Multivariate optimization of capillary electrophoresis methods: A critical review

Orlandini, Serena; Gotti, Roberto; Furlanetto, Sandra

doi:10.1016/j.jpba.2013.04.014

Cited by 89 publications

(44 citation statements)

References 127 publications

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“…The formation of inclusion complexes between the enantiomers and the β-CD is strongly influenced not only by the hydrophobic interaction in the cavity but also by interaction between the hydroxyl groups (or other substituents) on the rim of CDs and substituents of the asymmetric center of the analytes [18]. In our lab, we studied the effect of different β-CD concentrations (5, 10, 15, 20 mM) on the separation performance.…”

Section: Resultsmentioning

confidence: 99%

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

et al. 2014

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A sensitive on-line DPPH-CE-DAD method was developed and validated for both screening and determining the concentration of seven antioxidants of Reduning injection. The pH and concentrations of buffer solution, SDS, β-CD and organic modifier were studied for the detection of DPPH and seven antioxidants. By on-line mixing DPPH and sample solution, a DPPH-CE method for testing the antioxidant activity of the complex matrix was successfully established and used to screen the antioxidant components of Reduning injection. Then, antioxidant components including caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid were quantified by the newly established CE–DAD method. Finally, the total antioxidant activity and the multiple active components were selected as markers to evaluate the quality of Reduning injection. The results demonstrated that the on-line DPPH-CE-DAD method was reagent-saving, rapid and feasible for on-line simultaneous determination of total pharmacological activity and contents of multi-components samples. It was also a powerful method for evaluating the quality control and mechanism of action of TCM injection.

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Section: Resultsmentioning

confidence: 99%

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

et al. 2014

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“…At the beginning of determinations we have selected the type of background electrolyte (BGE) by checking the electrophoretic behavior of the analytes using an acidic BGE -phosphoric acid 25 mM and a basic BGE -25 mM borax. After our prelimary results we selected a 25 mM borax (pH 9.3) as BGE also taking in consideration previous electrophoretic studies [17][18][19][20][21].…”

Section: Resultsmentioning

confidence: 99%

Determination of letrozole, anastrozole and exemestane by capillary zone electrophoresis

Rusu¹,

Hancu²,

Berța³

et al. 2017

Studia UBB Chemia

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ABSTRACT.A new validated capillary zone electrophoresis method was developed for the quantification of three aromatase inhibitors -anastrozole, letrozole and exemestane. After preliminary analysis a sodium tetraborate background electrolyte containing carboxymethyl β-cyclodextrine was selected for the simultaneous determination of the three aromatase inhibitors. 100 mM sodium tetraborate containing 5 mM carboxymethyl β-cyclodextrine as buffer addtive, 25 kV applied voltage, 20 mbar/2 s injection pressure and 25°C temperature were selected as optimum parameters for the determination. Analysis was performed in approximately 10 minutes. Validation parameters, including linearity, precision, detection and quantification limits were determined. Our results prove the applicability of capillary zone electrophoresis for the simultaneous determination of the three aromatase inhibitors from pharmaceutical products. The applicability of the optimized method was also tested for biological samples, proving its reliability for the determination of letrozole without any special treatment of the analyzed spiked urine sample and presenting potential for other biological matrices.

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“…Thus, major microspecies in the range of pH 5 -13 are neutral (100%). It is already known that for conventional CZE method, phosphate and borate buffers can be considered suitable because of their low absorbance at the detection wavelength [32]. Initially, we choose to start with a 25 mM phosphate buffer, an acidic background electrolyte (BGE), but the electrophoretic signal of the substance was difficult to detect (low value of area and height).…”

Section: A Capillary Zone Electrophoresismentioning

confidence: 99%

“…Generally, the BGE concentration change the electrophoretic behavior of the analytes by influencing the selectivity of separation and EOF velocity [32]. The effect of BGE concentration was studied between10 mM to 100 mM.…”

Section: A Capillary Zone Electrophoresismentioning

confidence: 99%

Letrozole Determination by Capillary Zone Electrophoresis and UV Spectrophotometry Methods

Rusu

Sbanca

Todoran

et al. 2017

Acta Medica Marisiensis

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Objective: Letrozole is a highly potent oral nonsteroidal aromatase inhibitor triazole derivative. The aim of this study was to quantify letrozole from bulk, pharmaceutical formulation, and spiked urine samples by developing a simple, rapid and cost effective capillary electrophoresis method. Methods: A capillary zone electrophoresis method was optimized and validated. Additionally, an UV spectrophotometry method was used for comparing results. Results:The capillary zone electrophoresis method using a 90 mM sodium tetraborate background electrolyte proved to be an efficient method for determination of letrozole in a very short time, less than 2 minutes, using 20 kV voltage, 50 mbar/2 seconds pressure and 50°C temperature as optimum parameters. Additionally, the UV spectrophotometry method proved to be simple and efficient to quantify letrozole from bulk material and pharmaceutical formulation with linearity of response between 5 to 20 µg·mL -1 concentrations. For both methods, validation parameters, including linearity, detection and quantification limits were determined. Also we proved that our electrophoretic method has potential in analyzing letrozole from biological samples, obtaining encouraging results on estimation of letrozole from spiked urine samples without any special treatment. Conclusions: To quantify letrozole from bulk material, pharmaceutical preparations, and spiked urine samples the capillary zone electrophoresis method using a tetraborate sodium background electrolyte has proven to be simple and appropriate. Also a simple UV spectrophotometric method has been developed and validated for the same purposes.

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Multivariate optimization of capillary electrophoresis methods: A critical review

Cited by 89 publications

References 127 publications

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

Determination of letrozole, anastrozole and exemestane by capillary zone electrophoresis

Letrozole Determination by Capillary Zone Electrophoresis and UV Spectrophotometry Methods

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