Multivariate Optimization and Validation of a Capillary Electrophoresis Method for the Simultaneous Determination of Dextromethorphan Hydrobromur, Phenylephrine Hydrochloride, Paracetamol and Chlorpheniramine Maleate in a Pharmaceutical Preparation Using Response Surface Methodology

Palabıyık, İsmail Murat; Onur, Feyyaz

doi:10.2116/analsci.26.853

Cited by 21 publications

(13 citation statements)

References 10 publications

(7 reference statements)

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“…EM ¼ 552:2465 À 30:2713x 1 þ 23:8576x 2 þ 158:2195x 3 À 55:2079x 2 1 À 19:2924x 2 2 À 2:5382x 2 3 À 4:9225x 1 x 2 À 4:7425x 2 x 3 À 13:58x 1 x 3 for DOR, EM ¼ 593:8379 þ 15:6577x 1 þ 29:1601x 2 þ 183:614x 3 À 44:5411x 2 1 À 16:6017x 2 2 þ 5:3649x 2 3 À 3:4113x 1 x 2 À 3:1788x 2 x 3 þ 3:7963x 1 x 3 for TIM; By use of these equations containing coefficients, the value of the third factor was calculated to provide the optimum experimental conditions when the other two factors were known [3].…”

Section: Response-surface Methodology For Multivariate Optimizationmentioning

confidence: 99%

“…In addition to chromatographic techniques, capillary electrophoresis has been widely used in recent decades for resolving mixtures and has been used for analysis of multi-component samples with remarkable advantages, for example low sample volume, consumption of small amounts of running buffer, large theoretical plate number, wide working range, and low expense [3].…”

Section: Introductionmentioning

confidence: 99%

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Multivariate Optimization and Validation of a CE method for Simultaneous Analysis of Dorzolamide Hydrochloride and Timolol Maleate in Ophthalmic Solution

2011

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A fast, accurate, precise, and sensitive capillary electrophoretic method has been developed for simultaneous analysis of dorzolamide hydrochloride and timolol maleate in eye drops. Response-surface methodology with a central composite design was used for optimization of buffer pH, applied potential, and temperature. Capillary electrophoresis at 25°C with NaH 2 PO 4 buffer (pH 6.00, 0.025 M), and an applied potential of 20 kV was found to be suitable, and under these optimum conditions, dorzolamide hydrochloride and timolol maleate were separated in approximately 3 min. This method was validated and successfully applied to a pharmaceutical preparation, ophthalmic solution, and the results were compared with those from a spectrophotometric method described in the literature.

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Section: Response-surface Methodology For Multivariate Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multivariate Optimization and Validation of a CE method for Simultaneous Analysis of Dorzolamide Hydrochloride and Timolol Maleate in Ophthalmic Solution

2011

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“…Phenylephrine hydrochloride (PHE), (R)‐2‐methylamino‐1‐3(‐hydroxyphenyl)ethanol hydrochloride (Figure c) is a widely applied effective sympathomimetic agent that has vasoconstrictor effects and is used as a nasal decongestant to relieve severe cold symptoms . Various analytical methods have been reported for the quantitation of PHE in different pharmaceutical forms using spectrophotometric methods, spectrofluorometric methods, HPLC, electrochemical analysis and CE …”

Section: Introductionmentioning

confidence: 99%

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

2020

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Simple and rapid synchronous fluorometric methods were adopted and validated for the simultaneous analysis of a binary mixture of diphenhydramine (DIP) and ibuprofen (IBU) (Mix I) or DIP and phenylephrine (PHE) (Mix II) in their co-formulated pharmaceuticals without prior separation. Analysis of Mix I is based on the measurement of the peak amplitudes (D 1 ) of synchronous fluorescence intensities at 265.1 nm for DIP and 260 nm for IBU. The relationship between the concentration and the amplitude of the first-derivative synchronous fluorescence spectra showed good linearity over the concentration ranges 0.50-10.00 μg ml −1 and 0.50-7.90 μg ml −1 for DIP and IBU, respectively. Analysis of Mix II was based on measurement of the peak amplitude (D 1 ) synchronous fluorescence intensities at 230 nm for DIP and at 253.9 nm for PHE. Moreover, for Mix II, the peak amplitude (D 2 ) synchronous fluorescence intensities were measured at 227.9 nm for DIP and at 264.9 nm for PHE. Calibration plots were rectilinear over the concentration range 0.30-3.50 μg ml −1 and 0.03-0.75 μg ml −1 for DIP and PHE, respectively. The proposed methods were successfully applied to determine the studied compounds in pure form and in pharmaceutical preparations. K E Y W O R D Sibuprofen, diphenhydramine, phenylephrine, synchronous fluorescence, pharmaceutical preparations

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“…It is used mainly as a nasal decongestant either alone or in combination with other agents for the symptomatic relief of cold symptoms . PHE has been previously determined in several pharmaceutical forms using HPLC , CE , spectrophotometry , spectrofluorimetry and electrochemical analysis .…”

Section: Introductionmentioning

confidence: 99%

Derivative emission spectrofluorimetry for the simultaneous determination of guaifenesin and phenylephrine hydrochloride in pharmaceutical tablets

2014

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Rapid, simple and sensitive derivative emission spectrofluorimetric methods have been developed for the simultaneous analysis of binary mixtures of guaifenesin (GUA) and phenylephrine hydrochloride (PHE). The methods are based upon measurement of the native fluorescence intensity of the two drugs at λex = 275 nm in methanolic solutions, followed by differentiation using first (D1) and second (D2) derivative techniques. The derivative fluorescence intensity-concentration plots were rectilinear over a range of 0.1-2 µg/mL for both GUA and PHE. The limits of detection were 0.027 (D1, GUA), 0.025 (D2, GUA), 0.031 (D1, PHE) and 0.033 (D2, PHE) µg/mL and limits of quantitation were 0.089 (D1, GUA), 0.083 (D2, GUA), 0.095 (D1, PHE) and 0.097 (D2, PHE) µg/mL. The proposed derivative emission spectrofluorimetric methods (D1 and D2) were successfully applied for the determination of the two compounds in binary mixtures and tablets with high precision and accuracy. The proposed methods were fully validated as per ICH guidelines.

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Multivariate Optimization and Validation of a Capillary Electrophoresis Method for the Simultaneous Determination of Dextromethorphan Hydrobromur, Phenylephrine Hydrochloride, Paracetamol and Chlorpheniramine Maleate in a Pharmaceutical Preparation Using Response Surface Methodology

Cited by 21 publications

References 10 publications

Multivariate Optimization and Validation of a CE method for Simultaneous Analysis of Dorzolamide Hydrochloride and Timolol Maleate in Ophthalmic Solution

Multivariate Optimization and Validation of a CE method for Simultaneous Analysis of Dorzolamide Hydrochloride and Timolol Maleate in Ophthalmic Solution

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

Derivative emission spectrofluorimetry for the simultaneous determination of guaifenesin and phenylephrine hydrochloride in pharmaceutical tablets

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