Sequence and expression of the murine Hoxd-3 homeobox gene.

Tan, Dong-Ping; Shao, Xiaoping; Pu, Le-Ping; Guo, Vicky; Nirenberg, Marshall W.

doi:10.1073/pnas.93.16.8247

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The genes contain a homeobox, a 183-bp sequence encoding a 61-amino acid sequence that has a DNA-binding motif for transcriptional regulation of sofar nondefined genes. Some of the homologs of Hox genes that exist in Drosophila are expressed in the meso-and metane-phros, such as Hoxd-3 (263). Although Hox genes are believed to be involved primarily in regional patterning during embryogenesis, their specific roles in kidney organogenesis remain to be discovered, since very few of the targeted mutations so far have resulted in renal defects (149), suggesting redundant gene functions in the cluster or nested expression domains.…”

Section: Homeobox Genesmentioning

confidence: 99%

Embryonic Renal Epithelia: Induction, Nephrogenesis, and Cell Differentiation

Horster

Braun

Huber

1999

Physiological Reviews

184

147

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Embryonic metanephroi, differentiating into the adult kidney, have come to be a generally accepted model system for organogenesis. Nephrogenesis implies a highly controlled series of morphogenetic and differentiation events that starts with reciprocal inductive interactions between two different primordial tissues and leads, in one of two mainstream processes, to the formation of mesenchymal condensations and aggregates. These go through the intricate process of mesenchyme-to-epithelium transition by which epithelial cell polarization is initiated, and they continue to differentiate into the highly specialized epithelial cell populations of the nephron. Each step along the developmental metanephrogenic pathway is initiated and organized by signaling molecules that are locally secreted polypeptides encoded by different gene families and regulated by transcription factors. Nephrogenesis proceeds from the deep to the outer cortex, and it is directed by a second, entirely different developmental process, the ductal branching of the ureteric bud-derived collecting tubule. Both systems, the nephrogenic (mesenchymal) and the ductogenic (ureteric), undergo a repeat series of inductive signaling that serves to organize the architecture and differentiated cell functions in a cascade of developmental gene programs. The aim of this review is to present a coherent picture of principles and mechanisms in embryonic renal epithelia.

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Section: Homeobox Genesmentioning

confidence: 99%

Embryonic Renal Epithelia: Induction, Nephrogenesis, and Cell Differentiation

Horster

Braun

Huber

1999

Physiological Reviews

184

147

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“…S2a,b). These results indicate that, in addition to endogenous Hoxd3 expression from the caudal myelencephalon to the spinal cord (Tan et al. 1996), the HOXD3 gene exogenously introduced is excessively expressed in the neural tube of the transgenic embryos.…”

Section: Resultsmentioning

confidence: 92%

“…Second, because the amino acid sequence of the HOXD3 protein is 95% identical with that of the mouse Hoxd3 protein (Taniguchi et al. 1992; Tan et al. 1996), it was expected that there would be little difference in function between the two proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, introduction of the human HOXD3 gene would facilitate the detection of HOXD3-positive cells within the neural tube of mouse embryos. Second, because the amino acid sequence of the HOXD3 protein is 95% identical with that of the mouse Hoxd3 protein (Taniguchi et al 1992;Tan et al 1996), it was expected that there would be little difference in function between the two proteins. In fact, as for Hoxd11, Hoxd12 and Hoxd13, the functional equivalence between the mouse and human proteins is verified by the experiment that the mouse vertebral defect caused by deleting Hoxd genes was almost rescued by the introduction of the human DNA including HOXD genes (Spitz et al 2001).…”

Section: Discussionmentioning

confidence: 99%

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Enforced expression of the transcription factor HOXD3 under the control of the Wnt1 regulatory element modulates cell adhesion properties in the developing mouse neural tube

et al. 2011

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HOX genes expressed in a specific spatial and temporal manner play a crucial role in determining the body plan during the early development of vertebrates. In adult tissues, many HOX genes participate in normal hematopoiesis and carcinogenesis. We previously found that overexpression of the homeobox gene HOXD3 alters expression levels of cell adhesion molecules in human cancer cell lines. Here, we have investigated whether HOXD3 expression is related to the cell adhesion processes during mouse development focusing on dorsal midline cells or roofplate cells of the neural tube and neural crest cells. We created transgenic mouse embryos, in which HOXD3 is expressed in the dorsal midline under the control of the Wnt1 regulatory element, and analyzed these embryos at embryonic day 10.5-13.5. In HOXD3-expressing transgenic embryos, although neural crest-derived structures in the trunk region appeared to be normal, striking abnormalities were found in the neural tube. In transgenic embryos expressing the lacZ gene under the control of the Wnt1 regulatory element, expression of lacZ was restricted to roof-plate cells within the neural tube. By contrast, in HOXD3-expressing transgenic embryos, expression of HOXD3 was not only located in the dorsal neural tube, but also had spread inside the ventricular zone in more ventral regions of the neural tube. These findings show that the HOXD3 transgene is expressed more broadly than the Wnt1 gene is normally expressed. Expression of both Wnt1 and Msx1, marker genes in the roof plate, was further extended ventrally in HOXD3-expressing embryos than in normal embryos, suggesting that expression of the HOXD3 transgene expands the roof plate ventrally within the neural tube. In the ventricular zone of HOXD3-expressing embryos at embryonic day 10.5, we observed an increase in the number of mitotic cells and failure of interkinetic nuclear migration of progenitor cells. Furthermore, in HOXD3-expressing embryos at embryonic day 12.5, the ventricular zone, in which progenitor cells became more loosely connected to each other, was composed of a large number of cells that did not express N-cadherin. Our results indicate that expression of HOXD3 is closely associated with modulation of cell-adhesive properties during embryonic development.

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“…Other Hox genes, such as Hoxa-5, Hoxa-6, Hoxd-3, and Hoxd-4, are expressed in the mye!encephalon (Dony and Gruss, 1987;Toth et al, 1987;Gaunt et al, 1989;Tan et al, 1996). Hoxa-4 likewise is detected in the mye!encephalon except that at El5.5 the boundary of expression extends anteriorly next to the limit between medulla and pons (see Fig.…”

Section: Hox Genesmentioning

confidence: 99%

Homeobox Genes in the Developing Mouse Brain

Vollmer

Clerc

1998

Journal of Neurochemistry

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Any list of past and recent findings on vertebrate brain prenatal development would have to include the fundamental roles of homeobox genes, the genes encoding the nuclear regulatory homeodomain proteins. The discovery of homeobox genes and their involvement as master regulatory elements in programing the development of an embryo into a complete adult organism has provided a key to our understanding of ontogenesis. Also, the correlation of mouse developmental mutants and their corresponding human syndromes with mutations in homeobox genes has provided further evidence for the fundamental role of homeobox genes during the vertebrate brain embryonic development. Here, we review the expression patterns and the phenotypes of gene mutations that implicate a large repertoire of mouse homeobox genes in the specification of neuronal functions during brain embryogenesis.

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Sequence and expression of the murine Hoxd-3 homeobox gene.

Cited by 9 publications

References 19 publications

Embryonic Renal Epithelia: Induction, Nephrogenesis, and Cell Differentiation

Embryonic Renal Epithelia: Induction, Nephrogenesis, and Cell Differentiation

Enforced expression of the transcription factor HOXD3 under the control of the Wnt1 regulatory element modulates cell adhesion properties in the developing mouse neural tube

Homeobox Genes in the Developing Mouse Brain

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