Mur (MNS 10) screening with a novel loop‐mediated isothermal amplification assay in Zhongshan, China

Abstract

The LAMP method has identical results with conventional serology method but more suitable for large-scale screening.

The distribution of MNS hybrid glycophorins with Mur antigen expression in Chinese donors including identification of a novel GYP.Bun allele

“…Considering the serious transfusion reaction and haemolytic disease of newborn caused by the anti‐Mi a /Mur, Mur‐positive cells have been added into the antibody screening reagent cells for the routine testing in Taiwan and Hong Kong . In the mainland of China, a series of population studies for Mur antigen distribution show that Mur antigen distributes with a lower frequency (0.5–1.87%) in the Chinese Han from the northern and central regions of China , a higher frequency (5.5–7.55%) in the Chinese Han from the southern regions and a highest frequency (15.4–24.7%) in some minority groups from the south‐western mountain regions of China . The limited reports about the prevalence of anti‐Mur in China also reflect the same trend.…”

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

“…Antisera to detect these antigens are scarce and are not commercially available presenting difficulty to screen for these hybrid glycophorins by serologic methods. With the introduction of molecular biology, DNA‐based methods such as polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) and PCR–sequence‐specific primer, which have been described and applied well previously, direct blood PCR, loop‐mediated isothermal amplification, gold nanoparticle DNA probes, and massive parallel sequencing, have been developed to genotype for specific low‐incidence antigens on hybrid glycophorins. Some of these methods either are laborious and time‐consuming, such as the need for gel electrophoresis, or can only be used for screening but are unable to identify the exact type of hybrid glycophorin.…”

Prevalence of GP. Mur variant phenotype among Malaysian blood donors

“…Bun, and Mur was the most common antigen. [ 12 ] While the anti-MUT plasma was detected in a patient who was admitted to Hospital USM. It was characterized using antibody screening and identification panel cells such as ID-DiaCell I-II-III Asia (Mia+) (Bio-Rad, USA), ID-DiaPanel (IAT and NaCl test) (Bio-Rad, USA), ID-DiaPanel-P (papainized, enzyme test) (Bio-Rad, USA), and Phenocell ™ 0.8% C (Seqirus, Australia) that includes Mur or MUT expressing cells.…”

“…Mur is the main glycophorin variant phenotype expressing Mi a , Mur, and MUT antigens. [ 10 11 12 13 ] It was commonly detected in the populations of China (5%–10%),[ 11 12 ] Taiwan (4%–6%),[ 13 14 ] and Thailand (8%–10%). [ 15 16 ] GP.…”

“…Detection of MNS system glycophorin variant phenotypes is known to be limited to phenotyping reagents, which are not commercially available, and certainly genotyping assays, including polymerase chain reaction (PCR)-based deoxyribonucleic acid (DNA) typing, are applicable as reliable and reproducible alternatives. [ 11 12 13 14 15 21 22 23 24 25 26 27 ] In this study, the target DNA for GP. Mur, GP.…”