Multiwalled‐carbon‐nanotubes‐based matrix solid‐phase dispersion extraction coupled with high‐performance liquid chromatography for the determination of honokiol and magnolol in Magnoliae Cortex

Abstract

In this paper, multiwalled-carbon-nanotube-based matrix solid-phase dispersion coupled to HPLC with diode array detection was used to extract and determine honokiol and magnolol from Magnoliae Cortex. The extraction efficiency of the multiwalled-carbon-nanotube-based matrix solid-phase dispersion was studied and optimized as a function of the amount of dispersing sorbent, volume of elution solvent, and flow rate of elution solvent, with the aid of response surface methodology. An amount of 0.06 g of carboxyl-modified multiwalled carbon nanotubes and 1.5 mL of methanol at a flow rate of 1.1 mL/min were selected. The method obtained good linearity (r(2) > 0.9992) and precision (RSD < 4.7%) for honokiol and magnolol, with limits of detection of 0.045 and 0.087 μg/mL, respectively. The recoveries obtained from analyzing in triplicate spiked samples were determined to be from 90.23 to 101.10% and the RSDs from 3.5 to 4.8%. The proposed method that required less samples and reagents was simpler and faster than Soxhlet and maceration extraction methods. The optimized method was applied for analyzing five real samples collected from different cultivated areas.

Improved oral bioavailability of magnolol by using a binary mixed micelle system

“…The concentration of MO in mixed micelles was measured by HPLC [36], which consisted of a Waters 2695 separation module, a Waters 2489 UV/Vis detector and a reversed-phase C18 column (250 mm  4.6 mm  5 lm) maintained at 30 C; detection was conducted at 290 nm. The mobile phase was mixture of methanol-water (75:25, v/v), and the flow rate was 1.0 ml/min.…”

Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid‐phase dispersion extraction coupled with ultra high‐performance liquid chromatography with photodiode array detection and quadrupole time‐of‐flight mass spectrometry

“…At present, MSPD has attracted increasing interests and has been applied in various industries, such as pesticide residues in fruits and vegetables, preservatives in cosmetics and household products, illegal dyes in food, heavy metal in water, chemicals in human placental tissue, and hazardous chemicals in animal tissue [12][13][14][15][16][17][18][19][20]. More and more applications have been reported in the determination of natural products, especially in herbs [19,[21][22][23][24][25][26][27][28][29]. Compared with conventional extraction methods, MSPD has proven to be a simple, flexible, and versatile strategy with a small aliquot and less time consum-ing [11].…”

Rapid identification and global characterization of multiple constituents from the essential oil of Cortex Dictamni based on GC–MS

“…In recent years, with the rapid development of sensitive, reliable analytical methods and technologies, GC–MS, a powerful hyphenated technique, has proved to be a valuable analytical tool for the rapid on‐line analysis of the known compounds and unknown compounds in TCM . With the advantages of high resolution, high sensitivity as well as high accuracy, GC–MS and serum pharmacochemistry have played a great contribution in TCM analysis, especially in the analysis of essential oil in TCM .…”