Simultaneous determination and qualitative analysis of six types of components in Naoxintong capsule by miniaturized matrix solid‐phase dispersion extraction coupled with ultra high‐performance liquid chromatography with photodiode array detection and quadrupole time‐of‐flight mass spectrometry

Abstract

A simple and effective sample preparation process based on miniaturized matrix solid-phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunic acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol-3-O-rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenanthraquinones (cryptotanshinone), and furans (5-hydroxymethylfurfural) in Naoxintong capsule by ultra high-performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.

Recent advances of green pretreatment techniques for quality control of natural products

“…The role of these adsorbents/dispersants was to break and disperse the analytical sample and purify the interfering medium. Some normal and classical adsorbents have been employed during the MSPD process, such as silica-based materials [7][8][9][10][11][12], C 18 -based materials [13,14], florisil [15][16][17], or their mixed system [11]. These materials have been proved to be efficient and effective to adsorbent targets so that they are always to be applied widely in the extraction of natural products.…”

“…Naoxintong (NXT) capsule has been employed to treat cardiovascular disease as a famous commercial medicinal product. Florisil (100 mg) was used as dispersant to extract multiple active compounds in Naoxintong capsule by MSPD extraction method [15]. Euphorbia fis-cheriana, the root of E. fischeriana Steud.…”

“…Based on the principle of the dissolution in the similar material structure, the polarity of elution solution should be similar to the targets. Besides the small volume of the normal organic reagents were employed during the MSPD process [10,12,15], some novel environmental benign "green solvents" were exploited to elute the analyts after adsorbing. A few scholars applied ILs as elution solvent in MSPD because of high thermal stability, low toxicity, and excellent dissolving capacity in various polarity of solvents [8,9,11,18,24,25].…”

Pharmacokinetics comparison of four major bio-active components in normal and blood stasis rats after administration of Naoxintong Capsule by UPLC-TQ/MS

“…The active chemical ingredients of NXTC mainly include avonoids, avonoid glycosides, phenanthraquinones, phenolic acids, terpenoids, and polysaccharide, which are related to the pharmacological functions of NXTC on inhibition of platelet aggregation, anti-in ammation, reduction of apoptosis and ROS production, activation of lipid metabolism, promotion of angiogenesis and lesion plaque stability, attenuation of vascular calci cation [1,[6][7][8][9]. Many researchers have been trying to explore and identify the major and bioactive constituents of NXTC, and many chemical constituents in NXTC in vitro were quanti ed [10][11][12].…”

A rapid and efficient extraction method based on industrial MCM‐41‐miniaturized matrix solid‐phase dispersion extraction with response surface methodology for simultaneous quantification of six flavonoids in Pollen typhae by ultra‐high‐performance liquid chromatography

“…The solid support material is one of the key factors and the common adsorbents including mussel shell, C18, florisil PR, molecularly imprinted polymers and graphitic fibers . Compared with conventional matrix solid‐phase dispersion extraction, the miniaturized matrix solid‐phase dispersion extraction (MMSPD) has the advantages of easy operation, low consumption of sample and reagent, high‐efficiency, time‐saving, and environment‐friendly . Therefore, it is necessary to use the new adsorbent to enlarge the application of MMSPD for quality evaluation of herbal medicines.…”