Murine Hox-1.11 homeobox gene structure and expression.

Tan, Dong-Ping; Ferrante, J.; Nazarali, Adil J.; Shao, Xiaoping; Kozak, Christine A.; Guo, Vicky; Nirenberg, Marshall W.

doi:10.1073/pnas.89.14.6280

Cited by 51 publications

(23 citation statements)

References 25 publications

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“…Immediately upstream of the gene HoxA2 we found a 352-bp region that is 85% conserved that constitutes part of the HoxA2 promoter described by Tan et al (1992) in the mouse HoxA cluster. The Krx20 element and the nearby "box a", described by Nonchev et al (1996) as being involved in HoxA2 trans-activation in mouse, and present in tilapia HoxA cluster (Fig.…”

Section: Description Of Some Putative Regulatory Elementsmentioning

confidence: 99%

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Evolutionary Conservation of Regulatory Elements in Vertebrate Hox Gene Clusters

Santini¹,

Boore²,

Meyer³

2003

Genome Res.

135

115

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Comparisons of DNA sequences among evolutionarily distantly related genomes permit identification of conserved functional regions in noncoding DNA. Hox genes are highly conserved in vertebrates, occur in clusters, and are uninterrupted by other genes. We aligned (PipMaker) the nucleotide sequences of the HoxA clusters of tilapia, pufferfish, striped bass, zebrafish, horn shark, human, and mouse, which are separated by approximately 500 million years of evolution. In support of our approach, several identified putative regulatory elements known to regulate the expression of Hox genes were recovered. The majority of the newly identified putative regulatory elements contain short fragments that are almost completely conserved and are identical to known binding sites for regulatory proteins (Transfac database). The regulatory intergenic regions located between the genes that are expressed most anteriorly in the embryo are longer and apparently more evolutionarily conserved than those at the other end of Hox clusters. Different presumed regulatory sequences are retained in either the A␣ or A␤ duplicated Hox clusters in the fish lineages. This suggests that the conserved elements are involved in different gene regulatory networks and supports the duplication-deletioncomplementation model of functional divergence of duplicated genes.

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Section: Description Of Some Putative Regulatory Elementsmentioning

confidence: 99%

“…Promoters are located immediately 5Ј upstream of genes (e.g., HoxA2 promoter; Tan et al 1992) and RAREs are located 3Ј of the regulated gene (e.g., Frasch et al 1995). However, the largest part of conserved regions we found is located between two genes and is quite distant (by 1-5 kb; column 1 in Table 2) from both.…”

Section: Search For Regulatory Sequencesmentioning

confidence: 99%

Evolutionary Conservation of Regulatory Elements in Vertebrate Hox Gene Clusters

Santini¹,

Boore²,

Meyer³

2003

Genome Res.

135

115

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“…GCA GCC CGA GAA AAG GAA CTG GTG AAT GTG AAA AAG GGA ACA CTT CTT CCA TCA GAG CTG TCA GGA ATT GGT GCA 1024 1.4 (70), Hox-1.11 (48,65), and rat homeobox Rlb (25). Three clones represented the cDNA designated Gax.…”

Section: Methodsmentioning

confidence: 99%

“…It is similar to the Drosophila homeotic geneproboscipedia (20), which is identical to the Gax homeodomain in 40 of 61 amino acid residues (66% identity); Hox-i. ii, with 39 of 61 amino acids being identical (64% identity) (65); and the recently reported Chlorohydra viridissima gene Cnox2, with 37 of 61 amino acids being identical (61% identity) (56). (Fig.…”

Section: Mlabngate*r E-krq---y-rh--l---k--hynr----r--i---ht-v-s---i-imentioning

confidence: 99%

Molecular cloning of a diverged homeobox gene that is rapidly down-regulated during the G0/G1 transition in vascular smooth muscle cells.

Gorski¹,

LePage²,

Patel³

et al. 1993

Mol. Cell. Biol.

131

107

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Adult vascular smooth muscle cells dedifferentiate and reenter the cell cycle in response to growth factor stimulation. Here we describe the molecular cloning from vascular smooth muscle, the structure, and the chromosomal location of a diverged homeobox gene, Gax, whose expression is largely confined to the cardiovascular tissues of the adult. In quiescent adult rat vascular smooth muscle cells, Gax mRNA levels are down-regulated as much as 15-fold within 2 h when these cells are induced to proliferate with platelet-derived growth factor (PDGF) or serum growth factors. This reduction in Gax mRNA is transient, with levels beginning to rise between 8 and 24 h after mitogen stimulation and returning to near normal by 24 to 48 h. The Gax down-regulation is dose dependent and can be correlated with the mitogen's ability to stimulate DNA synthesis. PDGF-AA, a weak mitogen for rat vascular smooth muscle cells, did not affect Gax transcript levels, while PDGF-AB and -BB, potent mitogens for these cells, were nearly as effective as fetal bovine serum. The removal of serum from growing cells induced Gax expression fivefold within 24 h. These data suggest that Gax is likely to have a regulatory function in the G0-to-G1 transition of the cell cycle in vascular smooth muscle cells.

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“…The hybridization conditions were as described in ref. 7. One Hoxd-3 genomic DNA clone (clone AT22) was obtained.…”

Section: Methodsmentioning

confidence: 99%

Sequence and expression of the murine Hoxd-3 homeobox gene.

Tan

Shao²,

Pu³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Murine Hoxd-3 (Hox 4.1) genomic DNA and cDNA and Hoxa-3 (Hox 1.5) cDNA were cloned and sequenced. The homeodomains of Hoxd-3 and Hoxa-3 and regions before and after the homeodomain are highly conserved. Both Hoxd-3 and Hoxa-3 proteins have a proline-rich region that contains consensus amino acid sequences for binding to Src homology 3 domains of some signal transduction proteins. Northern blot analysis of RNA from 8-to 11-day-old mouse embryos revealed a 4.3-kb species of Hoxd-3 RNA, whereas a less abundant 3.0-kb species of Hoxd-3 RNA was found in RNA from 9-to 11-day-old embryos. Two species of Hoxd-3 poly(A) + RNA, 4.3 and 6.0 kb in length, were found in poly(A)

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Murine Hox-1.11 homeobox gene structure and expression.

Cited by 51 publications

References 25 publications

Evolutionary Conservation of Regulatory Elements in Vertebrate Hox Gene Clusters

Evolutionary Conservation of Regulatory Elements in Vertebrate Hox Gene Clusters

Molecular cloning of a diverged homeobox gene that is rapidly down-regulated during the G0/G1 transition in vascular smooth muscle cells.

Sequence and expression of the murine Hoxd-3 homeobox gene.

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