many myo-inositol-containing molecules, with genetic mutants and measurement of second messengers playing key roles in developing our understanding. There is increasing evidence that molecules such as myo-inositol(1,4,5)trisphosphate and phosphatidylinositol(4,5)bisphosphate are synthesized in response to various signals plants encounter. In particular, the controversial role of myo-inositol(1,4,5)trisphosphate is addressed, accompanied by a discussion of the multiple enzymes that act to regulate this molecule. We are also beginning to understand new connections of myo-inositol signaling in plants. These recent discoveries include the novel roles of inositol phosphates in binding to plant hormone receptors and that of phosphatidylinositol(3)phosphate binding to pathogen effectors.

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Section: The Controversial Roles Of Dag and Ins(145)p3 In The Plsupporting

confidence: 85%

The cellular language ofmyo‐inositol signaling

2011

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“…Indeed, phosphorylation of a number of PLC-linked receptors has been reported (Klueppelberg et al, 1992;Kwatra et al, 1993) including that of the m3 receptor by PKC and an additional kinase distinct from either protein kinase A (PKA), Ca2+-calmodulin-dependent kinase or P-ARK . (Yu & Sadee, 1986) or SH-SY5Y cell lines (Ogino & Costa, 1993 (Yu & Sadee, 1986;Fisher & Heacock, 1988;Fisher et al, 1989;Baird et al, 1989) and SH-SY5Y cells (Lambert & Nahorski, 1990b;Lambert et al, 1991 (Kenakin, 1987 [Ca2J]i is an 'all-or-none' response (Bootman et al, 1992;Shao & McCarthy, 1993 release whereby the release of a small amount of Ca2l initiates the release of further Ca2" from ryanodine-sensitive stores (see Putney & Bird, 1993 receptor activation (Bezprozvanny et al, 1991 all-or-none phenomenon. Whatever the mechanism, the potency difference defines a step which features as an important amplification stage within the signal transduction pathway.…”

Section: Discussionmentioning

confidence: 99%

“…This was applied to a Dowex AGlX8, 200-400 mesh column which was then washed with 10 ml of 25 mM ammonium formate. Inositol phosphates were washed off with 10 ml of IM ammonium formate/0.1 M formic acid (Baird et al, 1989). A 2 ml aliquot of this was counted by liquid scintillation spectrometry following addition of 8 ml Floscint.…”

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confidence: 99%

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Willars

Nahorski

1995

British J Pharmacology

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Road, Leicester LEI 9HN 1 Muscarinic and bradykinin receptor-mediated Ins(1,4,5)P3 accumulation, Ca2+ mobilization and Ca2+ entry have been examined in human SH-SY5Y neuroblastoma cells. This has allowed both direct comparison of signalling events by two receptor types potentially linked to the same transduction pathway and an investigation of the interactions between the components of this pathway. 2 Stimulation of muscarinic receptors with carbachol produced biphasic accumulations of Ins(1,4,5)P3 consisting of a rapid peak followed by a lower sustained phase. Both phases were dose-dependent but the potency of elevation at peak was significantly less than that of the sustained phase. Bradykinin also dose-dependently stimulated Ins(1,4,5)P3 accumulation but responses were smaller and not sustained. the ability of agonists for predominantly sustained PLC activation, potentially driven by a partially desensitized receptor, as opposed to rapid activation by a fully sensitized receptor. 8 These data provide quantitative comparisons between and within components of the receptormediated phosphoinositide and Ca24 signalling pathway, provide mechanistic insights into regulation of these components and characterize a model system in which heterologous interaction between two receptors linked to the same transduction pathway may be examined.

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“…Samples were applied to a Dowex AG1X8, 200–400 mesh column which was washed with 10 ml of 25 m M ammonium formate. Inositol phosphates were eluted with 10 ml of 1 M ammonium formate/0.1 M formic acid ( Baird et al ., 1989 ). A 5 ml aliquot of this was counted by liquid scintillation spectrometry.…”

Section: Methodsmentioning

confidence: 99%

Complex relationship between Ins(1,4,5)P₃ accumulation and Ca²⁺‐signalling in a human neuroblastoma revealed by cellular differentiation

Martin

Nahorski

Willars

1999

British J Pharmacology

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1 Dierentiation of SH-SY5Y neuroblastoma cells induces morphological and biochemical changes consistent with a more neuronal phenotype. These cells may therefore provide a model for studying phenomena such as signal transduction in a neuronal context whilst retaining the advantages of a homogenous cell population expressing a well characterized array of G-protein coupled receptors. 2 This study examined the eects of dierentiating SH-SY5Y cells on muscarinic-and bradykininreceptor-mediated phosphoinositide and Ca 2+ signalling. Retinoic acid (10 mM, 6 days) along with a lowered serum concentration produced phenotypic changes consistent with dierentiation including reduced proliferation and increased neurite outgrowth. 3 Dierentiation increased the magnitude and potency of rapid Ins(1,4,5)P 3 responses to a full muscarinic receptor agonist. Bradykinin receptor-mediated Ins(1,4,5)P 3 signalling was also potentiated following dierentiation. Determination of agonist-evoked accumulation of [ 3 H]-inositol phosphates under lithium-block demonstrated these changes re¯ected enhanced phospholipase C activity which is consistent with observed increases in the expression of muscarinic and bradykinin receptors. 4 Despite the marked alterations in Ins(1,4,5)P 3 signalling following dierentiation, elevations of intracellular [Ca 2+ ] were totally unaltered. Thus, in SH-SY5Y cells, the relationship between the elevations of Ins(1,4,5)P 3 and intracellular [Ca 2+ ] is agonist dependent and aected by the state of dierentiation. This demonstrates that mechanisms other than the measured increase in Ins(1,4,5)P 3 regulate the elevation of intracellular [Ca 2+ ].

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Muscarinic receptors coupled to phosphoinositide hydrolysis and elevated cytosolic calcium in a human neuroblastoma cell line SK‐N‐SH

Cited by 16 publications

References 19 publications

The cellular language ofmyo‐inositol signaling

The cellular language ofmyo‐inositol signaling

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Complex relationship between Ins(1,4,5)P₃ accumulation and Ca²⁺‐signalling in a human neuroblastoma revealed by cellular differentiation

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Muscarinic receptors coupled to phosphoinositide hydrolysis and elevated cytosolic calcium in a human neuroblastoma cell line SK‐N‐SH

Cited by 16 publications

References 19 publications

The cellular language ofmyo‐inositol signaling

The cellular language ofmyo‐inositol signaling

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells

Complex relationship between Ins(1,4,5)P3 accumulation and Ca2+‐signalling in a human neuroblastoma revealed by cellular differentiation

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Product

Resources

About

Quantitative comparisons of muscarinic and bradykinin receptor‐mediated Ins (1,4,5)P₃ accumulation and Ca²⁺ signalling in human neuroblastoma cells

Complex relationship between Ins(1,4,5)P₃ accumulation and Ca²⁺‐signalling in a human neuroblastoma revealed by cellular differentiation