Multivariate Assessment of Phenetic Relationships Within the Tribe Luciliini (Diptera: Calliphoridae).

Brown, KR

doi:10.1071/zo9790465

Cited by 8 publications

(5 citation statements)

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“…Their characters described features of the venation rather than the outline shape of the wings as in the present study. Brown (1979) and Brown andShipp (1977, 1978) also used wing vein lengths to examine phenetic relationships within two groups of Australian Diptera and found that the results varied in their consistency with results from previous studies. The previous studies had used standard taxonomic characters including genitalic characters as well as geographic distribution for erecting species groups.…”

Section: Resultsmentioning

confidence: 57%

A Comparison of Fourier Methods for the Description of Wing Shape in Mosquitoes (Diptera: Culicidae)

1984

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Abstract.-Outlines of wings from 127 species of North American mosquitoes were digitized. Comparisons were made among several different methods of reducing the information in the resulting coordinates to a series of descriptors that could be used in multivariate analysis. Methods included Fourier analysis of both radii and tangent angle change functions. In addition, the relatively new method of elliptic Fourier analysis was tried. Cluster and ordination analyses based on the various sets of descriptors summarized well the pattern of similarities and differences in wing shapes, but clusters of similar wings do not agree well with traditional taxonomic groupings. The use of elliptic Fourier descriptors appears to be especially promising for future work.

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Section: Resultsmentioning

confidence: 57%

A Comparison of Fourier Methods for the Description of Wing Shape in Mosquitoes (Diptera: Culicidae)

1984

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“…Since there is no single published phylogeny that encompasses the whole set of species used in this study, smaller subsets of taxonomic groups were chosen as follow: (1) Chrysomya species, compared with the work of , who recovered three clades in their analyses-[clade I] C. semimetallica and C. latifrons; [clade II] C. bezziana, C. pinguis and C. megacephala; and [clade III] C. putoria, C. nigripes, C. varipes, C. flavifrons, C. incisuralis, C. albiceps and C. rufifacies; (2) Lucilia species, compared with different previously published works (Kurahashi 1966;Shepard 1971;Brown 1979;Stevens andWall 1996, 1997;, with most of them supporting separate clades for (L. cuprina, L. sericata), formerly placed in the Phaenicia genus, and (L. eximia, L. bazini, L. ampullacea, L. porphyrina, L. caesar, L. ilustris); (3) Chrysomyinae genera, compared with the work of , which supports the relationships (Chrysomya, (Phormia, Protophormia)) and (Cochliomyia, (Chloroprocta, Hemilucilia)); and (4) Calliphoridae subfamilies, for which the relationship (Chrysomyinae, (Calliphorinae, Luciliinae)) is supported by the molecular analyses of Kutty et al (2010) and also by more traditional classifications based on morphological characters (Shewell 1987, McAlpine 1989-both recognizing the tribe Luciliini inside the subfamily Calliphorinae). Other topologies used in the comparisons were based on Wallman and Donnellan (2001); Wells and Sperling (2001); Stevens and Wall (2001); Otranto and Stevens (2002); Stevens (2003); Chen et al (2004); ; ; Wells and Williams (2007) and Nelson et al (2007).…”

Section: Evaluation Of Its2 Inferred Treesmentioning

confidence: 99%

Evaluation of the internal transcribed spacer 2 (ITS2) as a molecular marker for phylogenetic inference using sequence and secondary structure information in blow flies (Diptera: Calliphoridae)

2011

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The internal transcribed spacer 2 (ITS2) is a small non-coding region located inside the nuclear ribosomal DNA cluster. ITS2 sequence variability is thought to be appropriate to differentiate species and for phylogenetic reconstructions analyses, which can be further improved if structural information is considered. We evaluated the potential of ITS2 as a molecular marker for phylogenetic inference in Calliphoridae (Diptera: Brachycera) using a broad range of inference methods and different substitution models, accounting or not for structural information. Sequence analyses revealed a hierarchically organized pattern of sequence variation and a small level of nucleotide substitution saturation. Intragenomic variation due to small sequence repeats was found mainly in the most variable domain (IV), but it has no significant impact on the phylogenetic signal at the species level. Inferred secondary structures revealed that GC pairs are more frequently found flanking bulges and loops regions in more conserved domains, thus ensuring structure stability. In the phylogenetic analyses, the use of substitution models accounting for structural information significantly improves phylogenetic inference in both neighbour-joining and Bayesian analyses, although the former provides limited resolution for dealing with highly divergent sequences. For Bayesian analyses, a significant improvement in likelihood was observed when considering structure information, although with small changes in topology and overall support, probably reflecting better evolutionary rates estimates. Based on these findings, ITS2 is a suitable molecular marker for phylogenetic analyses in Calliphoridae, at both species and generic level.

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“…Wing morphometrics has been increasingly popular in studies of Diptera, demonstrating the species identi cation value in Syrphidae [24] and forensically important families like Muscidae [25] and Sarcophagidae [26]. It has been proved that wing morphometrics can be used for the identi cation of necrophagous Calliphoridae from Thailand [27] and Europe [28], and distinguish different genus and species of Calliphoridae, Cochliomyia [29], Lucilia [23,30] and Chrysomya [29,31]. In addition, geometric morphometric analysis can be used to distinguish variability between geographical populations [29,32], seasonal morphs [33] or sexes [26,34] among the same species, and recently it is used to identify Piophilidae (Diptera) for forensic purpose [35].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Molecular Identification and Wing Morphometrics of Forensically Important Blow Flies in China

li¹,

Chen²,

Shang³

et al. 2020

Preprint

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Background: Correct species identification is the most crucial step in applying entomological evidence to estimate the postmortem interval (PMI) since death of decomposed corpses. Wing morphometrics have been proposed in species classification as an alternative method of traditional morphology and molecular approaches. However, so far, this method has not been applied to the identification of Chinese Calliphoridae and few studies compare the two identification methods.Methods: We used landmark-based geometric morphometrics of wings to identify nine medically and forensically important blow fly species of China. 270 specimens representing nine species and eight genera were sampled, 18 landmarks on the right wing were measured and analyzed using canonical variates analysis and discriminant function analysis. Then, a cross-validation test was used to evaluate reliability of the method. Moreover, in order to further assess the validity of this method, molecular identification is used for comparative analysis. Eighty sequences of cytochrome c oxidase subunit I (COI) of Calliphoridae isolated from different countries were downloaded from Genbank, including the data previously submitted by our team. Results: Different species and genera can be well separated through morphometric analysis with an overall classification accuracy of 80~100%, but discrimination between sexes was less effective. The results indicated that the discriminative efficiency of the two methods is almost identical.Conclusions: Wing morphometrics can be used as a complementary method of molecular identification for the geographical location and gender identification of certain species as a simple and cheap method.

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Multivariate Assessment of Phenetic Relationships Within the Tribe Luciliini (Diptera: Calliphoridae).

Cited by 8 publications

References 0 publications

A Comparison of Fourier Methods for the Description of Wing Shape in Mosquitoes (Diptera: Culicidae)

A Comparison of Fourier Methods for the Description of Wing Shape in Mosquitoes (Diptera: Culicidae)

Evaluation of the internal transcribed spacer 2 (ITS2) as a molecular marker for phylogenetic inference using sequence and secondary structure information in blow flies (Diptera: Calliphoridae)

Comparative Analysis of Molecular Identification and Wing Morphometrics of Forensically Important Blow Flies in China

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