Letrozole Determination by Capillary Zone Electrophoresis and UV Spectrophotometry Methods

Abstract

Objective: Letrozole is a highly potent oral nonsteroidal aromatase inhibitor triazole derivative. The aim of this study was to quantify letrozole from bulk, pharmaceutical formulation, and spiked urine samples by developing a simple, rapid and cost effective capillary electrophoresis method. Methods: A capillary zone electrophoresis method was optimized and validated. Additionally, an UV spectrophotometry method was used for comparing results. Results:The capillary zone electrophoresis method using a 90 mM sodium tetraborate background electrolyte proved to be an efficient method for determination of letrozole in a very short time, less than 2 minutes, using 20 kV voltage, 50 mbar/2 seconds pressure and 50°C temperature as optimum parameters. Additionally, the UV spectrophotometry method proved to be simple and efficient to quantify letrozole from bulk material and pharmaceutical formulation with linearity of response between 5 to 20 µg·mL -1 concentrations. For both methods, validation parameters, including linearity, detection and quantification limits were determined. Also we proved that our electrophoretic method has potential in analyzing letrozole from biological samples, obtaining encouraging results on estimation of letrozole from spiked urine samples without any special treatment. Conclusions: To quantify letrozole from bulk material, pharmaceutical preparations, and spiked urine samples the capillary zone electrophoresis method using a tetraborate sodium background electrolyte has proven to be simple and appropriate. Also a simple UV spectrophotometric method has been developed and validated for the same purposes.

Highly Functionalized Modified Metal Oxides Polymeric Sensors for Potentiometric Determination of Letrozole in Commercial Oral Tablets and Biosamples

“…The action of this medication is very specific and does not inhibit the formation of corticosteroids [25]. LTZ was previously quantified using various analytical techniques, including chromatographic separation such as high-performance thin layer chromatography [26], reversed phase liquid chromatography [27,28], liquid chromatography coupled with tandem mass spectrometry [29,30], and capillary zone electrophoresis [31]. Furthermore, different spectrophotometric methods were reported for the determination of LTZ in various media [32][33][34][35][36].…”

A Review on Different Analytical Methods: Letrozole

“…About 60% of Letrozole gets bound to plasma protein mainly to albumin (55%). [11][12][13][14][15][16][17][18][19][20][21][22][23][24], UFLC [25], UPLC [26] in pharmaceutical formulations and biological samples (Table 3) [27][28][29][30][31], LC-MS/MS (Table 4) [32][33][34][35][36][37][38][39] and Capillary zone electrophoresis [40] for the quantification of Letrozole.…”

A descrição teórica da detecção eletroanalítica do letrozol, assistida pelo compósito VO(OH)-polipirrol, obtido catodicamente

“…Ademais, o seu uso duradouro e excessivo pode provocar osteoporose [11]. Destarte, o desenvolvimento de métodos da sua determinação é, deveras, uma tarefa atual [12][13][14], e o desenvolvimento de métodos eletroquímicos servir-lhe-iam de boa solução.…”