Murine Neural Stem Cell (NSC) Line, MEB5-derived Decellularized Matrix as an <i>In Vitro</i> Extracellular Matrix Model in NSC Niche

Hoshiba, Takashi; Sugano, Yuki; Yokoyama, Natsumi

doi:10.1246/cl.180788

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…In contrast to the TS-ECM, which is the perfect scaffolding substance for tissue engineering, the CECM is usually a place where primary culture cells or mesenchymal stem cells (MSCs) can be regenerated while maintaining their differentiation and potential for proliferation [ 61 , 66 , 67 ]. The CECM can regenerate bone marrow-derived MSCs [ 68 , 69 ], neural precursor cells [ 70 ], and periodontal ligament stem cells [ 71 ] and can enhance the differentiation and proliferation potential of primary cells such as chondrocytes [ 72 , 73 ], myeloid cells [ 74 , 75 ], and hepatocytes [ 76 ]. This ability is mainly due to anti-inflammatory and antioxidant effects [ 73 , 77 ], although the underlying mechanisms require further investigation.…”

Section: Ecm and Decm Overviewmentioning

confidence: 99%

Functional acellular matrix for tissue repair

Wang¹,

Tang²,

Yang³

et al. 2023

Materials Today Bio

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Section: Ecm and Decm Overviewmentioning

confidence: 99%

Functional acellular matrix for tissue repair

Wang¹,

Tang²,

Yang³

et al. 2023

Materials Today Bio

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“…Compared with TS-ECM, an ideal scaffold material in tissue engineering, C-ECM is normally considered an in vitro niche, in which primary cells and MSCs can be rejuvenated to maintain their proliferation and differentiation capacity [112][113][114]. For instance, C-ECM has been demonstrated to refresh tissue-specific stem cells such as synovium-derived stem cells (SDSCs) [115][116][117][118][119][120][121][122], bone marrow-derived MSCs (BMSCs) [123][124][125], umbilical cord-derived MSCs (UCMSCs) [126,127], infrapatellar fat pad-derived stem cells (IPFSCs) [128][129][130], ESCs [131], periodontal ligament stem cells [132], and neural progenitor cells [133]. C-ECM also refreshes primary cells such as chondrocytes [134,135], nucleus pulposus cells [136,137], and hepatic cells [138] in proliferation and redifferentiation capacities (Table 4).…”

Section: Cell-derived Ecmmentioning

confidence: 99%

Decellularized extracellular matrix mediates tissue construction and regeneration

Liu

Pei

et al. 2021

Front. Med.

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Contributing to organ formation and tissue regeneration, extracellular matrix (ECM) constituents provide tissue with three-dimensional (3D) structural integrity and cellular-function regulation. Containing the crucial traits of the cellular microenvironment, ECM substitutes mediate cell—matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo. However, these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures. Cultured cells also produce rich ECM, particularly stromal cells. Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well. Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. Moreover, current preclinical applications by which ECM components modulate the wound-healing process are reviewed.

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“…Various studies demonstrated that MSC-derived ECM can recapitulate the stem cell niche sufficiently to protect reseeded MSCs from oxidative stress, promote their proliferation, and conserve their stemness (Chen et al, 2007;Lai et al, 2010;Liu et al, 2016;Xing et al, 2020). CD-ECMs were also shown to maintain the native phenotype of neural progenitor cells (Yang et al, 2017;Hoshiba et al, 2018), embryonic stem cells (ESCs) (Klimanskaya et al, 2005), periodontal ligament stem cells (Xiong et al, 2019) and hematopoietic stem cells (Prewitz et al, 2013). Furthermore, ECMs derived from younger MSCs were shown FIGURE 2 | CD-ECM applications in fundamental research, pathophysiological studies and renegerative medicine.…”

Section: Stem Cell Nichesmentioning

confidence: 99%

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

Assunção

Dehghan-Baniani

Yiu

et al. 2020

Front. Bioeng. Biotechnol.

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Cell-derived extracellular matrices (CD-ECMs) captured increasing attention since the first studies in the 1980s. The biological resemblance of CD-ECMs to their in vivo counterparts and natural complexity provide them with a prevailing bioactivity. CD-ECMs offer the opportunity to produce microenvironments with costumizable biological and biophysical properties in a controlled setting. As a result, CD-ECMs can improve cellular functions such as stemness or be employed as a platform to study cellular niches in health and disease. Either on their own or integrated with other materials, CD-ECMs can also be utilized as biomaterials to engineer tissues de novo or facilitate endogenous healing and regeneration. This review provides a brief overview over the methodologies used to facilitate CD-ECM deposition and manufacturing. It explores the versatile uses of CD-ECM in fundamental research and therapeutic approaches, while highlighting innovative strategies. Furthermore, current challenges are identified and it is accentuated that advancements in methodologies, as well as innovative interdisciplinary approaches are needed to take CD-ECM-based research to the next level.

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Murine Neural Stem Cell (NSC) Line, MEB5-derived Decellularized Matrix as an In Vitro Extracellular Matrix Model in NSC Niche

Cited by 6 publications

References 18 publications

Functional acellular matrix for tissue repair

Functional acellular matrix for tissue repair

Decellularized extracellular matrix mediates tissue construction and regeneration

Cell-Derived Extracellular Matrix for Tissue Engineering and Regenerative Medicine

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